{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/83530"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/83530","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Nuclear Transfer With Porcine Embryonic Stem Cells","abstract":"Results from embryo culture experiments indicated: (1) bovine serum albumin (BSA) supplementation improved developmental rates of four- and eight-cell Meishan embryos to the blastocyst stage, (2) fetal bovine serum supplementation improved hatching rates of later stage Meishan embryos, (3) fatty acid-free BSA did not improve developmental rates of early stage embryos, (4) more Meishan than Yorkshire one-cell embryos developed to the eight-cell and compact morula stages following 96 hours of culture, (5) more Yorkshire than Meishan two-cell embryos developed to the eight-cell stage following 96 hours of culture and (6) modified Whitten's medium +1.5% BSA was the most efficient medium for culture of one-cell embryos to the blastocyst stage. Results from activation and culture of in vivo matured oocytes indicated: (1) ethanol had no effect on activation rates, (2) cold shock, sham enucleation, electroactivation and electroactivation + culture with cycloheximide improved activation rates, and (3) electroactivation + culture with cycloheximide was the most efficient treatment for production of pronuclear stage cytoplasm. Results from NT experiments indicated that neither enucleated, unactivated oocytes nor enucleated, activated oocytes promoted development of NT embryos constructed from porcine ES cells beyond the four-cell stage. However, enucleated zygotes supported development of NT embryos beyond the four-cell stage to the compact morula stage. These results provide preliminary evidence for in vitro development of NT embryos produced from porcine ES cells.","abstract_html":"Results from embryo culture experiments indicated: (1) bovine serum albumin (BSA) supplementation improved developmental rates of four- and eight-cell Meishan embryos to the blastocyst stage, (2) fetal bovine serum supplementation improved hatching rates of later stage Meishan embryos, (3) fatty acid-free BSA did not improve developmental rates of early stage embryos, (4) more Meishan than Yorkshire one-cell embryos developed to the eight-cell and compact morula stages following 96 hours of culture, (5) more Yorkshire than Meishan two-cell embryos developed to the eight-cell stage following 96 hours of culture and (6) modified Whitten's medium +1.5% BSA was the most efficient medium for culture of one-cell embryos to the blastocyst stage. Results from activation and culture of in vivo matured oocytes indicated: (1) ethanol had no effect on activation rates, (2) cold shock, sham enucleation, electroactivation and electroactivation + culture with cycloheximide improved activation rates, and (3) electroactivation + culture with cycloheximide was the most efficient treatment for production of pronuclear stage cytoplasm. Results from NT experiments indicated that neither enucleated, unactivated oocytes nor enucleated, activated oocytes promoted development of NT embryos constructed from porcine ES cells beyond the four-cell stage. However, enucleated zygotes supported development of NT embryos beyond the four-cell stage to the compact morula stage. These results provide preliminary evidence for in vitro development of NT embryos produced from porcine ES cells.","abstract_has_math":false,"creators":["White, Brett Rodger"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Animal Sciences","degree_department":null,"school":null,"contributors":["Wheeler, Matthew B."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T21:07:58Z","date_published":"2015-09-25T21:07:58Z","updated_at":"2026-07-22T22:26:21Z","subjects":["Biology, Animal Physiology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3017009"],"render_values":[{"text":"(MiAaPQ)AAI3017009","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/83530","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Wheeler, Matthew B."]},{"key":"dc:creator","label":"Author","values":["White, Brett Rodger"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T21:07:58Z","10000-01-01","1997"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Animal Physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/83530","(MiAaPQ)AAI3017009"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Results from embryo culture experiments indicated: (1) bovine serum albumin (BSA) supplementation improved developmental rates of four- and eight-cell Meishan embryos to the blastocyst stage, (2) fetal bovine serum supplementation improved hatching rates of later stage Meishan embryos, (3) fatty acid-free BSA did not improve developmental rates of early stage embryos, (4) more Meishan than Yorkshire one-cell embryos developed to the eight-cell and compact morula stages following 96 hours of culture, (5) more Yorkshire than Meishan two-cell embryos developed to the eight-cell stage following 96 hours of culture and (6) modified Whitten's medium +1.5% BSA was the most efficient medium for culture of one-cell embryos to the blastocyst stage. Results from activation and culture of in vivo matured oocytes indicated: (1) ethanol had no effect on activation rates, (2) cold shock, sham enucleation, electroactivation and electroactivation + culture with cycloheximide improved activation rates, and (3) electroactivation + culture with cycloheximide was the most efficient treatment for production of pronuclear stage cytoplasm. Results from NT experiments indicated that neither enucleated, unactivated oocytes nor enucleated, activated oocytes promoted development of NT embryos constructed from porcine ES cells beyond the four-cell stage. However, enucleated zygotes supported development of NT embryos beyond the four-cell stage to the compact morula stage. These results provide preliminary evidence for in vitro development of NT embryos produced from porcine ES cells.","Made available in DSpace on 2015-09-25T21:07:58Z (GMT). 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Results from activation and culture of in vivo matured oocytes indicated: (1) ethanol had no effect on activation rates, (2) cold shock, sham enucleation, electroactivation and electroactivation + culture with cycloheximide improved activation rates, and (3) electroactivation + culture with cycloheximide was the most efficient treatment for production of pronuclear stage cytoplasm. Results from NT experiments indicated that neither enucleated, unactivated oocytes nor enucleated, activated oocytes promoted development of NT embryos constructed from porcine ES cells beyond the four-cell stage. However, enucleated zygotes supported development of NT embryos beyond the four-cell stage to the compact morula stage. These results provide preliminary evidence for in vitro development of NT embryos produced from porcine ES cells.","Made available in DSpace on 2015-09-25T21:07:58Z (GMT). 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