Expression Profiling of Transgenic Arabidopsis Over- and Underexpressing SR45, a Plant-Specific Serine /Arginine -Rich Factor
The effects of SR45 depletion on other transcripts have been studied using RT-PCR analyses and more recently using oligoarray approaches. In plants completely silenced for the transgenic mGFP-SR45 transcript and nearly completely silenced for the endogenous SR45 transcript, several other splicing factor transcripts are downregulated or alternatively spliced. Global oligoarray profiling of an initial set of plants overexpressing or underexpressing SR45 transcripts has indicated that SR45 depletion downregulates a number of important transport factors (nucleoporin 155, exportin 1) as well as several stress-related transcripts that may represent secondary downstream targets. As a result of these secondary effects, it is likely that SR45-silenced plants are more susceptible to stress.
University of Illinois - Urbana-Champaign
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University of Illinois at Urbana-Champaign