{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84454"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84454","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Mass Spectrometric Characterization of Peptides in Neurons","abstract":"The MALDI-based approach is inherently different from conventional methods, in that essentially all peptides present at significant concentrations are detected, not only those which are pre-selected or radioactively tagged. Furthermore, single cells, nerves, and vesicles are screened without the need to isolate each peptide from its complex cellular environment. Sampling, data acquisition, and strategies for the generation of molecular ion images are presented which reveal the utility of MALDI MS for direct and rapid neuropeptide characterization.","abstract_html":"The MALDI-based approach is inherently different from conventional methods, in that essentially all peptides present at significant concentrations are detected, not only those which are pre-selected or radioactively tagged. Furthermore, single cells, nerves, and vesicles are screened without the need to isolate each peptide from its complex cellular environment. Sampling, data acquisition, and strategies for the generation of molecular ion images are presented which reveal the utility of MALDI MS for direct and rapid neuropeptide characterization.","abstract_has_math":false,"creators":["Garden, Rebecca Wells"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Sweedler, Jonathan V."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:14:34Z","date_published":"2015-09-25T22:14:34Z","updated_at":"2026-07-22T22:26:23Z","subjects":["Chemistry, Analytical"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI9953020"],"render_values":[{"text":"(MiAaPQ)AAI9953020","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84454","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Sweedler, Jonathan V."]},{"key":"dc:creator","label":"Author","values":["Garden, Rebecca Wells"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:14:34Z","10000-01-01","1999"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Analytical"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/84454","(MiAaPQ)AAI9953020"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The MALDI-based approach is inherently different from conventional methods, in that essentially all peptides present at significant concentrations are detected, not only those which are pre-selected or radioactively tagged. Furthermore, single cells, nerves, and vesicles are screened without the need to isolate each peptide from its complex cellular environment. Sampling, data acquisition, and strategies for the generation of molecular ion images are presented which reveal the utility of MALDI MS for direct and rapid neuropeptide characterization.","Made available in DSpace on 2015-09-25T22:14:34Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9953020.pdf: 11223069 bytes, checksum: eeca73d265144602e84380a356134d6d (MD5) Previous issue date: 1999","Embargo set by: Seth Robbins for item 85735 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","231 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999."]},{"key":"dc:title","label":"Title","values":["Mass Spectrometric Characterization of Peptides in Neurons"]}]}],"canonical_facts":{"dc:contributor":["Sweedler, Jonathan V."],"dc:creator":["Garden, Rebecca Wells"],"dc:date":["2015-09-25T22:14:34Z","10000-01-01","1999"],"dc:description":["The MALDI-based approach is inherently different from conventional methods, in that essentially all peptides present at significant concentrations are detected, not only those which are pre-selected or radioactively tagged. Furthermore, single cells, nerves, and vesicles are screened without the need to isolate each peptide from its complex cellular environment. Sampling, data acquisition, and strategies for the generation of molecular ion images are presented which reveal the utility of MALDI MS for direct and rapid neuropeptide characterization.","Made available in DSpace on 2015-09-25T22:14:34Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9953020.pdf: 11223069 bytes, checksum: eeca73d265144602e84380a356134d6d (MD5) Previous issue date: 1999","Embargo set by: Seth Robbins for item 85735 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","231 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999."],"dc:identifier":["http://hdl.handle.net/2142/84454","(MiAaPQ)AAI9953020"],"dc:language":["eng"],"dc:subject":["Chemistry, Analytical"],"dc:title":["Mass Spectrometric Characterization of Peptides in Neurons"],"dc:type":["text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:23Z"}