{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/77643"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/77643","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Kappa Opioid Binding Characteristics in Rat Central Nervous System Homogenates (Binding)","abstract":"Multiple types of opiate receptors have been proposed on the basis of pharmacological and biochemical studies. Mu and delta opiate receptors have been the most widely studied because of the availability of tissues enriched in these specific receptor subtypes and the availability of specific radioligands.","abstract_html":"Multiple types of opiate receptors have been proposed on the basis of pharmacological and biochemical studies. Mu and delta opiate receptors have been the most widely studied because of the availability of tissues enriched in these specific receptor subtypes and the availability of specific radioligands.","abstract_has_math":false,"creators":["Mack, Kenneth Joseph"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-05-14T15:18:36Z","date_published":"2015-05-14T15:18:36Z","updated_at":"2026-07-22T22:26:11Z","subjects":["Biology, General"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8521824"],"render_values":[{"text":"(UMI)AAI8521824","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/77643","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Mack, Kenneth Joseph"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-05-14T15:18:36Z","10000-01-01","1985"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, General"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/77643","(UMI)AAI8521824"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Multiple types of opiate receptors have been proposed on the basis of pharmacological and biochemical studies. Mu and delta opiate receptors have been the most widely studied because of the availability of tissues enriched in these specific receptor subtypes and the availability of specific radioligands.","Data presented in this thesis has compared brain and spinal cord kappa opioid binding in an attempt to identify an enriched population of kappa sites for study. The relative percentage of kappa binding was similar in both tissues, although brain contained a higher absolute number of sites. No significant differences in opiate affinity characteristics were identified between the two tissues.","Additionally, paradigms using ('3)H-ethylketocyclazocine and ('3)H-diprenorphine were studied under conditions where mu and delta sites were blocked. Under these blocked conditions, ('3)H-ethylketocyclazoince labelled a single site as defined by kinetic and equilibrium analysis which showed high affinity for prototypic kappa ligands. Blocked ('3)H-diprenorphine binding data suggested the labelling of an additional binding site.","Finally, the effects of GTP and sodium were investigated on putative kappa opioid binding using the blocked ('3)H-ethylketocyclazocine paradigm. These data suggested that kappa sites are GTP and sodium sensitive, however, ('3)H-ethylketocyclazocine binding to kappa sites is less sensitive to GTP than its binding to other sites.","In summary, this thesis has been an effort to understand the binding characteristics of the kappa opioid binding site/receptor. Investigations were performed to compare rationally selected CNS tissue types and available radioligands in order to find an appropriate paradigm that could characterize the kappa opioid binding site. This paradigm was then used to gather information concerning the potential role of the kappa receptor in the modulation of adenylate cyclase.","Made available in DSpace on 2015-05-14T15:18:36Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 8521824.PDF: 3250136 bytes, checksum: 9ae90d40bc0e9347704a12a3a985af9f (MD5) Previous issue date: 1985","Embargo set by: Seth Robbins for item 78853 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","100 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1985."]},{"key":"dc:title","label":"Title","values":["Kappa Opioid Binding Characteristics in Rat Central Nervous System Homogenates (Binding)"]}]}],"canonical_facts":{"dc:creator":["Mack, Kenneth Joseph"],"dc:date":["2015-05-14T15:18:36Z","10000-01-01","1985"],"dc:description":["Multiple types of opiate receptors have been proposed on the basis of pharmacological and biochemical studies. Mu and delta opiate receptors have been the most widely studied because of the availability of tissues enriched in these specific receptor subtypes and the availability of specific radioligands.","Data presented in this thesis has compared brain and spinal cord kappa opioid binding in an attempt to identify an enriched population of kappa sites for study. The relative percentage of kappa binding was similar in both tissues, although brain contained a higher absolute number of sites. No significant differences in opiate affinity characteristics were identified between the two tissues.","Additionally, paradigms using ('3)H-ethylketocyclazocine and ('3)H-diprenorphine were studied under conditions where mu and delta sites were blocked. Under these blocked conditions, ('3)H-ethylketocyclazoince labelled a single site as defined by kinetic and equilibrium analysis which showed high affinity for prototypic kappa ligands. Blocked ('3)H-diprenorphine binding data suggested the labelling of an additional binding site.","Finally, the effects of GTP and sodium were investigated on putative kappa opioid binding using the blocked ('3)H-ethylketocyclazocine paradigm. These data suggested that kappa sites are GTP and sodium sensitive, however, ('3)H-ethylketocyclazocine binding to kappa sites is less sensitive to GTP than its binding to other sites.","In summary, this thesis has been an effort to understand the binding characteristics of the kappa opioid binding site/receptor. Investigations were performed to compare rationally selected CNS tissue types and available radioligands in order to find an appropriate paradigm that could characterize the kappa opioid binding site. This paradigm was then used to gather information concerning the potential role of the kappa receptor in the modulation of adenylate cyclase.","Made available in DSpace on 2015-05-14T15:18:36Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 8521824.PDF: 3250136 bytes, checksum: 9ae90d40bc0e9347704a12a3a985af9f (MD5) Previous issue date: 1985","Embargo set by: Seth Robbins for item 78853 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","100 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1985."],"dc:identifier":["http://hdl.handle.net/2142/77643","(UMI)AAI8521824"],"dc:language":["eng"],"dc:subject":["Biology, General"],"dc:title":["Kappa Opioid Binding Characteristics in Rat Central Nervous System Homogenates (Binding)"],"dc:type":["text"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:11Z"}