{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84859"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84859","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characterization of the Chemoreceptors in the Bacillus Subtilis Chemotaxis System","abstract":"Physical characterization of the chemoreceptors was probed by identification of a zinc ion binding area, using isothermal titration calorimetry. In addition, in vivo chemotactic assays showed that altering the charges of the methylation sites on the chemoreceptors can have a significant effect on the activity of the receptor. Furthermore, individual methylation sites were shown to be responsible for both changes in the affinity of the chemoreceptor for attractant and receptor activation. These findings suggest a novel, more complicated role for methylation than previously suggested, and lead to a clearer picture of the coordination of the three adaptational systems in the B. subtilis chemotactic sensory transduction.","abstract_html":"Physical characterization of the chemoreceptors was probed by identification of a zinc ion binding area, using isothermal titration calorimetry. In addition, in vivo chemotactic assays showed that altering the charges of the methylation sites on the chemoreceptors can have a significant effect on the activity of the receptor. Furthermore, individual methylation sites were shown to be responsible for both changes in the affinity of the chemoreceptor for attractant and receptor activation. These findings suggest a novel, more complicated role for methylation than previously suggested, and lead to a clearer picture of the coordination of the three adaptational systems in the B. subtilis chemotactic sensory transduction.","abstract_has_math":false,"creators":["Glekas, George D."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["Ordal, George W."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:28:13Z","date_published":"2015-09-25T22:28:13Z","updated_at":"2026-07-22T22:26:24Z","subjects":["Chemistry, Biochemistry"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3347388"],"render_values":[{"text":"(MiAaPQ)AAI3347388","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84859","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Ordal, George W."]},{"key":"dc:creator","label":"Author","values":["Glekas, George D."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:28:13Z","10000-01-01","2008"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/84859","(MiAaPQ)AAI3347388"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Physical characterization of the chemoreceptors was probed by identification of a zinc ion binding area, using isothermal titration calorimetry. In addition, in vivo chemotactic assays showed that altering the charges of the methylation sites on the chemoreceptors can have a significant effect on the activity of the receptor. Furthermore, individual methylation sites were shown to be responsible for both changes in the affinity of the chemoreceptor for attractant and receptor activation. These findings suggest a novel, more complicated role for methylation than previously suggested, and lead to a clearer picture of the coordination of the three adaptational systems in the B. subtilis chemotactic sensory transduction.","Made available in DSpace on 2015-09-25T22:28:13Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3347388.pdf: 2431295 bytes, checksum: 33ba03cc226af57ebd17d76edcdca23b (MD5) Previous issue date: 2008","Embargo set by: Seth Robbins for item 86140 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","146 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008."]},{"key":"dc:title","label":"Title","values":["Characterization of the Chemoreceptors in the Bacillus Subtilis Chemotaxis System"]}]}],"canonical_facts":{"dc:contributor":["Ordal, George W."],"dc:creator":["Glekas, George D."],"dc:date":["2015-09-25T22:28:13Z","10000-01-01","2008"],"dc:description":["Physical characterization of the chemoreceptors was probed by identification of a zinc ion binding area, using isothermal titration calorimetry. In addition, in vivo chemotactic assays showed that altering the charges of the methylation sites on the chemoreceptors can have a significant effect on the activity of the receptor. Furthermore, individual methylation sites were shown to be responsible for both changes in the affinity of the chemoreceptor for attractant and receptor activation. These findings suggest a novel, more complicated role for methylation than previously suggested, and lead to a clearer picture of the coordination of the three adaptational systems in the B. subtilis chemotactic sensory transduction.","Made available in DSpace on 2015-09-25T22:28:13Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3347388.pdf: 2431295 bytes, checksum: 33ba03cc226af57ebd17d76edcdca23b (MD5) Previous issue date: 2008","Embargo set by: Seth Robbins for item 86140 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","146 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008."],"dc:identifier":["http://hdl.handle.net/2142/84859","(MiAaPQ)AAI3347388"],"dc:language":["eng"],"dc:subject":["Chemistry, Biochemistry"],"dc:title":["Characterization of the Chemoreceptors in the Bacillus Subtilis Chemotaxis System"],"dc:type":["text"],"thesis:degree_discipline":["Biochemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:24Z"}