{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84122"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84122","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"From Peptide Precursors to Lanthionine -Containing Peptide Antibiotics Using the First Isolated and Active Lacticin 481 Synthetase: Mechanistic Studies and Peptide Engineering","abstract":"The putative dehydratase SpaB involved in the biosynthesis of subtilin was heterologously overexpressed in E. coli and purified by metal-chelated affinity chromatography. However, in vitro reconstitution using the purified SpaB, SpaC, and the substrate SpaS was unsuccessful. As a result, the biosynthesis of lacticin 481 which employs one single modification enzyme presumably responsible for both dehydration and cyclization was investigated. Both the modification enzyme LctM and the substrate LctA were cloned and purified to homogeneity. The in vitro reconstitution of LctM, the lacticin 481 synthetase, was successful. The assay product was characterized in detail through tandem mass spectrometry, suggesting the formation of identical structural motifs found in lacticin 481. Therefore, LctM is indeed a bifunctional protein, performing both dehydration and cyclization reactions. This study presents the very first in vitro biosynthetic system of any lantibiotic. In addition, upon treatment with the protease Lys-C, the modified peptide in the absence of the leader sequence and the first Lys residue showed biological activity. Mechanistic studies with the active LctM disclosed that the activity of LctM absolutely requires both Mg2+ and ATP. The products of ATP were determined to be ADP and phosphate. The enzyme has low substrate specificity as a series of LctA mutants proved substrates for LctM. Furthermore, peptide engineering was performed, and a large number of lacticin 481 analogs were generated. From these studies, additional structural and mechanistic insights have been elaborated.","abstract_html":"The putative dehydratase SpaB involved in the biosynthesis of subtilin was heterologously overexpressed in E. coli and purified by metal-chelated affinity chromatography. However, in vitro reconstitution using the purified SpaB, SpaC, and the substrate SpaS was unsuccessful. As a result, the biosynthesis of lacticin 481 which employs one single modification enzyme presumably responsible for both dehydration and cyclization was investigated. Both the modification enzyme LctM and the substrate LctA were cloned and purified to homogeneity. The in vitro reconstitution of LctM, the lacticin 481 synthetase, was successful. The assay product was characterized in detail through tandem mass spectrometry, suggesting the formation of identical structural motifs found in lacticin 481. Therefore, LctM is indeed a bifunctional protein, performing both dehydration and cyclization reactions. This study presents the very first in vitro biosynthetic system of any lantibiotic. In addition, upon treatment with the protease Lys-C, the modified peptide in the absence of the leader sequence and the first Lys residue showed biological activity. Mechanistic studies with the active LctM disclosed that the activity of LctM absolutely requires both Mg2+ and ATP. The products of ATP were determined to be ADP and phosphate. The enzyme has low substrate specificity as a series of LctA mutants proved substrates for LctM. Furthermore, peptide engineering was performed, and a large number of lacticin 481 analogs were generated. From these studies, additional structural and mechanistic insights have been elaborated.","abstract_has_math":false,"creators":["Xie, Lili"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["van der Donk, Wilfred A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:13:02Z","date_published":"2015-09-25T22:13:02Z","updated_at":"2026-07-22T22:26:22Z","subjects":["Biology, Molecular"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3101996"],"render_values":[{"text":"(MiAaPQ)AAI3101996","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84122","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["van der Donk, Wilfred A."]},{"key":"dc:creator","label":"Author","values":["Xie, Lili"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:13:02Z","10000-01-01","2003"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Molecular"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/84122","(MiAaPQ)AAI3101996"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The putative dehydratase SpaB involved in the biosynthesis of subtilin was heterologously overexpressed in E. coli and purified by metal-chelated affinity chromatography. However, in vitro reconstitution using the purified SpaB, SpaC, and the substrate SpaS was unsuccessful. As a result, the biosynthesis of lacticin 481 which employs one single modification enzyme presumably responsible for both dehydration and cyclization was investigated. Both the modification enzyme LctM and the substrate LctA were cloned and purified to homogeneity. The in vitro reconstitution of LctM, the lacticin 481 synthetase, was successful. The assay product was characterized in detail through tandem mass spectrometry, suggesting the formation of identical structural motifs found in lacticin 481. Therefore, LctM is indeed a bifunctional protein, performing both dehydration and cyclization reactions. This study presents the very first in vitro biosynthetic system of any lantibiotic. In addition, upon treatment with the protease Lys-C, the modified peptide in the absence of the leader sequence and the first Lys residue showed biological activity. Mechanistic studies with the active LctM disclosed that the activity of LctM absolutely requires both Mg2+ and ATP. The products of ATP were determined to be ADP and phosphate. The enzyme has low substrate specificity as a series of LctA mutants proved substrates for LctM. Furthermore, peptide engineering was performed, and a large number of lacticin 481 analogs were generated. From these studies, additional structural and mechanistic insights have been elaborated.","Made available in DSpace on 2015-09-25T22:13:02Z (GMT). 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However, in vitro reconstitution using the purified SpaB, SpaC, and the substrate SpaS was unsuccessful. As a result, the biosynthesis of lacticin 481 which employs one single modification enzyme presumably responsible for both dehydration and cyclization was investigated. Both the modification enzyme LctM and the substrate LctA were cloned and purified to homogeneity. The in vitro reconstitution of LctM, the lacticin 481 synthetase, was successful. The assay product was characterized in detail through tandem mass spectrometry, suggesting the formation of identical structural motifs found in lacticin 481. Therefore, LctM is indeed a bifunctional protein, performing both dehydration and cyclization reactions. This study presents the very first in vitro biosynthetic system of any lantibiotic. In addition, upon treatment with the protease Lys-C, the modified peptide in the absence of the leader sequence and the first Lys residue showed biological activity. Mechanistic studies with the active LctM disclosed that the activity of LctM absolutely requires both Mg2+ and ATP. The products of ATP were determined to be ADP and phosphate. The enzyme has low substrate specificity as a series of LctA mutants proved substrates for LctM. Furthermore, peptide engineering was performed, and a large number of lacticin 481 analogs were generated. From these studies, additional structural and mechanistic insights have been elaborated.","Made available in DSpace on 2015-09-25T22:13:02Z (GMT). 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