Genetic Analysis of Variation for Carotenoids and Tocopherols in Maize
type: CreativeWork
description: Carotenoids and tocopherols are two classes of compounds present in maize (Zea mays L.) which may add value to the grain. Both the carotenoids and tocopherols are fat soluble antioxidant vitamins. The vitamin and antioxidant properties of the carotenoids and tocopherols provide health and economic benefits which potentially could be captured by both producers and consumers. beta-carotene and alpha-tocopherol have the highest biological activity for the carotenoids and the tocopherols, respectively. However, neither of these compounds is in high concentration in maize kernels. The objective of this research was to gain basic genetic information about the chromosomal regions with quantitative trait loci (QTL) controlling carotenoid and tocopherol accumulation in maize kernels using molecular markers. Once the QTL are identified and validated, they could be used in a marker assisted selection program to facilitate increasing levels of these antioxidant vitamins in maize grain. Three sets of materials were assayed in this research (W64a x A632) F 2:4 mapping population, testcross progeny with AE335, and (IHO90 x B73) B73 BC1S1 mapping population. Molecular markers were evaluated on the F2:4 and BC1S1 populations, and linkage maps were created. Each population was analyzed for four carotenoids, beta-carotene, beta-cryptoxanthin, lutein, and zeaxanthin and three tocopherols, alpha-tocopherol, delta-tocopherol, and gamma-tocopherol. Composite Interval Mapping was used to identify QTL. Total carotenoids and total tocopherols were calculated to identify QTL with affects on the total concentration of carotenoids or tocopherols. Ratio (alpha/gamma) tocopherol was analyzed to identify QTL influencing the conversion of gamma-tocopherol to alpha-tocopherol. For the tocopherols, two chromosomal regions with QTL on chromosomes 1 and 5 were significant for the three sets of materials. For the carotenoids three regions with QTL on chromosomes 6, 7, and 8 were significant across popul…
abstract: Carotenoids and tocopherols are two classes of compounds present in maize (Zea mays L.) which may add value to the grain. Both the carotenoids and tocopherols are fat soluble antioxidant vitamins. The vitamin and antioxidant properties of the carotenoids and tocopherols provide health and economic benefits which potentially could be captured by both producers and consumers. beta-carotene and alpha-tocopherol have the highest biological activity for the carotenoids and the tocopherols, respectively. However, neither of these compounds is in high concentration in maize kernels. The objective of this research was to gain basic genetic information about the chromosomal regions with quantitative trait loci (QTL) controlling carotenoid and tocopherol accumulation in maize kernels using molecular markers. Once the QTL are identified and validated, they could be used in a marker assisted selection program to facilitate increasing levels of these antioxidant vitamins in maize grain. Three sets of materials were assayed in this research (W64a x A632) F 2:4 mapping population, testcross progeny with AE335, and (IHO90 x B73) B73 BC1S1 mapping population. Molecular markers were evaluated on the F2:4 and BC1S1 populations, and linkage maps were created. Each population was analyzed for four carotenoids, beta-carotene, beta-cryptoxanthin, lutein, and zeaxanthin and three tocopherols, alpha-tocopherol, delta-tocopherol, and gamma-tocopherol. Composite Interval Mapping was used to identify QTL. Total carotenoids and total tocopherols were calculated to identify QTL with affects on the total concentration of carotenoids or tocopherols. Ratio (alpha/gamma) tocopherol was analyzed to identify QTL influencing the conversion of gamma-tocopherol to alpha-tocopherol. For the tocopherols, two chromosomal regions with QTL on chromosomes 1 and 5 were significant for the three sets of materials. For the carotenoids three regions with QTL on chromosomes 6, 7, and 8 were significant across popul…
author: Wong, Jeffrey Cleighton
language: eng
keywords: Biology, Genetics
date published: 2015-09-25T22:33:25Z
date modified: 2026-07-22T22:26:24Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/85003