{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84953"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84953","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Regulation of Estrogen Receptor Alpha Expression in Breast Cancer Cells by Sulforaphane","abstract":"Sulforaphane (SUL, 1-isothiocyanato-4-(methylsulfinyl) butane) is an isothiocyanate (ITC) derived from glucoraphanin and is abundant in broccoli and broccoli sprouts and it has a variety of potential chemopreventive actions. SUL is capable of inhibiting cancer at both early (initiation) and late (promotion) stages of tumorigenesis. In this study we analyzed the effects of SUL on the cell proliferation of several human breast cancer cell lines and evaluated the effect of SUL on estrogen receptor alpha (ERalpha) protein and mRNA expression in MCF-7 cells. Our results show that SUL inhibits cell growth and proliferation in several breast cancer cell lines, and that SUL decreases ERalpha protein expression via suppression of mRNA at the transcriptional level as well as increasing proteasome-mediated receptor degradation. These data provide important molecular insight into a possible mechanism by which SUL inhibits cell growth and proliferation in hormone receptor containing breast cancer cells such as MCF-7.","abstract_html":"Sulforaphane (SUL, 1-isothiocyanato-4-(methylsulfinyl) butane) is an isothiocyanate (ITC) derived from glucoraphanin and is abundant in broccoli and broccoli sprouts and it has a variety of potential chemopreventive actions. SUL is capable of inhibiting cancer at both early (initiation) and late (promotion) stages of tumorigenesis. In this study we analyzed the effects of SUL on the cell proliferation of several human breast cancer cell lines and evaluated the effect of SUL on estrogen receptor alpha (ERalpha) protein and mRNA expression in MCF-7 cells. Our results show that SUL inhibits cell growth and proliferation in several breast cancer cell lines, and that SUL decreases ERalpha protein expression via suppression of mRNA at the transcriptional level as well as increasing proteasome-mediated receptor degradation. These data provide important molecular insight into a possible mechanism by which SUL inhibits cell growth and proliferation in hormone receptor containing breast cancer cells such as MCF-7.","abstract_has_math":false,"creators":["Cortes Ramirez, Marcela"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Nutritional Sciences","degree_department":null,"school":null,"contributors":["Singletary, Keith W."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:31:07Z","date_published":"2015-09-25T22:31:07Z","updated_at":"2026-07-22T22:26:24Z","subjects":["Health Sciences, Nutrition"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3290210"],"render_values":[{"text":"(MiAaPQ)AAI3290210","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84953","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Singletary, Keith W."]},{"key":"dc:creator","label":"Author","values":["Cortes Ramirez, Marcela"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:31:07Z","10000-01-01","2007"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Nutritional Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Health Sciences, Nutrition"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/84953","(MiAaPQ)AAI3290210"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Sulforaphane (SUL, 1-isothiocyanato-4-(methylsulfinyl) butane) is an isothiocyanate (ITC) derived from glucoraphanin and is abundant in broccoli and broccoli sprouts and it has a variety of potential chemopreventive actions. SUL is capable of inhibiting cancer at both early (initiation) and late (promotion) stages of tumorigenesis. In this study we analyzed the effects of SUL on the cell proliferation of several human breast cancer cell lines and evaluated the effect of SUL on estrogen receptor alpha (ERalpha) protein and mRNA expression in MCF-7 cells. Our results show that SUL inhibits cell growth and proliferation in several breast cancer cell lines, and that SUL decreases ERalpha protein expression via suppression of mRNA at the transcriptional level as well as increasing proteasome-mediated receptor degradation. These data provide important molecular insight into a possible mechanism by which SUL inhibits cell growth and proliferation in hormone receptor containing breast cancer cells such as MCF-7.","Made available in DSpace on 2015-09-25T22:31:07Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3290210.pdf: 3336210 bytes, checksum: eb55aad3ebf1eb21014ba5901c1d7701 (MD5) Previous issue date: 2007","Embargo set by: Seth Robbins for item 86234 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","130 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2007."]},{"key":"dc:title","label":"Title","values":["Regulation of Estrogen Receptor Alpha Expression in Breast Cancer Cells by Sulforaphane"]}]}],"canonical_facts":{"dc:contributor":["Singletary, Keith W."],"dc:creator":["Cortes Ramirez, Marcela"],"dc:date":["2015-09-25T22:31:07Z","10000-01-01","2007"],"dc:description":["Sulforaphane (SUL, 1-isothiocyanato-4-(methylsulfinyl) butane) is an isothiocyanate (ITC) derived from glucoraphanin and is abundant in broccoli and broccoli sprouts and it has a variety of potential chemopreventive actions. SUL is capable of inhibiting cancer at both early (initiation) and late (promotion) stages of tumorigenesis. In this study we analyzed the effects of SUL on the cell proliferation of several human breast cancer cell lines and evaluated the effect of SUL on estrogen receptor alpha (ERalpha) protein and mRNA expression in MCF-7 cells. Our results show that SUL inhibits cell growth and proliferation in several breast cancer cell lines, and that SUL decreases ERalpha protein expression via suppression of mRNA at the transcriptional level as well as increasing proteasome-mediated receptor degradation. These data provide important molecular insight into a possible mechanism by which SUL inhibits cell growth and proliferation in hormone receptor containing breast cancer cells such as MCF-7.","Made available in DSpace on 2015-09-25T22:31:07Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3290210.pdf: 3336210 bytes, checksum: eb55aad3ebf1eb21014ba5901c1d7701 (MD5) Previous issue date: 2007","Embargo set by: Seth Robbins for item 86234 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","130 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2007."],"dc:identifier":["http://hdl.handle.net/2142/84953","(MiAaPQ)AAI3290210"],"dc:language":["eng"],"dc:subject":["Health Sciences, Nutrition"],"dc:title":["Regulation of Estrogen Receptor Alpha Expression in Breast Cancer Cells by Sulforaphane"],"dc:type":["text"],"thesis:degree_discipline":["Nutritional Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:24Z"}