Microbial phylogenetic diversity preserved in facies-specific modern, recent, Holocene and Pleistocene hot-spring travertine deposits of Yellowstone and Turkey
type: CreativeWork
description: A systematic evaluation has been undertaken of the mechanisms and products of microbial community preservation within modern-to-ancient terrestrial hot-spring calcium carbonate (CaCO3) limestone deposits called travertine. Microbial 16S rRNA gene sequences preserved within Modern travertine deposited within the Proximal Slope Facies (PSF) at Mammoth Hot Springs (MHS), Yellowstone National Park, has been directly compared with analogous Holocene-Late Pleistocene PSF travertine at Gardiner, Montana, and Middle Pleistocene PSF travertine in Denizli, Turkey. Analyses have included: (1) Modern microbial communities inhabiting the PSF of an actively depositing travertine hot-spring system (water, microbial mats, travertine) at MHS (0 YBP Modern travertine, Angel Terrace, Spring AT-1); (2) 9 YBP Modern PSF travertine (Angel Terrace, Spring AT-2; MHS); (2) ~100 YBP Recent travertine (New Highland Terrace, MHS); (3) ~4,000 YBP Holocene travertine (USGS Y-10 Core); (3) ~30,000 YBP Late Pleistocene travertine (Gardiner Quarry); and (4) ~1.1 Ma YBP Middle Pleistocene travertine (all of the Mammoth Hot Springs (YNP) and (Cakmak Quarry, Turkey). Genomic DNA entombed during rapid (up to 5 mm/day) travertine deposition and preserved within CaCO3 fluid inclusions and between crystals, was extracted via bulk rock drilling under sterile clean room conditions. Pooled 16S rRNA gene sequence libraries were constructed via polymerase chain reactions (PCRs), terminal-restriction fragment length polymorphisms (T-RFLP), and MiSeq amplicon sequencing. Blast searches using multiple web-based bioinformatics tools identified over 400 operational taxonomic units (OTUs) affiliated with a total of 19 phyla (16 phyla and 3 candidate phyla) within the Domain Bacteria. Previous analyses of the living microbial communities inhabiting modern active PSF depositional environments at MHS have provided a baseline with which to compare the ancient travertine analyses. Combined results from both MHS and Deniz…
abstract: A systematic evaluation has been undertaken of the mechanisms and products of microbial community preservation within modern-to-ancient terrestrial hot-spring calcium carbonate (CaCO3) limestone deposits called travertine. Microbial 16S rRNA gene sequences preserved within Modern travertine deposited within the Proximal Slope Facies (PSF) at Mammoth Hot Springs (MHS), Yellowstone National Park, has been directly compared with analogous Holocene-Late Pleistocene PSF travertine at Gardiner, Montana, and Middle Pleistocene PSF travertine in Denizli, Turkey. Analyses have included: (1) Modern microbial communities inhabiting the PSF of an actively depositing travertine hot-spring system (water, microbial mats, travertine) at MHS (0 YBP Modern travertine, Angel Terrace, Spring AT-1); (2) 9 YBP Modern PSF travertine (Angel Terrace, Spring AT-2; MHS); (2) ~100 YBP Recent travertine (New Highland Terrace, MHS); (3) ~4,000 YBP Holocene travertine (USGS Y-10 Core); (3) ~30,000 YBP Late Pleistocene travertine (Gardiner Quarry); and (4) ~1.1 Ma YBP Middle Pleistocene travertine (all of the Mammoth Hot Springs (YNP) and (Cakmak Quarry, Turkey). Genomic DNA entombed during rapid (up to 5 mm/day) travertine deposition and preserved within CaCO3 fluid inclusions and between crystals, was extracted via bulk rock drilling under sterile clean room conditions. Pooled 16S rRNA gene sequence libraries were constructed via polymerase chain reactions (PCRs), terminal-restriction fragment length polymorphisms (T-RFLP), and MiSeq amplicon sequencing. Blast searches using multiple web-based bioinformatics tools identified over 400 operational taxonomic units (OTUs) affiliated with a total of 19 phyla (16 phyla and 3 candidate phyla) within the Domain Bacteria. Previous analyses of the living microbial communities inhabiting modern active PSF depositional environments at MHS have provided a baseline with which to compare the ancient travertine analyses. Combined results from both MHS and Deniz…
author: Asangba, Abigail E.
language: en
keywords: Travertine, Hot springs, Ancient DNA, Phylogeny, Physiotypes
date published: 2015-07-22T22:17:44Z
date modified: 2026-07-22T22:26:11Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/78498