{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/78443"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/78443","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Genetic introgression from Glycine tomentella to soybean to increase seed yield","abstract":"The student, Abraham Akpertey, accepted the attached license on 2015-04-21 at 13:53.","abstract_html":"The student, Abraham Akpertey, accepted the attached license on 2015-04-21 at 13:53.","abstract_has_math":false,"creators":["Akpertey, Abraham"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Crop Sciences","degree_department":null,"school":null,"contributors":["Nelson, Randall L.","Diers, Brian W.","Hudson, Matthew E.","Brown, Patrick J."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-07-22T22:17:17Z","date_published":"2015-07-22T22:17:17Z","updated_at":"2026-07-22T22:26:11Z","subjects":["Glycine tomentella","Wide hybridization","Perennial species","tertiary gene pool","Soybean germplasm","Introgression","Single nucleotide polymorphism","Genotyping by sequencing"],"languages":["en"],"rights":["Copyright 2015 Abraham Akpertey"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/78443","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Nelson, Randall L.","Diers, Brian W.","Hudson, Matthew E.","Brown, Patrick J."]},{"key":"dc:creator","label":"Author","values":["Akpertey, Abraham"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-07-22T22:17:17Z","2015-05","2015-04-22","2015-5"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Glycine tomentella","Wide hybridization","Perennial species","tertiary gene pool","Soybean germplasm","Introgression","Single nucleotide polymorphism","Genotyping by sequencing"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2015 Abraham Akpertey"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/78443"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The student, Abraham Akpertey, accepted the attached license on 2015-04-21 at 13:53.","The student, Abraham Akpertey, submitted this Dissertation for approval on 2015-04-21 at 14:23.","This Dissertation was approved for publication on 2015-04-22 at 16:27.","DSpace SAF Submission Ingestion Package generated from Vireo submission #7991 on 2015-07-22 at 10:33:07","Made available in DSpace on 2015-07-22T22:17:17Z (GMT). No. of bitstreams: 2 AKPERTEY-DISSERTATION-2015.pdf: 1457740 bytes, checksum: c4333d582b3d162015de270ffaeb947d (MD5) LICENSE.txt: 4213 bytes, checksum: eae5d0d51a9e14a4866d8ce73b9febf3 (MD5) Previous issue date: 2015-04-22","Commercial soybean [Glycine max (L.) Merr.] breeding in the U.S. currently relies on a narrow genetic base in which more than half of the genetic contribution, calculated by pedigree analysis, comes from only 5 ancestral lines. For decades, but more intensely in recent years, efforts have been made to incorporate exotic soybean germplasm into the breeding pool. G. tomentella (2n = 78), native to Australia, is a perennial relative in the tertiary gene pool of soybean. Although G. tomentella has been shown to have high levels of resistance to several diseases, including soybean rust and soybean cyst nematode, no effort has been devoted to utilizing this species to increase seed yield. The objectives of this research are to identify high yielding lines derived from backcrosses between the cultivar Dwight and G. tomentella PI 441001 (2n = 78), and to determine the genetic contribution of PI 441001 to these lines. PI 441001 was crossed to Dwight and immature seed rescue was used to produce a sterile F1 plant. Amphidiploid plants (2n = 118) were produced by treating the F1 hybrid with colchicine. Amphidiploid plants were backcrossed to Dwight to obtain BC1 plants. A series of backcrosses were made with seven different BC2 plants to obtain BC3, BC4, BC5, and BC6 lines with 2n = 40 chromosomes. Preliminary yield testing of inbred lines from these crosses was used to select 180 lines in maturity groups (MG) II, III, and IV. Yield and other agronomic data were collected in two replication tests at six to eight locations in Ohio, Illinois, Missouri and Nebraska in 2013 and 2014. These data identified experimental lines in all three MGs that are significantly higher yielding than the recurrent parent. All experimental and parental lines were genotyped using genotyping by sequencing. G. tomentella introgressions were tested for associations with time of flowering and maturity, height, lodging, and yield. Significant associations between G. tomentella single nucleotide polymorphism introgressions and all phenotypic traits were identified. On average, 3.9, 2.5, 2.5, and 3% G. tomentella alleles were introgressed into BC3, BC4, BC5, and BC6 derived lines, respectively.