{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84875"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84875","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"T Cell Receptor Alpha Chain Residues Involved in Antibody Binding and Recognition of peptide/MHC Ligand","abstract":"Mutant 2C scTCRs were further examined in ligand binding experiments. The 2C TCR recognizes a peptide (QL9) presented by the MHC product L$\\sp{\\rm d}$. Four alanine mutations which affected mAb binding also disrupted binding to the QL9/L$\\sp{\\rm d}$ complex by greater than ten-fold relative to the wild-type scTCR. A fifth $\\alpha$ chain mutation, located within a loop that is analogous to the fourth hypervariable region (HV4) of the $\\beta$ chain, disrupted binding to QL9/L$\\sp{\\rm d}$ approximately five-fold. Based on these results and a homology model of the 2C $\\alpha$ chain variable region, these five residues are likely to be involved in either stabilization of CDR loops or in direct contact with the peptide/MHC ligand.","abstract_html":"Mutant 2C scTCRs were further examined in ligand binding experiments. The 2C TCR recognizes a peptide (QL9) presented by the MHC product L$\\sp{\\rm d}$. Four alanine mutations which affected mAb binding also disrupted binding to the QL9/L$\\sp{\\rm d}$ complex by greater than ten-fold relative to the wild-type scTCR. A fifth <span class=\"etd-inline-math\">&alpha;</span> chain mutation, located within a loop that is analogous to the fourth hypervariable region (HV4) of the <span class=\"etd-inline-math\">&beta;</span> chain, disrupted binding to QL9/L$\\sp{\\rm d}$ approximately five-fold. Based on these results and a homology model of the 2C <span class=\"etd-inline-math\">&alpha;</span> chain variable region, these five residues are likely to be involved in either stabilization of CDR loops or in direct contact with the peptide/MHC ligand.","abstract_has_math":true,"creators":["Brodnicki, Thomas Charles"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Kranz, David M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:28:17Z","date_published":"2015-09-25T22:28:17Z","updated_at":"2026-07-22T22:26:24Z","subjects":["Health Sciences, Immunology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI9717257"],"render_values":[{"text":"(MiAaPQ)AAI9717257","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84875","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kranz, David M."]},{"key":"dc:creator","label":"Author","values":["Brodnicki, Thomas Charles"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:28:17Z","10000-01-01","1997"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Health Sciences, Immunology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/84875","(MiAaPQ)AAI9717257"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Mutant 2C scTCRs were further examined in ligand binding experiments. The 2C TCR recognizes a peptide (QL9) presented by the MHC product L$\\sp{\\rm d}$. Four alanine mutations which affected mAb binding also disrupted binding to the QL9/L$\\sp{\\rm d}$ complex by greater than ten-fold relative to the wild-type scTCR. A fifth $\\alpha$ chain mutation, located within a loop that is analogous to the fourth hypervariable region (HV4) of the $\\beta$ chain, disrupted binding to QL9/L$\\sp{\\rm d}$ approximately five-fold. Based on these results and a homology model of the 2C $\\alpha$ chain variable region, these five residues are likely to be involved in either stabilization of CDR loops or in direct contact with the peptide/MHC ligand.","Made available in DSpace on 2015-09-25T22:28:17Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9717257.pdf: 8485594 bytes, checksum: 0144c71bd1505211d340bcfd5f8d4cdb (MD5) Previous issue date: 1997","Embargo set by: Seth Robbins for item 86156 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","195 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997."]},{"key":"dc:title","label":"Title","values":["T Cell Receptor Alpha Chain Residues Involved in Antibody Binding and Recognition of peptide/MHC Ligand"]}]}],"canonical_facts":{"dc:contributor":["Kranz, David M."],"dc:creator":["Brodnicki, Thomas Charles"],"dc:date":["2015-09-25T22:28:17Z","10000-01-01","1997"],"dc:description":["Mutant 2C scTCRs were further examined in ligand binding experiments. The 2C TCR recognizes a peptide (QL9) presented by the MHC product L$\\sp{\\rm d}$. Four alanine mutations which affected mAb binding also disrupted binding to the QL9/L$\\sp{\\rm d}$ complex by greater than ten-fold relative to the wild-type scTCR. A fifth $\\alpha$ chain mutation, located within a loop that is analogous to the fourth hypervariable region (HV4) of the $\\beta$ chain, disrupted binding to QL9/L$\\sp{\\rm d}$ approximately five-fold. Based on these results and a homology model of the 2C $\\alpha$ chain variable region, these five residues are likely to be involved in either stabilization of CDR loops or in direct contact with the peptide/MHC ligand.","Made available in DSpace on 2015-09-25T22:28:17Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9717257.pdf: 8485594 bytes, checksum: 0144c71bd1505211d340bcfd5f8d4cdb (MD5) Previous issue date: 1997","Embargo set by: Seth Robbins for item 86156 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","195 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1997."],"dc:identifier":["http://hdl.handle.net/2142/84875","(MiAaPQ)AAI9717257"],"dc:language":["eng"],"dc:subject":["Health Sciences, Immunology"],"dc:title":["T Cell Receptor Alpha Chain Residues Involved in Antibody Binding and Recognition of peptide/MHC Ligand"],"dc:type":["text"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:24Z"}