{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/82759"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/82759","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Structural Study of Natural and Synthetic Polymers","abstract":"In the second part, the molecular composition, size and conformation of zein, corn prolamines, was examined with MALDI-MS, TEM, SEC3, SAXS and viscometry. MALDI-MS is demonstrated to be a superior technique to study the molecular weight and composition of zein proteins than conventional SDS-PAGE. The observed large particle size from TEM and SEC3 compared to the predicted value from the molecular weight suggested that zein tends to form oligomeric structures. The denaturation behavior of zero was also studied using various denaturants. The results showed most denaturation agents, such as pH, guanidine hydrochloride and DTT, didn't denature zein. Temperature and SDS may cause some denaturation as suggested from the increase of the intrinsic viscosity; the change of intrinsic viscosity was not sufficient to indicate coil-like conformation.","abstract_html":"In the second part, the molecular composition, size and conformation of zein, corn prolamines, was examined with MALDI-MS, TEM, SEC3, SAXS and viscometry. MALDI-MS is demonstrated to be a superior technique to study the molecular weight and composition of zein proteins than conventional SDS-PAGE. The observed large particle size from TEM and SEC3 compared to the predicted value from the molecular weight suggested that zein tends to form oligomeric structures. The denaturation behavior of zero was also studied using various denaturants. The results showed most denaturation agents, such as pH, guanidine hydrochloride and DTT, didn't denature zein. Temperature and SDS may cause some denaturation as suggested from the increase of the intrinsic viscosity; the change of intrinsic viscosity was not sufficient to indicate coil-like conformation.","abstract_has_math":false,"creators":["Wang, Jinfeng"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Materials Science and Engineering","degree_department":null,"school":null,"contributors":["Geil, Phillip H."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T20:52:54Z","date_published":"2015-09-25T20:52:54Z","updated_at":"2026-07-22T22:26:18Z","subjects":["Chemistry, Polymer"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3160966"],"render_values":[{"text":"(MiAaPQ)AAI3160966","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/82759","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Geil, Phillip H."]},{"key":"dc:creator","label":"Author","values":["Wang, Jinfeng"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T20:52:54Z","10000-01-01","2004"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Materials Science and Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Polymer"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/82759","(MiAaPQ)AAI3160966"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["In the second part, the molecular composition, size and conformation of zein, corn prolamines, was examined with MALDI-MS, TEM, SEC3, SAXS and viscometry. MALDI-MS is demonstrated to be a superior technique to study the molecular weight and composition of zein proteins than conventional SDS-PAGE. The observed large particle size from TEM and SEC3 compared to the predicted value from the molecular weight suggested that zein tends to form oligomeric structures. The denaturation behavior of zero was also studied using various denaturants. The results showed most denaturation agents, such as pH, guanidine hydrochloride and DTT, didn't denature zein. Temperature and SDS may cause some denaturation as suggested from the increase of the intrinsic viscosity; the change of intrinsic viscosity was not sufficient to indicate coil-like conformation.","Made available in DSpace on 2015-09-25T20:52:54Z (GMT). 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MALDI-MS is demonstrated to be a superior technique to study the molecular weight and composition of zein proteins than conventional SDS-PAGE. The observed large particle size from TEM and SEC3 compared to the predicted value from the molecular weight suggested that zein tends to form oligomeric structures. The denaturation behavior of zero was also studied using various denaturants. The results showed most denaturation agents, such as pH, guanidine hydrochloride and DTT, didn't denature zein. Temperature and SDS may cause some denaturation as suggested from the increase of the intrinsic viscosity; the change of intrinsic viscosity was not sufficient to indicate coil-like conformation.","Made available in DSpace on 2015-09-25T20:52:54Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3160966.pdf: 13569294 bytes, checksum: 375db0d69002c86841d9abb883ace5a3 (MD5) Previous issue date: 2004","Embargo set by: Seth Robbins for item 84040 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","147 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2004."],"dc:identifier":["http://hdl.handle.net/2142/82759","(MiAaPQ)AAI3160966"],"dc:language":["eng"],"dc:subject":["Chemistry, Polymer"],"dc:title":["Structural Study of Natural and Synthetic Polymers"],"dc:type":["text"],"thesis:degree_discipline":["Materials Science and Engineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:18Z"}