{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/83077"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/83077","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Developing a Genetic Transformation Protocol for American Grapes (Vitis X Lambruscana) and Transforming Vitis to Produce Parthenocarpic Fruits","abstract":"The main goal of this research was to transform Vitis. x labruscana L. H. Bailey ('Fredonia' and 'Niagara') and a seedling of a complex hybrid of 'Melody' (='Melody Seedling') with the gene Agl-iaaM (Agl = flower specific and developing fruit specific promoter; iaaM = indoleacetamide monooxygenase gene), which should induce seedlessness in these grape cultivars. To achieve this goal, three experiments were designed. In the first experiment, a method for the establishment and maintenance, as well as the regeneration and conversion of grapevine embryogenic cultures was established. In the second experiment, an efficient transformation method for 'Fredonia' and 'Melody Seedling' grapes, based on an Agrobacterium tumefaciens vector system, was developed. Finally, in the third experiment, 'Melody Seedling' was successfully transformed with the Agl5-iaaM gene. This is the first report of successful embryogenesis and genetic transformation with recovery of transgenic plants in V. x labruscana and 'Melody Seedling'. The techniques developed in this research will facilitate the genetic manipulation of the American grapes (V. x labruscana) and open a new scope to study and understand seedlessness in grapevines.","abstract_html":"The main goal of this research was to transform Vitis. x labruscana L. H. Bailey ('Fredonia' and 'Niagara') and a seedling of a complex hybrid of 'Melody' (='Melody Seedling') with the gene Agl-iaaM (Agl = flower specific and developing fruit specific promoter; iaaM = indoleacetamide monooxygenase gene), which should induce seedlessness in these grape cultivars. To achieve this goal, three experiments were designed. In the first experiment, a method for the establishment and maintenance, as well as the regeneration and conversion of grapevine embryogenic cultures was established. In the second experiment, an efficient transformation method for 'Fredonia' and 'Melody Seedling' grapes, based on an Agrobacterium tumefaciens vector system, was developed. Finally, in the third experiment, 'Melody Seedling' was successfully transformed with the Agl5-iaaM gene. This is the first report of successful embryogenesis and genetic transformation with recovery of transgenic plants in V. x labruscana and 'Melody Seedling'. The techniques developed in this research will facilitate the genetic manipulation of the American grapes (V. x labruscana) and open a new scope to study and understand seedlessness in grapevines.","abstract_has_math":false,"creators":["Motoike, Sergio Yoshimitsu"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Natural Resrouces and Environmental Sciences","degree_department":null,"school":null,"contributors":["Skirvin, Robert M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T21:00:17Z","date_published":"2015-09-25T21:00:17Z","updated_at":"2026-07-22T22:26:20Z","subjects":["Agriculture, Plant Culture"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3023147"],"render_values":[{"text":"(MiAaPQ)AAI3023147","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/83077","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Skirvin, Robert M."]},{"key":"dc:creator","label":"Author","values":["Motoike, Sergio Yoshimitsu"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T21:00:17Z","10000-01-01","2001"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Natural Resrouces and Environmental Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Plant Culture"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/83077","(MiAaPQ)AAI3023147"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The main goal of this research was to transform Vitis. x labruscana L. H. Bailey ('Fredonia' and 'Niagara') and a seedling of a complex hybrid of 'Melody' (='Melody Seedling') with the gene Agl-iaaM (Agl = flower specific and developing fruit specific promoter; iaaM = indoleacetamide monooxygenase gene), which should induce seedlessness in these grape cultivars. To achieve this goal, three experiments were designed. In the first experiment, a method for the establishment and maintenance, as well as the regeneration and conversion of grapevine embryogenic cultures was established. In the second experiment, an efficient transformation method for 'Fredonia' and 'Melody Seedling' grapes, based on an Agrobacterium tumefaciens vector system, was developed. Finally, in the third experiment, 'Melody Seedling' was successfully transformed with the Agl5-iaaM gene. This is the first report of successful embryogenesis and genetic transformation with recovery of transgenic plants in V. x labruscana and 'Melody Seedling'. The techniques developed in this research will facilitate the genetic manipulation of the American grapes (V. x labruscana) and open a new scope to study and understand seedlessness in grapevines.","Made available in DSpace on 2015-09-25T21:00:17Z (GMT). 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Bailey ('Fredonia' and 'Niagara') and a seedling of a complex hybrid of 'Melody' (='Melody Seedling') with the gene Agl-iaaM (Agl = flower specific and developing fruit specific promoter; iaaM = indoleacetamide monooxygenase gene), which should induce seedlessness in these grape cultivars. To achieve this goal, three experiments were designed. In the first experiment, a method for the establishment and maintenance, as well as the regeneration and conversion of grapevine embryogenic cultures was established. In the second experiment, an efficient transformation method for 'Fredonia' and 'Melody Seedling' grapes, based on an Agrobacterium tumefaciens vector system, was developed. Finally, in the third experiment, 'Melody Seedling' was successfully transformed with the Agl5-iaaM gene. This is the first report of successful embryogenesis and genetic transformation with recovery of transgenic plants in V. x labruscana and 'Melody Seedling'. The techniques developed in this research will facilitate the genetic manipulation of the American grapes (V. x labruscana) and open a new scope to study and understand seedlessness in grapevines.","Made available in DSpace on 2015-09-25T21:00:17Z (GMT). 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