{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/78791"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/78791","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Photochemically produced reactive species generation by extracellular organic matter sensitizers from Chlamydomonas reinhardtii","abstract":"Microalgae biotechnologies have shown promise as tertiary wastewater treatment processes capable of reducing nitrogen and phosphorous to meet increasingly stringent regulatory limits and as producers of biofuel feedstock. During growth and respiration, microalgae excrete extracellular organic matter (EOM) as metabolic byproducts which have the potential to act as photosensitizers for the generation of reactive species, including singlet oxygen (1O2), hydroxyl radicals (•OH), and triplet excited dissolved organic matter (3DOM). These reactive species can play an essential role in the mineralization of dissolved organic matter, nutrient cycling and bioavailability, attenuation of toxic pollutants, and inactivation of pathogens. Recent studies observed enhanced transformation of organic micropollutants in UV and visible light irradiated algae suspensions where photogenerated 1O2 and •OH were detected. The photochemistry in EOM matrices is still largely unknown as well as the impacts of reactive species on cultivation including EOM mineralization, protective extracellular reactive species generation, and nutrient availability. This study reports on reactive species production in EOM solutions separated from pure batch cultures of Chlamydomonas reinhardtii under solar irradiation. Results show increasing steady-state levels of 3DOM, 1O2, and formation rates of •OH under sunlight irradiation as EOM levels increase with batch culture growth. Changes in reactive species levels were compared with changes in culture characteristics such as volatile suspended solids (VSS) and nutrient availability as well as EOM properties including dissolved organic carbon (DOC) and specific UV absorbance (SUVA). EOM-sensitized reactive species photogeneration in comparison to other DOM sources was also discussed along with implications for the fate of contaminants, EOM, and culture stability in photobioreactors.","abstract_html":"Microalgae biotechnologies have shown promise as tertiary wastewater treatment processes capable of reducing nitrogen and phosphorous to meet increasingly stringent regulatory limits and as producers of biofuel feedstock. During growth and respiration, microalgae excrete extracellular organic matter (EOM) as metabolic byproducts which have the potential to act as photosensitizers for the generation of reactive species, including singlet oxygen (1O2), hydroxyl radicals (•OH), and triplet excited dissolved organic matter (3DOM). These reactive species can play an essential role in the mineralization of dissolved organic matter, nutrient cycling and bioavailability, attenuation of toxic pollutants, and inactivation of pathogens. Recent studies observed enhanced transformation of organic micropollutants in UV and visible light irradiated algae suspensions where photogenerated 1O2 and •OH were detected. The photochemistry in EOM matrices is still largely unknown as well as the impacts of reactive species on cultivation including EOM mineralization, protective extracellular reactive species generation, and nutrient availability. This study reports on reactive species production in EOM solutions separated from pure batch cultures of Chlamydomonas reinhardtii under solar irradiation. Results show increasing steady-state levels of 3DOM, 1O2, and formation rates of •OH under sunlight irradiation as EOM levels increase with batch culture growth. Changes in reactive species levels were compared with changes in culture characteristics such as volatile suspended solids (VSS) and nutrient availability as well as EOM properties including dissolved organic carbon (DOC) and specific UV absorbance (SUVA). EOM-sensitized reactive species photogeneration in comparison to other DOM sources was also discussed along with implications for the fate of contaminants, EOM, and culture stability in photobioreactors.","abstract_has_math":false,"creators":["Tenorio, Raul"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Environ Engr in Civil Engr","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-07-22T22:46:05Z","date_published":"2015-07-22T22:46:05Z","updated_at":"2026-07-22T22:26:12Z","subjects":["Reactive oxygen species","algae cultivation","algal organic matter"],"languages":["en"],"rights":["Copyright 2015 Raul Tenorio"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/78791","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Tenorio, Raul"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-07-22T22:46:05Z","2017-07-23T09:15:17Z","2015-05","2015-04-30","2015-5"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Environ Engr in