{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/78590"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/78590","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"External cavity laser biosensor for label-free detection","abstract":"Optical label-free biosensors serve as a powerful detection tool to analyze biomolecular interactions, and have been widely used in pharmaceutical drug discovery and biochemical sensing. Desirable performance metrics for such sensors include high sensitivity and fine resolution, which are essential for achieving high-precision detection of small molecules. In this work, we demonstrate a dual-mode external cavity laser (ECL) biosensor to address the desired needs. The utilization of photonic crystal resonant reflector biosensor as mode-selection filter enables high sensitivity, while the stimulated emission process in the laser cavity provides high spectral resolution. Additionally, dual-mode operation of the ECL system enables a self- referencing technique which significantly improves the signal to noise ratio. This advanced instrument enables direct detection of small molecule binding to immobilized protein targets, which is essential for drug discovery process. We develop a plasmonic ECL to further improve the performance metrics of ECL biosensors. Utilizing surface plasmon resonance (SPR) biosensor as mode-selection filter, the plasmonic ECL demonstrates 3× higher sensitivity than photonic crystal based ECL biosensor. Moreover, compared to that of traditional SPR biosensors, the quality factor of the plasmonic laser biosensor is significantly enhanced, improving the spectral resolution. The enhanced biosensing capability of the plasmonic ECL offers unique opportunity for detecting biologically important molecules and viral particles in extremely low concentration.","abstract_html":"Optical label-free biosensors serve as a powerful detection tool to analyze biomolecular interactions, and have been widely used in pharmaceutical drug discovery and biochemical sensing. Desirable performance metrics for such sensors include high sensitivity and fine resolution, which are essential for achieving high-precision detection of small molecules. In this work, we demonstrate a dual-mode external cavity laser (ECL) biosensor to address the desired needs. The utilization of photonic crystal resonant reflector biosensor as mode-selection filter enables high sensitivity, while the stimulated emission process in the laser cavity provides high spectral resolution. Additionally, dual-mode operation of the ECL system enables a self- referencing technique which significantly improves the signal to noise ratio. This advanced instrument enables direct detection of small molecule binding to immobilized protein targets, which is essential for drug discovery process. We develop a plasmonic ECL to further improve the performance metrics of ECL biosensors. Utilizing surface plasmon resonance (SPR) biosensor as mode-selection filter, the plasmonic ECL demonstrates 3× higher sensitivity than photonic crystal based ECL biosensor. Moreover, compared to that of traditional SPR biosensors, the quality factor of the plasmonic laser biosensor is significantly enhanced, improving the spectral resolution. The enhanced biosensing capability of the plasmonic ECL offers unique opportunity for detecting biologically important molecules and viral particles in extremely low concentration.","abstract_has_math":false,"creators":["Zhang, Meng"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Physics","degree_department":null,"school":null,"contributors":["Cunningham, Brian T.","Cooper, Lance","Eden, Gary J.","Hergenrother, Paul J."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-07-22T22:32:55Z","date_published":"2015-07-22T22:32:55Z","updated_at":"2026-07-22T22:26:12Z","subjects":["optical biosensor","tunable laser"],"languages":["en"],"rights":["Copyright 2015 Meng Zhang"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/78590","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Cunningham, Brian T.","Cooper, Lance","Eden, Gary J.","Hergenrother, Paul J."]},{"key":"dc:creator","label":"Author","values":["Zhang, Meng"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-07-22T22:32:55Z","2017-07-23T09:15:31Z","2015-05","2015-04-16","2015-5"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physics"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["optical biosensor","tunable laser"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2015 Meng Zhang"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/78590"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Optical label-free biosensors serve as a powerful detection tool to analyze biomolecular interactions, and have been widely used in pharmaceutical drug discovery and biochemical sensing. Desirable performance metrics for such sensors include high sensitivity and fine resolution, which are essential for achieving high-precision detection of small molecules. In this work, we demonstrate a dual-mode external cavity laser (ECL) biosensor to address the desired needs. The utilization of photonic crystal resonant reflector biosensor as mode-selection filter enables high sensitivity, while the stimulated emission process in the laser cavity provides high spectral resolution. Additionally, dual-mode operation of the ECL system enables a self- referencing technique which significantly improves the signal to noise ratio. This advanced instrument enables direct detection of small molecule binding to immobilized protein targets, which is essential for drug discovery process. We develop a plasmonic ECL to further improve the performance metrics of ECL biosensors. Utilizing surface plasmon resonance (SPR) biosensor as mode-selection filter, the plasmonic ECL demonstrates 3× higher sensitivity than photonic crystal based ECL biosensor. Moreover, compared to that of traditional SPR biosensors, the quality factor of the plasmonic laser biosensor is significantly enhanced, improving the spectral resolution. The enhanced biosensing capability of the plasmonic ECL offers unique opportunity for detecting biologically important molecules and viral particles in extremely low concentration.","Submission published under a 24 month embargo labeled 'U of I only', the embargo will last until 2017-05-01","The student, Meng Zhang, accepted the attached license on 2015-03-20 at 11:34.","The student, Meng Zhang, submitted this Dissertation for approval on 2015-03-20 at 11:39.","This Dissertation was approved for publication on 2015-04-16 at 12:12.","DSpace SAF Submission Ingestion Package generated from Vireo submission #7767 on 2015-07-22 at 14:16:51","Made available in DSpace on 2015-07-22T22:32:55Z (GMT). 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Desirable performance metrics for such sensors include high sensitivity and fine resolution, which are essential for achieving high-precision detection of small molecules. In this work, we demonstrate a dual-mode external cavity laser (ECL) biosensor to address the desired needs. The utilization of photonic crystal resonant reflector biosensor as mode-selection filter enables high sensitivity, while the stimulated emission process in the laser cavity provides high spectral resolution. Additionally, dual-mode operation of the ECL system enables a self- referencing technique which significantly improves the signal to noise ratio. This advanced instrument enables direct detection of small molecule binding to immobilized protein targets, which is essential for drug discovery process. We develop a plasmonic ECL to further improve the performance metrics of ECL biosensors. Utilizing surface plasmon resonance (SPR) biosensor as mode-selection filter, the plasmonic ECL demonstrates 3× higher sensitivity than photonic crystal based ECL biosensor. Moreover, compared to that of traditional SPR biosensors, the quality factor of the plasmonic laser biosensor is significantly enhanced, improving the spectral resolution. The enhanced biosensing capability of the plasmonic ECL offers unique opportunity for detecting biologically important molecules and viral particles in extremely low concentration.","Submission published under a 24 month embargo labeled 'U of I only', the embargo will last until 2017-05-01","The student, Meng Zhang, accepted the attached license on 2015-03-20 at 11:34.","The student, Meng Zhang, submitted this Dissertation for approval on 2015-03-20 at 11:39.","This Dissertation was approved for publication on 2015-04-16 at 12:12.","DSpace SAF Submission Ingestion Package generated from Vireo submission #7767 on 2015-07-22 at 14:16:51","Made available in DSpace on 2015-07-22T22:32:55Z (GMT). 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