{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84225"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84225","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Structure and Function of Colicin E5 Ribonuclease Domain","abstract":"The binding of ImmE5 to ColE5 resulted in a 50% increase of fluorescence emissions. The isothermal titration calorimetry studies showed a -10 kcal/mol enthalpy change upon the formation of ColE5•ImmE5 complex. The mutational studies of ImmE5 indicated that ImmE5 K4 and D95 contributes a larger inhibitory effect toward ColE5. The structure comparison of ColE5 in homodimer and complex showed that ImmE5 not only blocked but also closed the cleft of the active site of ColE5.","abstract_html":"The binding of ImmE5 to ColE5 resulted in a 50% increase of fluorescence emissions. The isothermal titration calorimetry studies showed a -10 kcal/mol enthalpy change upon the formation of ColE5•ImmE5 complex. The mutational studies of ImmE5 indicated that ImmE5 K4 and D95 contributes a larger inhibitory effect toward ColE5. The structure comparison of ColE5 in homodimer and complex showed that ImmE5 not only blocked but also closed the cleft of the active site of ColE5.","abstract_has_math":false,"creators":["Lin, Yi-Lun"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Raven Huang"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:13:33Z","date_published":"2015-09-25T22:13:33Z","updated_at":"2026-07-22T22:26:22Z","subjects":["Biology, Molecular"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3223658"],"render_values":[{"text":"(MiAaPQ)AAI3223658","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84225","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Raven Huang"]},{"key":"dc:creator","label":"Author","values":["Lin, Yi-Lun"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:13:33Z","10000-01-01","2006"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/84225","(MiAaPQ)AAI3223658"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The binding of ImmE5 to ColE5 resulted in a 50% increase of fluorescence emissions. 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