Civil Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Reactive oxygen species","algae cultivation","algal organic matter"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2015 Raul Tenorio"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/78791"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Microalgae biotechnologies have shown promise as tertiary wastewater treatment processes capable of reducing nitrogen and phosphorous to meet increasingly stringent regulatory limits and as producers of biofuel feedstock. During growth and respiration, microalgae excrete extracellular organic matter (EOM) as metabolic byproducts which have the potential to act as photosensitizers for the generation of reactive species, including singlet oxygen (1O2), hydroxyl radicals (•OH), and triplet excited dissolved organic matter (3DOM). These reactive species can play an essential role in the mineralization of dissolved organic matter, nutrient cycling and bioavailability, attenuation of toxic pollutants, and inactivation of pathogens. Recent studies observed enhanced transformation of organic micropollutants in UV and visible light irradiated algae suspensions where photogenerated 1O2 and •OH were detected. The photochemistry in EOM matrices is still largely unknown as well as the impacts of reactive species on cultivation including EOM mineralization, protective extracellular reactive species generation, and nutrient availability. This study reports on reactive species production in EOM solutions separated from pure batch cultures of Chlamydomonas reinhardtii under solar irradiation. Results show increasing steady-state levels of 3DOM, 1O2, and formation rates of •OH under sunlight irradiation as EOM levels increase with batch culture growth. Changes in reactive species levels were compared with changes in culture characteristics such as volatile suspended solids (VSS) and nutrient availability as well as EOM properties including dissolved organic carbon (DOC) and specific UV absorbance (SUVA). EOM-sensitized reactive species photogeneration in comparison to other DOM sources was also discussed along with implications for the fate of contaminants, EOM, and culture stability in photobioreactors.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2017-05-01","The student, Raul Tenorio, accepted the attached license on 2015-04-28 at 10:36.","The student, Raul Tenorio, submitted this Thesis for approval on 2015-04-28 at 10:42.","This Thesis was approved for publication on 2015-04-30 at 13:56.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8143 on 2015-07-22 at 14:26:32","Made available in DSpace on 2015-07-22T22:46:05Z (GMT). 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During growth and respiration, microalgae excrete extracellular organic matter (EOM) as metabolic byproducts which have the potential to act as photosensitizers for the generation of reactive species, including singlet oxygen (1O2), hydroxyl radicals (•OH), and triplet excited dissolved organic matter (3DOM). These reactive species can play an essential role in the mineralization of dissolved organic matter, nutrient cycling and bioavailability, attenuation of toxic pollutants, and inactivation of pathogens. Recent studies observed enhanced transformation of organic micropollutants in UV and visible light irradiated algae suspensions where photogenerated 1O2 and •OH were detected. The photochemistry in EOM matrices is still largely unknown as well as the impacts of reactive species on cultivation including EOM mineralization, protective extracellular reactive species generation, and nutrient availability. This study reports on reactive species production in EOM solutions separated from pure batch cultures of Chlamydomonas reinhardtii under solar irradiation. Results show increasing steady-state levels of 3DOM, 1O2, and formation rates of •OH under sunlight irradiation as EOM levels increase with batch culture growth. Changes in reactive species levels were compared with changes in culture characteristics such as volatile suspended solids (VSS) and nutrient availability as well as EOM properties including dissolved organic carbon (DOC) and specific UV absorbance (SUVA). EOM-sensitized reactive species photogeneration in comparison to other DOM sources was also discussed along with implications for the fate of contaminants, EOM, and culture stability in photobioreactors.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2017-05-01","The student, Raul Tenorio, accepted the attached license on 2015-04-28 at 10:36.","The student, Raul Tenorio, submitted this Thesis for approval on 2015-04-28 at 10:42.","This Thesis was approved for publication on 2015-04-30 at 13:56.","DSpace SAF Submission Ingestion Package generated from Vireo submission #8143 on 2015-07-22 at 14:26:32","Made available in DSpace on 2015-07-22T22:46:05Z (GMT). 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