{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/82352"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/82352","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Engineering of Nonviral Vectors for Gene Therapy","abstract":"Finally, for polymeric devices to be made safe and efficient for in vivo usage there must be some means to target them to specific cells or tissues. Various cyclodextrins were conjugated to PEI using an amide linkage. The hydrophobic interior of the cyclodextrin allows the facile addition of small targeting and lysomotropic agents to the polyplex without chemical conjugation. By applying a hydrophobic analog of human insulin to the cyclodextrin polyplexes, I was able demonstrate a 2--3 fold enhancement in transfection efficiency in insulin receptor-rich cell lines.","abstract_html":"Finally, for polymeric devices to be made safe and efficient for in vivo usage there must be some means to target them to specific cells or tissues. Various cyclodextrins were conjugated to PEI using an amide linkage. The hydrophobic interior of the cyclodextrin allows the facile addition of small targeting and lysomotropic agents to the polyplex without chemical conjugation. By applying a hydrophobic analog of human insulin to the cyclodextrin polyplexes, I was able demonstrate a 2--3 fold enhancement in transfection efficiency in insulin receptor-rich cell lines.","abstract_has_math":false,"creators":["Forrest, Marcus Laird"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemical and Biomolecular Engineering","degree_department":null,"school":null,"contributors":["Pack, Daniel W."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T20:43:16Z","date_published":"2015-09-25T20:43:16Z","updated_at":"2026-07-22T22:26:18Z","subjects":["Engineering, Biomedical"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3101840"],"render_values":[{"text":"(MiAaPQ)AAI3101840","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/82352","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Pack, Daniel W."]},{"key":"dc:creator","label":"Author","values":["Forrest, Marcus Laird"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T20:43:16Z","10000-01-01","2003"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemical and Biomolecular Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Engineering, Biomedical"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/82352","(MiAaPQ)AAI3101840"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Finally, for polymeric devices to be made safe and efficient for in vivo usage there must be some means to target them to specific cells or tissues. Various cyclodextrins were conjugated to PEI using an amide linkage. The hydrophobic interior of the cyclodextrin allows the facile addition of small targeting and lysomotropic agents to the polyplex without chemical conjugation. By applying a hydrophobic analog of human insulin to the cyclodextrin polyplexes, I was able demonstrate a 2--3 fold enhancement in transfection efficiency in insulin receptor-rich cell lines.","Made available in DSpace on 2015-09-25T20:43:16Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3101840.pdf: 6385399 bytes, checksum: 48f8464ed3a6a738108dc7a1988bd0f8 (MD5) Previous issue date: 2003","Embargo set by: Seth Robbins for item 83633 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","150 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2003."]},{"key":"dc:title","label":"Title","values":["Engineering of Nonviral Vectors for Gene Therapy"]}]}],"canonical_facts":{"dc:contributor":["Pack, Daniel W."],"dc:creator":["Forrest, Marcus Laird"],"dc:date":["2015-09-25T20:43:16Z","10000-01-01","2003"],"dc:description":["Finally, for polymeric devices to be made safe and efficient for in vivo usage there must be some means to target them to specific cells or tissues. Various cyclodextrins were conjugated to PEI using an amide linkage. The hydrophobic interior of the cyclodextrin allows the facile addition of small targeting and lysomotropic agents to the polyplex without chemical conjugation. By applying a hydrophobic analog of human insulin to the cyclodextrin polyplexes, I was able demonstrate a 2--3 fold enhancement in transfection efficiency in insulin receptor-rich cell lines.","Made available in DSpace on 2015-09-25T20:43:16Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3101840.pdf: 6385399 bytes, checksum: 48f8464ed3a6a738108dc7a1988bd0f8 (MD5) Previous issue date: 2003","Embargo set by: Seth Robbins for item 83633 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","150 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2003."],"dc:identifier":["http://hdl.handle.net/2142/82352","(MiAaPQ)AAI3101840"],"dc:language":["eng"],"dc:subject":["Engineering, Biomedical"],"dc:title":["Engineering of Nonviral Vectors for Gene Therapy"],"dc:type":["text"],"thesis:degree_discipline":["Chemical and Biomolecular Engineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:18Z"}