{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/77445"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/77445","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The Influence of Dietary Fat on The Cerebral Microvasculature: Compositional, Prostaglandin Synthetic, and Permeability Studies","abstract":"In order to investigate the effects of long-term feeding of single fat sources, differing in essential fatty acids, on membrane-based phenomena in the cerebral cortex and microvasculature the following studies were conducted. Rats fed purified diets containing 10% corn, hydrogenated coconut, or linseed oils through two generations were sacrificed and the following items measured: fatty acyl composition of phospholipids, prostaglandin F(,2(alpha)) and prostacyclin synthesis by cortical slices and microvessels, respectively, kinetic parameters of adenosine uptake, and the potential involvement of eicosanoids in the uptake process.","abstract_html":"In order to investigate the effects of long-term feeding of single fat sources, differing in essential fatty acids, on membrane-based phenomena in the cerebral cortex and microvasculature the following studies were conducted. Rats fed purified diets containing 10% corn, hydrogenated coconut, or linseed oils through two generations were sacrificed and the following items measured: fatty acyl composition of phospholipids, prostaglandin F(,2(alpha)) and prostacyclin synthesis by cortical slices and microvessels, respectively, kinetic parameters of adenosine uptake, and the potential involvement of eicosanoids in the uptake process.","abstract_has_math":false,"creators":["Brown, Michael Lee"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Food Science","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-05-13T15:44:18Z","date_published":"2015-05-13T15:44:18Z","updated_at":"2026-07-22T22:26:10Z","subjects":["Health Sciences, Nutrition"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8409872"],"render_values":[{"text":"(UMI)AAI8409872","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/77445","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Brown, Michael Lee"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-05-13T15:44:18Z","10000-01-01","1983"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Health Sciences, Nutrition"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/77445","(UMI)AAI8409872"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["In order to investigate the effects of long-term feeding of single fat sources, differing in essential fatty acids, on membrane-based phenomena in the cerebral cortex and microvasculature the following studies were conducted. Rats fed purified diets containing 10% corn, hydrogenated coconut, or linseed oils through two generations were sacrificed and the following items measured: fatty acyl composition of phospholipids, prostaglandin F(,2(alpha)) and prostacyclin synthesis by cortical slices and microvessels, respectively, kinetic parameters of adenosine uptake, and the potential involvement of eicosanoids in the uptake process.","Major changes were found in the fatty acyl compositions of the phospholipids investigated in both tissues. Notable were changes in the 20- and 22-carbon polyenes and most dramatically in the phosphatidyl ethanolamine fractions. The changes were accompanied by a decrease in prostaglandin synthesis in both tissues by either linseed or hydrogenated coconut oil feeding. This was explained by the presence of certain fatty acids acting as competitive inhibitors as well as diminished arachidonate levels. In measuring kinetic parameters, the V(,max) of adenosine uptake in cerebral microvessels was greatest when hydrogenated coconut oil was fed, intermediate in the linseed oil group, and lowest with corn oil. Km measurements were not substantially different. This was explained as a function of membrane saturation, alterations in arachidonate presence and potential eicosanoid involvement. Involvement of eicosanoids was checked by incubations of microvessels with various inhibitors or activators. Results were inconclusive.","The prostaglandin synthetic studies demonstrate that prostacyclin synthesis is subject to dietary manipulation and raise questions concerning the usefulness of feeding diets high in (omega)-3 fatty acids as a therapeutic agent in controlling hypertension, thrombus formation, isochemia, etc., since diminished prostacyclin is associated with increased vascular tone. The kinetic studies are of interest because they show that long-term dietary fat patterns can influence blood-brain barrier transport. Such changes have been linked to many neuropathologies and these findings point to a predisposition to such pathologies by specific populations.","Made available in DSpace on 2015-05-13T15:44:18Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 8409872.PDF: 2733135 bytes, checksum: 526d73fbbe554031cdbeb305cb92fbca (MD5) Previous issue date: 1983","Embargo set by: Seth Robbins for item 78656 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","80 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1983."]},{"key":"dc:title","label":"Title","values":["The Influence of Dietary Fat on The Cerebral Microvasculature: Compositional, Prostaglandin Synthetic, and Permeability Studies"]}]}],"canonical_facts":{"dc:creator":["Brown, Michael Lee"],"dc:date":["2015-05-13T15:44:18Z","10000-01-01","1983"],"dc:description":["In order to investigate the effects of long-term feeding of single fat sources, differing in essential fatty acids, on membrane-based phenomena in the cerebral cortex and microvasculature the following studies were conducted. Rats fed purified diets containing 10% corn, hydrogenated coconut, or linseed oils through two generations were sacrificed and the following items measured: fatty acyl composition of phospholipids, prostaglandin F(,2(alpha)) and prostacyclin synthesis by cortical slices and microvessels, respectively, kinetic parameters of adenosine uptake, and the potential involvement of eicosanoids in the uptake process.","Major changes were found in the fatty acyl compositions of the phospholipids investigated in both tissues. Notable were changes in the 20- and 22-carbon polyenes and most dramatically in the phosphatidyl ethanolamine fractions. The changes were accompanied by a decrease in prostaglandin synthesis in both tissues by either linseed or hydrogenated coconut oil feeding. This was explained by the presence of certain fatty acids acting as competitive inhibitors as well as diminished arachidonate levels. In measuring kinetic parameters, the V(,max) of adenosine uptake in cerebral microvessels was greatest when hydrogenated coconut oil was fed, intermediate in the linseed oil group, and lowest with corn oil. Km measurements were not substantially different. This was explained as a function of membrane saturation, alterations in arachidonate presence and potential eicosanoid involvement. Involvement of eicosanoids was checked by incubations of microvessels with various inhibitors or activators. Results were inconclusive.","The prostaglandin synthetic studies demonstrate that prostacyclin synthesis is subject to dietary manipulation and raise questions concerning the usefulness of feeding diets high in (omega)-3 fatty acids as a therapeutic agent in controlling hypertension, thrombus formation, isochemia, etc., since diminished prostacyclin is associated with increased vascular tone. The kinetic studies are of interest because they show that long-term dietary fat patterns can influence blood-brain barrier transport. Such changes have been linked to many neuropathologies and these findings point to a predisposition to such pathologies by specific populations.","Made available in DSpace on 2015-05-13T15:44:18Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 8409872.PDF: 2733135 bytes, checksum: 526d73fbbe554031cdbeb305cb92fbca (MD5) Previous issue date: 1983","Embargo set by: Seth Robbins for item 78656 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","80 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1983."],"dc:identifier":["http://hdl.handle.net/2142/77445","(UMI)AAI8409872"],"dc:language":["eng"],"dc:subject":["Health Sciences, Nutrition"],"dc:title":["The Influence of Dietary Fat on The Cerebral Microvasculature: Compositional, Prostaglandin Synthetic, and Permeability Studies"],"dc:type":["text"],"thesis:degree_discipline":["Food Science"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:10Z"}