{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/82493"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/82493","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Identification and Characterization of the Neurotrophic Properties of Dopamine -Releasing Protein (Darp)","abstract":"In a third set of experiments, I assessed the effects of DARP-36aa on the survival of mesencephalic neurons in vitro. Treatment of mesencephalic cultures with DARP36aa resulted in a significant increase in neuron survival and morphological development. Mesencephalic cultures were also incubated with antibodies against DARP-36aa (antiDARP-36aa) in order to assess the effect of immunoneutralization of endogenous DARP on these cells. Mesencephalic cultures treated with anti-DARP-36aa contained significantly fewer neurons, and the number of branching points per neuron was markedly decreased compared to controls. DARP-36aa treatments significantly increased the number of catecholaminergic neurons in mesencephalic cultures while immunoneutralization of endogenous DARP significantly decreased catecholaminergic neuron number and morphological development. Importantly, DARP-36aa-mediated increases in neuron survival and development were completely attenuated by coincubation with genistein. In conclusion, findings from the aforementioned studies suggest that DARP-36aa is a novel neurotrophic factor that promotes survival and development of mesencephalic neurons.","abstract_html":"In a third set of experiments, I assessed the effects of DARP-36aa on the survival of mesencephalic neurons in vitro. Treatment of mesencephalic cultures with DARP36aa resulted in a significant increase in neuron survival and morphological development. Mesencephalic cultures were also incubated with antibodies against DARP-36aa (antiDARP-36aa) in order to assess the effect of immunoneutralization of endogenous DARP on these cells. Mesencephalic cultures treated with anti-DARP-36aa contained significantly fewer neurons, and the number of branching points per neuron was markedly decreased compared to controls. DARP-36aa treatments significantly increased the number of catecholaminergic neurons in mesencephalic cultures while immunoneutralization of endogenous DARP significantly decreased catecholaminergic neuron number and morphological development. Importantly, DARP-36aa-mediated increases in neuron survival and development were completely attenuated by coincubation with genistein. In conclusion, findings from the aforementioned studies suggest that DARP-36aa is a novel neurotrophic factor that promotes survival and development of mesencephalic neurons.","abstract_has_math":false,"creators":["Smith, Sean Michael"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Neuroscience","degree_department":null,"school":null,"contributors":["Ramirez, Victor D."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T20:45:32Z","date_published":"2015-09-25T20:45:32Z","updated_at":"2026-07-22T22:26:18Z","subjects":["Biology, Neuroscience"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3070441"],"render_values":[{"text":"(MiAaPQ)AAI3070441","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/82493","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Ramirez, Victor D."]},{"key":"dc:creator","label":"Author","values":["Smith, Sean Michael"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T20:45:32Z","10000-01-01","2002"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Neuroscience"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Neuroscience"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/82493","(MiAaPQ)AAI3070441"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["In a third set of experiments, I assessed the effects of DARP-36aa on the survival of mesencephalic neurons in vitro. Treatment of mesencephalic cultures with DARP36aa resulted in a significant increase in neuron survival and morphological development. Mesencephalic cultures were also incubated with antibodies against DARP-36aa (antiDARP-36aa) in order to assess the effect of immunoneutralization of endogenous DARP on these cells. Mesencephalic cultures treated with anti-DARP-36aa contained significantly fewer neurons, and the number of branching points per neuron was markedly decreased compared to controls. DARP-36aa treatments significantly increased the number of catecholaminergic neurons in mesencephalic cultures while immunoneutralization of endogenous DARP significantly decreased catecholaminergic neuron number and morphological development. Importantly, DARP-36aa-mediated increases in neuron survival and development were completely attenuated by coincubation with genistein. In conclusion, findings from the aforementioned studies suggest that DARP-36aa is a novel neurotrophic factor that promotes survival and development of mesencephalic neurons.","Made available in DSpace on 2015-09-25T20:45:32Z (GMT). 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Treatment of mesencephalic cultures with DARP36aa resulted in a significant increase in neuron survival and morphological development. Mesencephalic cultures were also incubated with antibodies against DARP-36aa (antiDARP-36aa) in order to assess the effect of immunoneutralization of endogenous DARP on these cells. Mesencephalic cultures treated with anti-DARP-36aa contained significantly fewer neurons, and the number of branching points per neuron was markedly decreased compared to controls. DARP-36aa treatments significantly increased the number of catecholaminergic neurons in mesencephalic cultures while immunoneutralization of endogenous DARP significantly decreased catecholaminergic neuron number and morphological development. Importantly, DARP-36aa-mediated increases in neuron survival and development were completely attenuated by coincubation with genistein. 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