Purification of Vitamin D Binding Protein and Its Role in the Uptake of 25-Hydroxyvitamin D by Cultured Kidney Cells (Mdck Cells, Tissue Culture, Dbp)
type: CreativeWork
description: This thesis reports the isolation of vitamin D binding protein (DBP) and its use in studies investigating the uptake of 25(OH)D(,3) by kidney cell lines. DBP was isolated from pig plasma in three steps: chromatography on DEAE cellulose and DEAE Sephadex, followed by chromatofocusing. The procedure represents a shorter purification scheme than those previously reported. Antibodies to DBP were produced in rabbits and coupled to cyanogen bromide-activated Sepharose 4B. With this antibody affinity column, DBP was isolated fron pig plasma to a purity similar to that in the initial purification in a one-step procedure. Holo- and apo-DBP did not co-chromatograph upon chromatofocusing, which was used to separate the two forms. Holo-DBP was used as the source of 25(OH)D(,3) to study its uptake by kidney cells.
abstract: This thesis reports the isolation of vitamin D binding protein (DBP) and its use in studies investigating the uptake of 25(OH)D(,3) by kidney cell lines. DBP was isolated from pig plasma in three steps: chromatography on DEAE cellulose and DEAE Sephadex, followed by chromatofocusing. The procedure represents a shorter purification scheme than those previously reported. Antibodies to DBP were produced in rabbits and coupled to cyanogen bromide-activated Sepharose 4B. With this antibody affinity column, DBP was isolated fron pig plasma to a purity similar to that in the initial purification in a one-step procedure. Holo- and apo-DBP did not co-chromatograph upon chromatofocusing, which was used to separate the two forms. Holo-DBP was used as the source of 25(OH)D(,3) to study its uptake by kidney cells.
author: Keenan, Michael James
language: eng
keywords: Biology, General
date published: 2015-05-13T15:44:19Z
date modified: 2026-07-22T22:26:10Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/77450