{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/83101"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/83101","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characterization and Expression of Plant Defense Genes in Peach and Tomato","abstract":"The influence of three different agar concentrations, 0.8, 1.0, and 1.2%, on tomato transformation efficiency was examined. Cotyledons of Lycopersicon esculentum cv. 'Sweet Chelsea' were cocultivated with Agrobacterium tumefaciens strain GV3101 harboring derivatives if the binary vector pCAMBIA 2301. The binary vectors pGR-1, pChi-1, pRIP-1, and pAFP-1 carried a maize beta-glucanase, maize chitinase, iris ribosome-inactivating protein (RIP), or an antimicrobial protein gene, respectively, each driven by the CaMV 35S promoter. Transformation was initially verified by the presence of an nptII gene and GUS enzyme activity in the uidA reporter gene, both engineered into the gene construct. Further confirmation of the transgenics was accomplished by Southern blot hybridization. Lines containing the transgenes were exposed to the tomato fungal pathogen Alternaria solani. The plants were assessed for pathogen susceptibility. Selected beta-glucanase and Mj-AMP lines were more resistant to early blight than non-transformed lines.","abstract_html":"The influence of three different agar concentrations, 0.8, 1.0, and 1.2%, on tomato transformation efficiency was examined. Cotyledons of Lycopersicon esculentum cv. 'Sweet Chelsea' were cocultivated with Agrobacterium tumefaciens strain GV3101 harboring derivatives if the binary vector pCAMBIA 2301. The binary vectors pGR-1, pChi-1, pRIP-1, and pAFP-1 carried a maize beta-glucanase, maize chitinase, iris ribosome-inactivating protein (RIP), or an antimicrobial protein gene, respectively, each driven by the CaMV 35S promoter. Transformation was initially verified by the presence of an nptII gene and GUS enzyme activity in the uidA reporter gene, both engineered into the gene construct. Further confirmation of the transgenics was accomplished by Southern blot hybridization. Lines containing the transgenes were exposed to the tomato fungal pathogen Alternaria solani. The plants were assessed for pathogen susceptibility. Selected beta-glucanase and Mj-AMP lines were more resistant to early blight than non-transformed lines.","abstract_has_math":false,"creators":["Schaefer, Scott Charles"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Natural Resrouces and Environmental Sciences","degree_department":null,"school":null,"contributors":["Korban, Schuyler S."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T21:00:29Z","date_published":"2015-09-25T21:00:29Z","updated_at":"2026-07-22T22:26:20Z","subjects":["Agriculture, Plant Pathology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3160949"],"render_values":[{"text":"(MiAaPQ)AAI3160949","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/83101","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Korban, Schuyler S."]},{"key":"dc:creator","label":"Author","values":["Schaefer, Scott Charles"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T21:00:29Z","10000-01-01","2004"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Natural Resrouces and Environmental Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Plant Pathology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/83101","(MiAaPQ)AAI3160949"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The influence of three different agar concentrations, 0.8, 1.0, and 1.2%, on tomato transformation efficiency was examined. Cotyledons of Lycopersicon esculentum cv. 'Sweet Chelsea' were cocultivated with Agrobacterium tumefaciens strain GV3101 harboring derivatives if the binary vector pCAMBIA 2301. The binary vectors pGR-1, pChi-1, pRIP-1, and pAFP-1 carried a maize beta-glucanase, maize chitinase, iris ribosome-inactivating protein (RIP), or an antimicrobial protein gene, respectively, each driven by the CaMV 35S promoter. Transformation was initially verified by the presence of an nptII gene and GUS enzyme activity in the uidA reporter gene, both engineered into the gene construct. Further confirmation of the transgenics was accomplished by Southern blot hybridization. Lines containing the transgenes were exposed to the tomato fungal pathogen Alternaria solani. The plants were assessed for pathogen susceptibility. Selected beta-glucanase and Mj-AMP lines were more resistant to early blight than non-transformed lines.","Made available in DSpace on 2015-09-25T21:00:29Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3160949.pdf: 6722512 bytes, checksum: dfbceb6848ea11b4a9f6aa08a71ababd (MD5) Previous issue date: 2004","Embargo set by: Seth Robbins for item 84382 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","115 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2004."]},{"key":"dc:title","label":"Title","values":["Characterization and Expression of Plant Defense Genes in Peach and Tomato"]}]}],"canonical_facts":{"dc:contributor":["Korban, Schuyler S."],"dc:creator":["Schaefer, Scott Charles"],"dc:date":["2015-09-25T21:00:29Z","10000-01-01","2004"],"dc:description":["The influence of three different agar concentrations, 0.8, 1.0, and 1.2%, on tomato transformation efficiency was examined. Cotyledons of Lycopersicon esculentum cv. 'Sweet Chelsea' were cocultivated with Agrobacterium tumefaciens strain GV3101 harboring derivatives if the binary vector pCAMBIA 2301. The binary vectors pGR-1, pChi-1, pRIP-1, and pAFP-1 carried a maize beta-glucanase, maize chitinase, iris ribosome-inactivating protein (RIP), or an antimicrobial protein gene, respectively, each driven by the CaMV 35S promoter. Transformation was initially verified by the presence of an nptII gene and GUS enzyme activity in the uidA reporter gene, both engineered into the gene construct. Further confirmation of the transgenics was accomplished by Southern blot hybridization. Lines containing the transgenes were exposed to the tomato fungal pathogen Alternaria solani. The plants were assessed for pathogen susceptibility. Selected beta-glucanase and Mj-AMP lines were more resistant to early blight than non-transformed lines.","Made available in DSpace on 2015-09-25T21:00:29Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3160949.pdf: 6722512 bytes, checksum: dfbceb6848ea11b4a9f6aa08a71ababd (MD5) Previous issue date: 2004","Embargo set by: Seth Robbins for item 84382 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","115 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2004."],"dc:identifier":["http://hdl.handle.net/2142/83101","(MiAaPQ)AAI3160949"],"dc:language":["eng"],"dc:subject":["Agriculture, Plant Pathology"],"dc:title":["Characterization and Expression of Plant Defense Genes in Peach and Tomato"],"dc:type":["text"],"thesis:degree_discipline":["Natural Resrouces and Environmental Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:20Z"}