{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84282"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84282","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Investigations of Serotonin Metabolism in Mammalian and Other Deuterostome Model Systems","abstract":"Here the metabolism of 5-HT has been investigated in higher animals, the deuterostomes, which included the invertebrates Xenoturbella bocki, a marine worm, and Strongylocentrotus purppuratus, the sea urchin as well as mammals such as Rattus norvegicus. Studies were performed using a highly specialized capillary electrophoresis (CE) system designed to detect 5-HT and related compounds at attomole levels. The invertebrate deuterstomes investigated were found to metabolize 5-HT in a manner similar to both more primitive invertebrates and to vertebrates; results include the discovery of metabolites such as gammaglutamyl 5-HT, 5-HT sulfate (5-HT-SO4) and 5-hydroxyindole acetic acid (5-HIAA) in these organisms. A novel 5-HT metabolite in the rat (Rattus norvegicus ), 5-hydroxyindole thiazolidine carboxylic acid (5-HITCA), was characterized via mass spectrometry (MS) and further investigated using CE. Levels of 5-HT and some of its key metabolites were also investigated in this mammal under conditions of induced serotonin syndrome, a physiological condition caused by an excessive increase in 5-HT levels often associated with a combination of antidepressant therapies. This research contributes to our understanding of the metabolic pathways of 5-HT in both healthy and disease states. The findings aid in understanding the 5-HT link to various disorders.","abstract_html":"Here the metabolism of 5-HT has been investigated in higher animals, the deuterostomes, which included the invertebrates Xenoturbella bocki, a marine worm, and Strongylocentrotus purppuratus, the sea urchin as well as mammals such as Rattus norvegicus. Studies were performed using a highly specialized capillary electrophoresis (CE) system designed to detect 5-HT and related compounds at attomole levels. The invertebrate deuterstomes investigated were found to metabolize 5-HT in a manner similar to both more primitive invertebrates and to vertebrates; results include the discovery of metabolites such as gammaglutamyl 5-HT, 5-HT sulfate (5-HT-SO4) and 5-hydroxyindole acetic acid (5-HIAA) in these organisms. A novel 5-HT metabolite in the rat (Rattus norvegicus ), 5-hydroxyindole thiazolidine carboxylic acid (5-HITCA), was characterized via mass spectrometry (MS) and further investigated using CE. Levels of 5-HT and some of its key metabolites were also investigated in this mammal under conditions of induced serotonin syndrome, a physiological condition caused by an excessive increase in 5-HT levels often associated with a combination of antidepressant therapies. This research contributes to our understanding of the metabolic pathways of 5-HT in both healthy and disease states. The findings aid in understanding the 5-HT link to various disorders.","abstract_has_math":false,"creators":["Squires, Leah N."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Jonathan Sweedler"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:13:47Z","date_published":"2015-09-25T22:13:47Z","updated_at":"2026-07-22T22:26:22Z","subjects":["Chemistry, Analytical"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3290389"],"render_values":[{"text":"(MiAaPQ)AAI3290389","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84282","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Jonathan Sweedler"]},{"key":"dc:creator","label":"Author","values":["Squires, Leah N."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:13:47Z","10000-01-01","2007"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Analytical"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/84282","(MiAaPQ)AAI3290389"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Here the metabolism of 5-HT has been investigated in higher animals, the deuterostomes, which included the invertebrates Xenoturbella bocki, a marine worm, and Strongylocentrotus purppuratus, the sea urchin as well as mammals such as Rattus norvegicus. Studies were performed using a highly specialized capillary electrophoresis (CE) system designed to detect 5-HT and related compounds at attomole levels. The invertebrate deuterstomes investigated were found to metabolize 5-HT in a manner similar to both more primitive invertebrates and to vertebrates; results include the discovery of metabolites such as gammaglutamyl 5-HT, 5-HT sulfate (5-HT-SO4) and 5-hydroxyindole acetic acid (5-HIAA) in these organisms. A novel 5-HT metabolite in the rat (Rattus norvegicus ), 5-hydroxyindole thiazolidine carboxylic acid (5-HITCA), was characterized via mass spectrometry (MS) and further investigated using CE. Levels of 5-HT and some of its key metabolites were also investigated in this mammal under conditions of induced serotonin syndrome, a physiological condition caused by an excessive increase in 5-HT levels often associated with a combination of antidepressant therapies. 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Studies were performed using a highly specialized capillary electrophoresis (CE) system designed to detect 5-HT and related compounds at attomole levels. The invertebrate deuterstomes investigated were found to metabolize 5-HT in a manner similar to both more primitive invertebrates and to vertebrates; results include the discovery of metabolites such as gammaglutamyl 5-HT, 5-HT sulfate (5-HT-SO4) and 5-hydroxyindole acetic acid (5-HIAA) in these organisms. A novel 5-HT metabolite in the rat (Rattus norvegicus ), 5-hydroxyindole thiazolidine carboxylic acid (5-HITCA), was characterized via mass spectrometry (MS) and further investigated using CE. Levels of 5-HT and some of its key metabolites were also investigated in this mammal under conditions of induced serotonin syndrome, a physiological condition caused by an excessive increase in 5-HT levels often associated with a combination of antidepressant therapies. 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