{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84886"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84886","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characterization of a T Cell Receptor: Peptide/MHC Binding Interaction","abstract":"To begin to examine the 2C TCR residues which provide a significant thermodynamic contribution toward the binding energy of a variety of ligands, including monoclonal antibodies (mAb), superantigens, and peptide/MHC ligands, we have expressed a family of 2C scTCRs which contain alanine point mutations in the complementarity determining regions (CDR) and fourth hypervariable region of the $\\beta$ chain. Using these mutant scTCRs, we have identified V$\\beta$ residues which are energetically important in the interaction of the 2C TCR with the anti-V$\\beta$8 mAb F23.2, the anti-clonotypic mAb 1B2, and the peptide/MHC ligand QL9/L$\\rm\\sp{d}.$ Residues from all three CDR$\\beta$ loops were implicated in the binding of the 2C TCR to QL9/L$\\rm\\sp{d}.$ Furthermore, every residue identified as part of the QL9/L$\\rm\\sp{d}$ epitope was also identified as part of the 1B2 epitope, suggesting that these two TCR ligands may share certain features in their tertiary structure even though their primary and secondary sequences are quite different. The construction of this panel of V$\\beta$ mutant scTCRs has also facilitated possible future identification of 2C TCR $\\beta$ chain residues which contact other peptide/MHC, mAb, and superantigen ligands.","abstract_html":"To begin to examine the 2C TCR residues which provide a significant thermodynamic contribution toward the binding energy of a variety of ligands, including monoclonal antibodies (mAb), superantigens, and peptide/MHC ligands, we have expressed a family of 2C scTCRs which contain alanine point mutations in the complementarity determining regions (CDR) and fourth hypervariable region of the <span class=\"etd-inline-math\">&beta;</span> chain. Using these mutant scTCRs, we have identified V<span class=\"etd-inline-math\">&beta;</span> residues which are energetically important in the interaction of the 2C TCR with the anti-V<span class=\"etd-inline-math\">&beta;</span>8 mAb F23.2, the anti-clonotypic mAb 1B2, and the peptide/MHC ligand QL9/L$\\rm\\sp{d}.$ Residues from all three CDR<span class=\"etd-inline-math\">&beta;</span> loops were implicated in the binding of the 2C TCR to QL9/L$\\rm\\sp{d}.$ Furthermore, every residue identified as part of the QL9/L$\\rm\\sp{d}$ epitope was also identified as part of the 1B2 epitope, suggesting that these two TCR ligands may share certain features in their tertiary structure even though their primary and secondary sequences are quite different. The construction of this panel of V<span class=\"etd-inline-math\">&beta;</span> mutant scTCRs has also facilitated possible future identification of 2C TCR <span class=\"etd-inline-math\">&beta;</span> chain residues which contact other peptide/MHC, mAb, and superantigen ligands.","abstract_has_math":true,"creators":["Schlueter, Carol Joy"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Kranz, David M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:28:21Z","date_published":"2015-09-25T22:28:21Z","updated_at":"2026-07-22T22:26:24Z","subjects":["Health Sciences, Immunology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI9737243"],"render_values":[{"text":"(MiAaPQ)AAI9737243","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84886","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kranz, David M."]},{"key":"dc:creator","label":"Author","values":["Schlueter, Carol Joy"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:28:21Z","10000-01-01","1997"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Health Sciences, Immunology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/84886","(MiAaPQ)AAI9737243"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["To begin to examine the 2C TCR residues which provide a significant thermodynamic contribution toward the binding energy of a variety of ligands, including monoclonal antibodies (mAb), superantigens, and peptide/MHC ligands, we have expressed a family of 2C scTCRs which contain alanine point mutations in the complementarity determining regions (CDR) and fourth hypervariable region of the $\\beta$ chain. Using these mutant scTCRs, we have identified V$\\beta$ residues which are energetically important in the interaction of the 2C TCR with the anti-V$\\beta$8 mAb F23.2, the anti-clonotypic mAb 1B2, and the peptide/MHC ligand QL9/L$\\rm\\sp{d}.$ Residues from all three CDR$\\beta$ loops were implicated in the binding of the 2C TCR to QL9/L$\\rm\\sp{d}.$ Furthermore, every residue identified as part of the QL9/L$\\rm\\sp{d}$ epitope was also identified as part of the 1B2 epitope, suggesting that these two TCR ligands may share certain features in their tertiary structure even though their primary and secondary sequences are quite different. The construction of this panel of V$\\beta$ mutant scTCRs has also facilitated possible future identification of 2C TCR $\\beta$ chain residues which contact other peptide/MHC, mAb, and superantigen ligands.","Made available in DSpace on 2015-09-25T22:28:21Z (GMT). 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Using these mutant scTCRs, we have identified V$\\beta$ residues which are energetically important in the interaction of the 2C TCR with the anti-V$\\beta$8 mAb F23.2, the anti-clonotypic mAb 1B2, and the peptide/MHC ligand QL9/L$\\rm\\sp{d}.$ Residues from all three CDR$\\beta$ loops were implicated in the binding of the 2C TCR to QL9/L$\\rm\\sp{d}.$ Furthermore, every residue identified as part of the QL9/L$\\rm\\sp{d}$ epitope was also identified as part of the 1B2 epitope, suggesting that these two TCR ligands may share certain features in their tertiary structure even though their primary and secondary sequences are quite different. The construction of this panel of V$\\beta$ mutant scTCRs has also facilitated possible future identification of 2C TCR $\\beta$ chain residues which contact other peptide/MHC, mAb, and superantigen ligands.","Made available in DSpace on 2015-09-25T22:28:21Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9737243.pdf: 10919322 bytes, checksum: 6525b2a2fe1712d6ccd67f74937324d5 (MD5) Previous issue date: 1997","Embargo set by: Seth Robbins for item 86167 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","268 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997."],"dc:identifier":["http://hdl.handle.net/2142/84886","(MiAaPQ)AAI9737243"],"dc:language":["eng"],"dc:subject":["Health Sciences, Immunology"],"dc:title":["Characterization of a T Cell Receptor: Peptide/MHC Binding Interaction"],"dc:type":["text"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:24Z"}