Immobilization of Catalytic DNA for Advanced Materials and Sensors
The DNAzyme was also immobilized on patterned glass surfaces via covalent attachment. Substrate-functionalized 100 nm polystyrene particles were then hybridized in the absence and presence of Pb2+. The result was the Pb2+-mediated tethering of particles onto patterned grids, where Pb2+ induced the cleavage of the DNAzyme complex and prevented assembly of the particles. The assembly of these advanced materials was monitored in real-time using laser diffraction and was characterized via scanning electron microscopy. Addition of 10 muM Pb2+ showed >99% inhibition of assembly.
University of Illinois - Urbana-Champaign
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University of Illinois at Urbana-Champaign