{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/77658"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/77658","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Establishment, Optimization, and Characterization of Photoautotrophic Soybean Suspension Cultures","abstract":"A novel system was designed which allowed the photoautotrophic growth of a previously established chlorophyllous soybean cell line. The parameters of growth (fresh weight, dry weight, chlorophyll content, medium pH, dark O(,2) uptake, dark CO(,2) fixation, and light-driven CO(,2) fixation) were ascertained throughout the entire subculture period (14 days). Of particular interest was the almost nonexistent lag phase in the growth cycle and the rapid drops in medium pH and dark CO(,2) uptake (respiration). Light-driven CO(,2) fixation rates easily accounted for the increase in dry weight exhibited by the culture. Photoautotrophic growth was completely inhibited by 0.5 micromolar DCMU, a photosynthetic herbicide, while cells grown in the dark on 3% sucrose were inhibited less than 10% by the DCMU. The cells would not grow in the dark under photoautotrophic conditions (5% CO(,2), no sucrose). A comprehensive examination was made of all medium components in an effort to determine if any were limiting or supraoptimal for cell growth. Indole-3-acetic acid was found to be a superior auxin with five to ten milligrams per liter being the most effective. No other single component was found to significantly increase growth when varied in concentration. Photoautotrophic soybean cells were shown to have typical nitrate reductase activity peaks six days after subculture but did not evolve significant amounts of nitrogenous gas, a trait associated with the constitutive nitrate reductase activity found in young soybean leaves and cotyledons. The cells accumulated as well as excreted large quantities of asparagine, serine, and glutamine suggesting that nitrogen is stored in this manner. Under high CO(,2) levels, photoautotrophic soybean cells fixed most of the CO(,2) into the organic acids malate and citrate with most of the remaining fixed CO(,2) found in aspartate and glutamate. Under low CO(,2) levels, CO(,2) was fixed initially into 3-phosphoglyceric acid and hexose monophosphates. These quickly declined as a percentage while serine, glycine, aspartate and other amino acids gradually became the dominant fixation products.","abstract_html":"A novel system was designed which allowed the photoautotrophic growth of a previously established chlorophyllous soybean cell line. The parameters of growth (fresh weight, dry weight, chlorophyll content, medium pH, dark O(,2) uptake, dark CO(,2) fixation, and light-driven CO(,2) fixation) were ascertained throughout the entire subculture period (14 days). Of particular interest was the almost nonexistent lag phase in the growth cycle and the rapid drops in medium pH and dark CO(,2) uptake (respiration). Light-driven CO(,2) fixation rates easily accounted for the increase in dry weight exhibited by the culture. Photoautotrophic growth was completely inhibited by 0.5 micromolar DCMU, a photosynthetic herbicide, while cells grown in the dark on 3% sucrose were inhibited less than 10% by the DCMU. The cells would not grow in the dark under photoautotrophic conditions (5% CO(,2), no sucrose). A comprehensive examination was made of all medium components in an effort to determine if any were limiting or supraoptimal for cell growth. Indole-3-acetic acid was found to be a superior auxin with five to ten milligrams per liter being the most effective. No other single component was found to significantly increase growth when varied in concentration. Photoautotrophic soybean cells were shown to have typical nitrate reductase activity peaks six days after subculture but did not evolve significant amounts of nitrogenous gas, a trait associated with the constitutive nitrate reductase activity found in young soybean leaves and cotyledons. The cells accumulated as well as excreted large quantities of asparagine, serine, and glutamine suggesting that nitrogen is stored in this manner. Under high CO(,2) levels, photoautotrophic soybean cells fixed most of the CO(,2) into the organic acids malate and citrate with most of the remaining fixed CO(,2) found in aspartate and glutamate. Under low CO(,2) levels, CO(,2) was fixed initially into 3-phosphoglyceric acid and hexose monophosphates. These quickly declined as a percentage while serine, glycine, aspartate and other amino acids gradually became the dominant fixation products.","abstract_has_math":false,"creators":["Horn, Michael Eugene"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Botany","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-05-14T15:19:15Z","date_published":"2015-05-14T15:19:15Z","updated_at":"2026-07-22T22:26:11Z","subjects":["Agriculture, Plant Culture"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8502179"],"render_values":[{"text":"(UMI)AAI8502179","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/77658","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Horn, Michael Eugene"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-05-14T15:19:15Z","10000-01-01","1984"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Botany"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Plant