{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/78577"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/78577","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Lineage specification of ovarian theca cells requires multi-cellular interactions via oocyte and granulosa cells","abstract":"Organogenesis of the ovary is a highly orchestrated process involving multiple lineage determinations of ovarian surface epithelium, granulosa cells, and theca cells. While the sources of ovarian surface epithelium and granulosa cells are known, the embryonic origin(s) of theca stem/progenitor cells have not been definitively identified. Here I show that theca cells derive from two sources: Wt1-positive cells indigenous to the ovary and Gli1-positive mesenchymal cells that migrate from the mesonephros. These two theca progenitor populations are distinct in their cell lineage contributions, relative proximity to granulosa cells, and their gene expression profiles. The two sources of theca progenitors acquire theca lineage marker Gli1 in the ovary soon after birth, in response to paracrine signals Desert hedgehog (Dhh) and Indian hedgehog (Ihh) from granulosa cells. Ovaries lacking Dhh/Ihh exhibited a loss of theca layer, blunted steroid production, arrested folliculogenesis, and failure to form corpora lutea. Production of Dhh/Ihh in granulosa cells requires growth differentiation factor 9 (GDF9) from the oocyte. Gdf9 ablation resulted in diminished expression of Dhh, Ihh, and theca cell-specific Gli1. Conversely, supplementation of GDF9 to oocyte-depleted ovaries reactivated Dhh/Ihh production and subsequent expression of Gli1. My studies provide the first genetic evidence for the origins of theca cells and reveal a novel multicellular interaction via GDF9 and Hedgehog signaling critical for ovarian folliculogenesis and formation of a functional theca.","abstract_html":"Organogenesis of the ovary is a highly orchestrated process involving multiple lineage determinations of ovarian surface epithelium, granulosa cells, and theca cells. While the sources of ovarian surface epithelium and granulosa cells are known, the embryonic origin(s) of theca stem/progenitor cells have not been definitively identified. Here I show that theca cells derive from two sources: Wt1-positive cells indigenous to the ovary and Gli1-positive mesenchymal cells that migrate from the mesonephros. These two theca progenitor populations are distinct in their cell lineage contributions, relative proximity to granulosa cells, and their gene expression profiles. The two sources of theca progenitors acquire theca lineage marker Gli1 in the ovary soon after birth, in response to paracrine signals Desert hedgehog (Dhh) and Indian hedgehog (Ihh) from granulosa cells. Ovaries lacking Dhh/Ihh exhibited a loss of theca layer, blunted steroid production, arrested folliculogenesis, and failure to form corpora lutea. Production of Dhh/Ihh in granulosa cells requires growth differentiation factor 9 (GDF9) from the oocyte. Gdf9 ablation resulted in diminished expression of Dhh, Ihh, and theca cell-specific Gli1. Conversely, supplementation of GDF9 to oocyte-depleted ovaries reactivated Dhh/Ihh production and subsequent expression of Gli1. My studies provide the first genetic evidence for the origins of theca cells and reveal a novel multicellular interaction via GDF9 and Hedgehog signaling critical for ovarian folliculogenesis and formation of a functional theca.","abstract_has_math":false,"creators":["Liu, Chang"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Animal Sciences","degree_department":null,"school":null,"contributors":["Yao, Humphrey H-C.","Bahr, Janice M.","Miller, David J.","Nowak, Romana A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-05","date_published":"2015-05","updated_at":"2026-07-22T22:26:12Z","subjects":["Hedgehog","growth differentiation factor 9 (GDF9)","Theca cell","Lineage specification"],"languages":[],"rights":["Copyright 2015 Chang Liu"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/78577","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Yao, Humphrey H-C.","Bahr, Janice M.","Miller, David J.","Nowak, Romana A."]