{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84814"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84814","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characterization of Membrane Organization and Dynamics in Giant Unilamellar Vesicles Assembled From Rat Kidney Membrane Extracts and Fragments","abstract":"Fluctuation correlation spectroscopy (FCS) measurements performed on the intact BBM reveal that the protein-lipid dynamics in renal BBM is complex with a large range of diffusing species. In particular, the dynamics of NaPi-IIa was related to the perturbations in the BBM as a result of dietary potassium deficiency. By calculating the diffusion coefficient of NaPi-IIa and using photon counting histogram (PCH) analysis to measure protein aggregation, we determined that the lateral diffusion of NaPi-IIa is slowed down and its aggregation is increased in potassium deficiency, both of which are associated with the decreased Na/Pi cotransport activity. This model was proposed as a means for posttranslational regulation of Na/Pi cotransport activity that is mediated by the increased sphingomyelin and glycosphingolipid content in potassium-deficient BBM, although the mechanisms by which these lipids may modulate NaPi-IIa activity have yet to be determined.","abstract_html":"Fluctuation correlation spectroscopy (FCS) measurements performed on the intact BBM reveal that the protein-lipid dynamics in renal BBM is complex with a large range of diffusing species. In particular, the dynamics of NaPi-IIa was related to the perturbations in the BBM as a result of dietary potassium deficiency. By calculating the diffusion coefficient of NaPi-IIa and using photon counting histogram (PCH) analysis to measure protein aggregation, we determined that the lateral diffusion of NaPi-IIa is slowed down and its aggregation is increased in potassium deficiency, both of which are associated with the decreased Na/Pi cotransport activity. This model was proposed as a means for posttranslational regulation of Na/Pi cotransport activity that is mediated by the increased sphingomyelin and glycosphingolipid content in potassium-deficient BBM, although the mechanisms by which these lipids may modulate NaPi-IIa activity have yet to be determined.","abstract_has_math":false,"creators":["Cheng, Melanie Anne B."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biochemistry","degree_department":null,"school":null,"contributors":["William W. 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Mantulin"],"dc:creator":["Cheng, Melanie Anne B."],"dc:date":["2015-09-25T22:28:02Z","10000-01-01","2005"],"dc:description":["Fluctuation correlation spectroscopy (FCS) measurements performed on the intact BBM reveal that the protein-lipid dynamics in renal BBM is complex with a large range of diffusing species. In particular, the dynamics of NaPi-IIa was related to the perturbations in the BBM as a result of dietary potassium deficiency. By calculating the diffusion coefficient of NaPi-IIa and using photon counting histogram (PCH) analysis to measure protein aggregation, we determined that the lateral diffusion of NaPi-IIa is slowed down and its aggregation is increased in potassium deficiency, both of which are associated with the decreased Na/Pi cotransport activity. This model was proposed as a means for posttranslational regulation of Na/Pi cotransport activity that is mediated by the increased sphingomyelin and glycosphingolipid content in potassium-deficient BBM, although the mechanisms by which these lipids may modulate NaPi-IIa activity have yet to be determined.","Made available in DSpace on 2015-09-25T22:28:02Z (GMT). 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