Culture"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/77658","(UMI)AAI8502179"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["A novel system was designed which allowed the photoautotrophic growth of a previously established chlorophyllous soybean cell line. The parameters of growth (fresh weight, dry weight, chlorophyll content, medium pH, dark O(,2) uptake, dark CO(,2) fixation, and light-driven CO(,2) fixation) were ascertained throughout the entire subculture period (14 days). Of particular interest was the almost nonexistent lag phase in the growth cycle and the rapid drops in medium pH and dark CO(,2) uptake (respiration). Light-driven CO(,2) fixation rates easily accounted for the increase in dry weight exhibited by the culture. Photoautotrophic growth was completely inhibited by 0.5 micromolar DCMU, a photosynthetic herbicide, while cells grown in the dark on 3% sucrose were inhibited less than 10% by the DCMU. The cells would not grow in the dark under photoautotrophic conditions (5% CO(,2), no sucrose). A comprehensive examination was made of all medium components in an effort to determine if any were limiting or supraoptimal for cell growth. Indole-3-acetic acid was found to be a superior auxin with five to ten milligrams per liter being the most effective. No other single component was found to significantly increase growth when varied in concentration. Photoautotrophic soybean cells were shown to have typical nitrate reductase activity peaks six days after subculture but did not evolve significant amounts of nitrogenous gas, a trait associated with the constitutive nitrate reductase activity found in young soybean leaves and cotyledons. The cells accumulated as well as excreted large quantities of asparagine, serine, and glutamine suggesting that nitrogen is stored in this manner. Under high CO(,2) levels, photoautotrophic soybean cells fixed most of the CO(,2) into the organic acids malate and citrate with most of the remaining fixed CO(,2) found in aspartate and glutamate. Under low CO(,2) levels, CO(,2) was fixed initially into 3-phosphoglyceric acid and hexose monophosphates. These quickly declined as a percentage while serine, glycine, aspartate and other amino acids gradually became the dominant fixation products.","Made available in DSpace on 2015-05-14T15:19:15Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 8502179.PDF: 4448503 bytes, checksum: 16c1a6ed06f03640e7b810a31dbbcc5e (MD5) Previous issue date: 1984","Embargo set by: Seth Robbins for item 78868 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","130 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1984."]},{"key":"dc:title","label":"Title","values":["Establishment, Optimization, and Characterization of Photoautotrophic Soybean Suspension Cultures"]}]}],"canonical_facts":{"dc:creator":["Horn, Michael Eugene"],"dc:date":["2015-05-14T15:19:15Z","10000-01-01","1984"],"dc:description":["A novel system was designed which allowed the photoautotrophic growth of a previously established chlorophyllous soybean cell line. The parameters of growth (fresh weight, dry weight, chlorophyll content, medium pH, dark O(,2) uptake, dark CO(,2) fixation, and light-driven CO(,2) fixation) were ascertained throughout the entire subculture period (14 days). Of particular interest was the almost nonexistent lag phase in the growth cycle and the rapid drops in medium pH and dark CO(,2) uptake (respiration). Light-driven CO(,2) fixation rates easily accounted for the increase in dry weight exhibited by the culture. Photoautotrophic growth was completely inhibited by 0.5 micromolar DCMU, a photosynthetic herbicide, while cells grown in the dark on 3% sucrose were inhibited less than 10% by the DCMU. The cells would not grow in the dark under photoautotrophic conditions (5% CO(,2), no sucrose). A comprehensive examination was made of all medium components in an effort to determine if any were limiting or supraoptimal for cell growth. Indole-3-acetic acid was found to be a superior auxin with five to ten milligrams per liter being the most effective. No other single component was found to significantly increase growth when varied in concentration. Photoautotrophic soybean cells were shown to have typical nitrate reductase activity peaks six days after subculture but did not evolve significant amounts of nitrogenous gas, a trait associated with the constitutive nitrate reductase activity found in young soybean leaves and cotyledons. The cells accumulated as well as excreted large quantities of asparagine, serine, and glutamine suggesting that nitrogen is stored in this manner. Under high CO(,2) levels, photoautotrophic soybean cells fixed most of the CO(,2) into the organic acids malate and citrate with most of the remaining fixed CO(,2) found in aspartate and glutamate. Under low CO(,2) levels, CO(,2) was fixed initially into 3-phosphoglyceric acid and hexose monophosphates. These quickly declined as a percentage while serine, glycine, aspartate and other amino acids gradually became the dominant fixation products.","Made available in DSpace on 2015-05-14T15:19:15Z (GMT). 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