{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/77666"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/77666","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Compartmental Analysis of Roots in Intact Rapidly-Growing Spergularia Marina and Lactuca Sativa: Partial Characterization of the Symplasms Functional in the Radial Transport of Sodium Ion and Potassium Ion","abstract":"Techniques of compartmental analysis were adapted to the study of intact roots of rapidly-growing Spergularia marine and Lactuca sativa. Using large numbers of plants short time-courses of uptake and chase, $\\sp{42}$K$\\sp+$ and $\\sp{22}$Na$\\sp+$ transport could be resolved, even during a chase following a brief 10 minute labeling period. The use of intact plant systems allowed distinction of that portion of the isotope flux into the root, associated with the ion-conducting symplasms.","abstract_html":"Techniques of compartmental analysis were adapted to the study of intact roots of rapidly-growing Spergularia marine and Lactuca sativa. Using large numbers of plants short time-courses of uptake and chase, $\\sp{42}$K$\\sp+$ and $\\sp{22}$Na$\\sp+$ transport could be resolved, even during a chase following a brief 10 minute labeling period. The use of intact plant systems allowed distinction of that portion of the isotope flux into the root, associated with the ion-conducting symplasms.","abstract_has_math":true,"creators":["Lazof, Dennis Boyd"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Botany","degree_department":null,"school":null,"contributors":["Cheeseman, John M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1987,"date_issued":"1987","date_published":"1987","updated_at":"2026-07-22T22:26:11Z","subjects":["Biology, Plant Physiology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8803105"],"render_values":[{"text":"(UMI)AAI8803105","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/77666","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Cheeseman, John M."]},{"key":"dc:creator","label":"Author","values":["Lazof, Dennis Boyd"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["1987","2015-05-14T15:19:18Z","10000-01-01"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Botany"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Plant Physiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/77666","(UMI)AAI8803105"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Techniques of compartmental analysis were adapted to the study of intact roots of rapidly-growing Spergularia marine and Lactuca sativa. Using large numbers of plants short time-courses of uptake and chase, $\\sp{42}$K$\\sp+$ and $\\sp{22}$Na$\\sp+$ transport could be resolved, even during a chase following a brief 10 minute labeling period. The use of intact plant systems allowed distinction of that portion of the isotope flux into the root, associated with the ion-conducting symplasms.","A small compartment, which rapidly (t$\\sb{.5}$ $<$ 1 min) exchanges with the external medium was implicated in the radial transport of N$\\sp+$, accounting for the observed obtention of linear translocation rates within minutes of transferring to labeled solution. At the 90 mol m$\\sp{-3}$ Na$\\sp+$ steady-state, 90% of the contents of this compartment exchanged out to the medium during a chase, while the remaining 10% translocated to the shoot in the first few minutes of the chase. The ion contents of this compartment varied in proportion to the external ion concentration.","When K$\\sp+$ was at a high external concentration, labeled K$\\sp+$ exchanged into this same symplasm, but chasing a short pulse indicated that K$\\sp+$ transport to the xylem was not through a rapidly-exchanging compartment. At physiological concentrations of K$\\sp+$ the evidence indicated that transport of K$\\sp+$ across the root proceeded through a compartment which was not exchanging rapidly with the external medium. The rise to a linear rate of isotope translocation was gradual and translocation during a chase, following a brief pulse, was prolonged, indicating that this compartment retained its specific activity for a considerable period.","In computer simulation modeling, it was shown that specialized ion-transporting symplasms needed to be incorporated into the compartmental model. An alternative model with specialized conducting compartments for Na$\\sp+$ and K$\\sp+$ was required to overcome the major difficulties. In the case of K$\\sp+$, it was necessary to virtually eliminate uptake from the external medium by exchange in order to simulate the experimental results.","Made available in DSpace on 2015-05-14T15:19:18Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 8803105.PDF: 4433401 bytes, checksum: 560ab3d387fa02dac79bea1cbb4744e6 (MD5) Previous issue date: 1987","Embargo set by: Seth Robbins for item 78876 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","160 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1987."]},{"key":"dc:title","label":"Title","values":["Compartmental Analysis of Roots in Intact Rapidly-Growing Spergularia Marina and Lactuca Sativa: Partial Characterization of the Symplasms Functional in the Radial Transport of Sodium Ion and Potassium Ion"]}]}],"canonical_facts":{"dc:contributor":["Cheeseman, John M."],"dc:creator":["Lazof, Dennis Boyd"],"dc:date":["1987","2015-05-14T15:19:18Z","10000-01-01"],"dc:description":["Techniques of compartmental analysis were adapted to the study of intact roots of rapidly-growing Spergularia marine and Lactuca sativa. Using large numbers of plants short time-courses of uptake and chase, $\\sp{42}$K$\\sp+$ and $\\sp{22}$Na$\\sp+$ transport could be resolved, even during a chase following a brief 10 minute labeling period. The use of intact plant systems allowed distinction of that portion of the isotope flux into the root, associated with the ion-conducting symplasms.","A small compartment, which rapidly (t$\\sb{.5}$ $<$ 1 min) exchanges with the external medium was implicated in the radial transport of N$\\sp+$, accounting for the observed obtention of linear translocation rates within minutes of transferring to labeled solution. At the 90 mol m$\\sp{-3}$ Na$\\sp+$ steady-state, 90% of the contents of this compartment exchanged out to the medium during a chase, while the remaining 10% translocated to the shoot in the first few minutes of the chase. The ion contents of this compartment varied in proportion to the external ion concentration.","When K$\\sp+$ was at a high external concentration, labeled K$\\sp+$ exchanged into this same symplasm, but chasing a short pulse indicated that K$\\sp+$ transport to the xylem was not through a rapidly-exchanging compartment. At physiological concentrations of K$\\sp+$ the evidence indicated that transport of K$\\sp+$ across the root proceeded through a compartment which was not exchanging rapidly with the external medium. The rise to a linear rate of isotope translocation was gradual and translocation during a chase, following a brief pulse, was prolonged, indicating that this compartment retained its specific activity for a considerable period.","In computer simulation modeling, it was shown that specialized ion-transporting symplasms needed to be incorporated into the compartmental model. An alternative model with specialized conducting compartments for Na$\\sp+$ and K$\\sp+$ was required to overcome the major difficulties. In the case of K$\\sp+$, it was necessary to virtually eliminate uptake from the external medium by exchange in order to simulate the experimental results.","Made available in DSpace on 2015-05-14T15:19:18Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 8803105.PDF: 4433401 bytes, checksum: 560ab3d387fa02dac79bea1cbb4744e6 (MD5) Previous issue date: 1987","Embargo set by: Seth Robbins for item 78876 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","160 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1987."],"dc:identifier":["http://hdl.handle.net/2142/77666","(UMI)AAI8803105"],"dc:language":["eng"],"dc:subject":["Biology, Plant Physiology"],"dc:title":["Compartmental Analysis of Roots in Intact Rapidly-Growing Spergularia Marina and Lactuca Sativa: Partial Characterization of the Symplasms Functional in the Radial Transport of Sodium Ion and Potassium Ion"],"dc:type":["text"],"thesis:degree_discipline":["Botany"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:11Z"}