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2015-07-22 without embargo terms"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Genetic introgression from Glycine tomentella to soybean to increase seed yield"]}]}],"canonical_facts":{"dc:contributor":["Nelson, Randall L.","Diers, Brian W.","Hudson, Matthew E.","Brown, Patrick J."],"dc:creator":["Akpertey, Abraham"],"dc:date":["2015-07-22T22:17:17Z","2015-05","2015-04-22","2015-5"],"dc:description":["The student, Abraham Akpertey, accepted the attached license on 2015-04-21 at 13:53.","The student, Abraham Akpertey, submitted this Dissertation for approval on 2015-04-21 at 14:23.","This Dissertation was approved for publication on 2015-04-22 at 16:27.","DSpace SAF Submission Ingestion Package generated from Vireo submission #7991 on 2015-07-22 at 10:33:07","Made available in DSpace on 2015-07-22T22:17:17Z (GMT). No. of bitstreams: 2 AKPERTEY-DISSERTATION-2015.pdf: 1457740 bytes, checksum: c4333d582b3d162015de270ffaeb947d (MD5) LICENSE.txt: 4213 bytes, checksum: eae5d0d51a9e14a4866d8ce73b9febf3 (MD5) Previous issue date: 2015-04-22","Commercial soybean [Glycine max (L.) Merr.] breeding in the U.S. currently relies on a narrow genetic base in which more than half of the genetic contribution, calculated by pedigree analysis, comes from only 5 ancestral lines. For decades, but more intensely in recent years, efforts have been made to incorporate exotic soybean germplasm into the breeding pool. G. tomentella (2n = 78), native to Australia, is a perennial relative in the tertiary gene pool of soybean. Although G. tomentella has been shown to have high levels of resistance to several diseases, including soybean rust and soybean cyst nematode, no effort has been devoted to utilizing this species to increase seed yield. The objectives of this research are to identify high yielding lines derived from backcrosses between the cultivar Dwight and G. tomentella PI 441001 (2n = 78), and to determine the genetic contribution of PI 441001 to these lines. PI 441001 was crossed to Dwight and immature seed rescue was used to produce a sterile F1 plant. Amphidiploid plants (2n = 118) were produced by treating the F1 hybrid with colchicine. Amphidiploid plants were backcrossed to Dwight to obtain BC1 plants. A series of backcrosses were made with seven different BC2 plants to obtain BC3, BC4, BC5, and BC6 lines with 2n = 40 chromosomes. Preliminary yield testing of inbred lines from these crosses was used to select 180 lines in maturity groups (MG) II, III, and IV. Yield and other agronomic data were collected in two replication tests at six to eight locations in Ohio, Illinois, Missouri and Nebraska in 2013 and 2014. These data identified experimental lines in all three MGs that are significantly higher yielding than the recurrent parent. All experimental and parental lines were genotyped using genotyping by sequencing. G. tomentella introgressions were tested for associations with time of flowering and maturity, height, lodging, and yield. Significant associations between G. tomentella single nucleotide polymorphism introgressions and all phenotypic traits were identified. On average, 3.9, 2.5, 2.5, and 3% G. tomentella alleles were introgressed into BC3, BC4, BC5, and BC6 derived lines, respectively.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2015-07-22 without embargo terms"],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/78443"],"dc:language":["en"],"dc:rights":["Copyright 2015 Abraham Akpertey"],"dc:subject":["Glycine tomentella","Wide hybridization","Perennial species","tertiary gene pool","Soybean germplasm","Introgression","Single nucleotide polymorphism","Genotyping by sequencing"],"dc:title":["Genetic introgression from Glycine tomentella to soybean to increase seed yield"],"dc:type":["text"],"thesis:degree_discipline":["Crop Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:11Z"}