Development of Assays for Neuronal Peptide Content and Release
type: CreativeWork
description: Neuropeptide release is also studied with MALDI TOF MS. In this method, a small chamber (10 $\mu$l) is used to hold an isolated bag cell cluster in a small volume of physiological saline. The chamber consists of a standard pipette tip with the narrow end melted around two sections of fused silica capillaries. Physiological saline flows into and from the chamber via capillaries and gravimetric pressure flow. Following electrical stimulation of a bag cell cluster, 50-100 nl samples are collected from the outlet end of the sampling capillary for analysis. MALDI MS is performed on the sample for peptide detection. Even in high salt concentrations peptides are detected by MALDI MS.
abstract: Neuropeptide release is also studied with MALDI TOF MS. In this method, a small chamber (10 $\mu$l) is used to hold an isolated bag cell cluster in a small volume of physiological saline. The chamber consists of a standard pipette tip with the narrow end melted around two sections of fused silica capillaries. Physiological saline flows into and from the chamber via capillaries and gravimetric pressure flow. Following electrical stimulation of a bag cell cluster, 50-100 nl samples are collected from the outlet end of the sampling capillary for analysis. MALDI MS is performed on the sample for peptide detection. Even in high salt concentrations peptides are detected by MALDI MS.
author: Shippy, Scott Allen
language: eng
keywords: Biology, Neuroscience
date published: 2015-09-25T22:14:16Z
date modified: 2026-07-22T22:26:23Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/84391