},{"key":"dc:creator","label":"Author","values":["Liu, Chang"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-05","2015-07-22T22:32:37Z","2017-07-23T09:15:31Z","2014-12-03"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Hedgehog","growth differentiation factor 9 (GDF9)","Theca cell","Lineage specification"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2015 Chang Liu"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/78577"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Organogenesis of the ovary is a highly orchestrated process involving multiple lineage determinations of ovarian surface epithelium, granulosa cells, and theca cells. While the sources of ovarian surface epithelium and granulosa cells are known, the embryonic origin(s) of theca stem/progenitor cells have not been definitively identified. Here I show that theca cells derive from two sources: Wt1-positive cells indigenous to the ovary and Gli1-positive mesenchymal cells that migrate from the mesonephros. These two theca progenitor populations are distinct in their cell lineage contributions, relative proximity to granulosa cells, and their gene expression profiles. The two sources of theca progenitors acquire theca lineage marker Gli1 in the ovary soon after birth, in response to paracrine signals Desert hedgehog (Dhh) and Indian hedgehog (Ihh) from granulosa cells. Ovaries lacking Dhh/Ihh exhibited a loss of theca layer, blunted steroid production, arrested folliculogenesis, and failure to form corpora lutea. Production of Dhh/Ihh in granulosa cells requires growth differentiation factor 9 (GDF9) from the oocyte. Gdf9 ablation resulted in diminished expression of Dhh, Ihh, and theca cell-specific Gli1. Conversely, supplementation of GDF9 to oocyte-depleted ovaries reactivated Dhh/Ihh production and subsequent expression of Gli1. My studies provide the first genetic evidence for the origins of theca cells and reveal a novel multicellular interaction via GDF9 and Hedgehog signaling critical for ovarian folliculogenesis and formation of a functional theca.","Submission published under a 24 month embargo labeled 'U of I only', the embargo will last until 2017-05-01","The student, Chang Liu, accepted the attached license on 2014-12-02 at 09:50.","The student, Chang Liu, submitted this Dissertation for approval on 2014-12-02 at 10:00.","This Dissertation was approved for publication on 2014-12-03 at 09:48.","DSpace SAF Submission Ingestion Package generated from Vireo submission #7489 on 2015-07-22 at 14:16:26","Made available in DSpace on 2015-07-22T22:32:37Z (GMT). No. of bitstreams: 2 Liu_Chang1.pdf: 6658420 bytes, checksum: 3a6651577160cb5b4722053432054e7c (MD5) license.txt: 4057 bytes, checksum: 35b8d6be9410ac1e0b54a45f6dacdebd (MD5) Previous issue date: 2014-12-03","Embargo set by: Seth Robbins for item 79818 Lift date: 2017-07-22T22:34:16Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only Restriction Lifted for Item 79818 on 2017-07-23T09:15:31Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Lineage specification of ovarian theca cells requires multi-cellular interactions via oocyte and granulosa cells"]}]}],"canonical_facts":{"dc:contributor":["Yao, Humphrey H-C.","Bahr, Janice M.","Miller, David J.","Nowak, Romana A."],"dc:creator":["Liu, Chang"],"dc:date":["2015-05","2015-07-22T22:32:37Z","2017-07-23T09:15:31Z","2014-12-03"],"dc:description":["Organogenesis of the ovary is a highly orchestrated process involving multiple lineage determinations of ovarian surface epithelium, granulosa cells, and theca cells. While the sources of ovarian surface epithelium and granulosa cells are known, the embryonic origin(s) of theca stem/progenitor cells have not been definitively identified. Here I show that theca cells derive from two sources: Wt1-positive cells indigenous to the ovary and Gli1-positive mesenchymal cells that migrate from the mesonephros. These two theca progenitor populations are distinct in their cell lineage contributions, relative proximity to granulosa cells, and their gene expression profiles. The two sources of theca progenitors acquire theca lineage marker Gli1 in the ovary soon after birth, in response to paracrine signals Desert hedgehog (Dhh) and Indian hedgehog (Ihh) from granulosa cells. Ovaries lacking Dhh/Ihh exhibited a loss of theca layer, blunted steroid production, arrested folliculogenesis, and failure to form corpora lutea. Production of Dhh/Ihh in granulosa cells requires growth differentiation factor 9 (GDF9) from the oocyte. Gdf9 ablation resulted in diminished expression of Dhh, Ihh, and theca cell-specific Gli1. Conversely, supplementation of GDF9 to oocyte-depleted ovaries reactivated Dhh/Ihh production and subsequent expression of Gli1. My studies provide the first genetic evidence for the origins of theca cells and reveal a novel multicellular interaction via GDF9 and Hedgehog signaling critical for ovarian folliculogenesis and formation of a functional theca.","Submission published under a 24 month embargo labeled 'U of I only', the embargo will last until 2017-05-01","The student, Chang Liu, accepted the attached license on 2014-12-02 at 09:50.","The student, Chang Liu, submitted this Dissertation for approval on 2014-12-02 at 10:00.","This Dissertation was approved for publication on 2014-12-03 at 09:48.","DSpace SAF Submission Ingestion Package generated from Vireo submission #7489 on 2015-07-22 at 14:16:26","Made available in DSpace on 2015-07-22T22:32:37Z (GMT). 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