{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84251"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84251","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Analysis of D-Aspartate as a Signaling Molecule in the Aplysia Californica Central Nervous System Using Capillary Electrophoresis and Radioisotopic Labeling","abstract":"A second cell-cell signaling molecule investigated is D-aspartate (D-Asp). D-Asp is present in the nervous systems of both vertebrates and invertebrates, and its biosynthesis has been observed in mammalian cells. We suspect that D-Asp acts as a classical neurotransmitter in the central nervous system. The mollusk Aplysia californica has shown high levels of D-Asp in the neural ganglia; and this, along with its relatively simple invertebrate system, makes Aplysia a viable model system for studying the function and neurochemistry of D-Asp. Using Aplysia californica , we have investigated the localization of D-Asp as well as its synthesis from L-Asp. We developed a technique utilizing off-line CE coupled with radionuclide detection, which enables high sensitivity characterization of L- to D-Asp conversion. Furthermore, these capillary electrophoretic techniques, along with liquid scintillation counting and MALDI-MS analysis, have allowed us to address other criteria required to determine whether D-Asp acts as a classical neurotransmitter, including release from cells, sodium dependence of uptake, response to D-Asp by tissues, and the D-Asp stimulated release of cardiomodulatory peptides from the R3-14 cells. These techniques developed provide robust methods for the analysis of neurotransmitters and elucidation of their chiral moieties in complex mass-limited biological samples.","abstract_html":"A second cell-cell signaling molecule investigated is D-aspartate (D-Asp). D-Asp is present in the nervous systems of both vertebrates and invertebrates, and its biosynthesis has been observed in mammalian cells. We suspect that D-Asp acts as a classical neurotransmitter in the central nervous system. The mollusk Aplysia californica has shown high levels of D-Asp in the neural ganglia; and this, along with its relatively simple invertebrate system, makes Aplysia a viable model system for studying the function and neurochemistry of D-Asp. Using Aplysia californica , we have investigated the localization of D-Asp as well as its synthesis from L-Asp. We developed a technique utilizing off-line CE coupled with radionuclide detection, which enables high sensitivity characterization of L- to D-Asp conversion. Furthermore, these capillary electrophoretic techniques, along with liquid scintillation counting and MALDI-MS analysis, have allowed us to address other criteria required to determine whether D-Asp acts as a classical neurotransmitter, including release from cells, sodium dependence of uptake, response to D-Asp by tissues, and the D-Asp stimulated release of cardiomodulatory peptides from the R3-14 cells. These techniques developed provide robust methods for the analysis of neurotransmitters and elucidation of their chiral moieties in complex mass-limited biological samples.","abstract_has_math":false,"creators":["Scanlan, Cory Randolph"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Sweedler, Jonathan V."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:13:39Z","date_published":"2015-09-25T22:13:39Z","updated_at":"2026-07-22T22:26:22Z","subjects":["Chemistry, Analytical"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3250319"],"render_values":[{"text":"(MiAaPQ)AAI3250319","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84251","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Sweedler, Jonathan V."]},{"key":"dc:creator","label":"Author","values":["Scanlan, Cory Randolph"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:13:39Z","10000-01-01","2006"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Analytical"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/84251","(MiAaPQ)AAI3250319"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["A second cell-cell signaling molecule investigated is D-aspartate (D-Asp). D-Asp is present in the nervous systems of both vertebrates and invertebrates, and its biosynthesis has been observed in mammalian cells. We suspect that D-Asp acts as a classical neurotransmitter in the central nervous system. The mollusk Aplysia californica has shown high levels of D-Asp in the neural ganglia; and this, along with its relatively simple invertebrate system, makes Aplysia a viable model system for studying the function and neurochemistry of D-Asp. Using Aplysia californica , we have investigated the localization of D-Asp as well as its synthesis from L-Asp. We developed a technique utilizing off-line CE coupled with radionuclide detection, which enables high sensitivity characterization of L- to D-Asp conversion. Furthermore, these capillary electrophoretic techniques, along with liquid scintillation counting and MALDI-MS analysis, have allowed us to address other criteria required to determine whether D-Asp acts as a classical neurotransmitter, including release from cells, sodium dependence of uptake, response to D-Asp by tissues, and the D-Asp stimulated release of cardiomodulatory peptides from the R3-14 cells. These techniques developed provide robust methods for the analysis of neurotransmitters and elucidation of their chiral moieties in complex mass-limited biological samples.","Made available in DSpace on 2015-09-25T22:13:39Z (GMT). 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D-Asp is present in the nervous systems of both vertebrates and invertebrates, and its biosynthesis has been observed in mammalian cells. We suspect that D-Asp acts as a classical neurotransmitter in the central nervous system. The mollusk Aplysia californica has shown high levels of D-Asp in the neural ganglia; and this, along with its relatively simple invertebrate system, makes Aplysia a viable model system for studying the function and neurochemistry of D-Asp. Using Aplysia californica , we have investigated the localization of D-Asp as well as its synthesis from L-Asp. We developed a technique utilizing off-line CE coupled with radionuclide detection, which enables high sensitivity characterization of L- to D-Asp conversion. Furthermore, these capillary electrophoretic techniques, along with liquid scintillation counting and MALDI-MS analysis, have allowed us to address other criteria required to determine whether D-Asp acts as a classical neurotransmitter, including release from cells, sodium dependence of uptake, response to D-Asp by tissues, and the D-Asp stimulated release of cardiomodulatory peptides from the R3-14 cells. These techniques developed provide robust methods for the analysis of neurotransmitters and elucidation of their chiral moieties in complex mass-limited biological samples.","Made available in DSpace on 2015-09-25T22:13:39Z (GMT). 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