Expression Profiling of Transgenic Arabidopsis Over- and Underexpressing SR45, a Plant-Specific Serine /Arginine -Rich Factor
type: CreativeWork
description: The effects of SR45 depletion on other transcripts have been studied using RT-PCR analyses and more recently using oligoarray approaches. In plants completely silenced for the transgenic mGFP-SR45 transcript and nearly completely silenced for the endogenous SR45 transcript, several other splicing factor transcripts are downregulated or alternatively spliced. Global oligoarray profiling of an initial set of plants overexpressing or underexpressing SR45 transcripts has indicated that SR45 depletion downregulates a number of important transport factors (nucleoporin 155, exportin 1) as well as several stress-related transcripts that may represent secondary downstream targets. As a result of these secondary effects, it is likely that SR45-silenced plants are more susceptible to stress.
abstract: The effects of SR45 depletion on other transcripts have been studied using RT-PCR analyses and more recently using oligoarray approaches. In plants completely silenced for the transgenic mGFP-SR45 transcript and nearly completely silenced for the endogenous SR45 transcript, several other splicing factor transcripts are downregulated or alternatively spliced. Global oligoarray profiling of an initial set of plants overexpressing or underexpressing SR45 transcripts has indicated that SR45 depletion downregulates a number of important transport factors (nucleoporin 155, exportin 1) as well as several stress-related transcripts that may represent secondary downstream targets. As a result of these secondary effects, it is likely that SR45-silenced plants are more susceptible to stress.
author: Balasubramanian, Dheepa
language: eng
keywords: Chemistry, Biochemistry
date published: 2015-09-25T22:28:01Z
date modified: 2026-07-22T22:26:24Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/84810