{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/77444"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/77444","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Effect of Fatty Acid Modification on Prostacyclin Production in Cultured Human Endothelial Cells (Arachidonate, Elcosapentaenoate)","abstract":"An endothelial cell culture system was used to compare the effects of endogenous arachidonic acid (AA) and eicosapentaenoic acid (EPA) on prostacyclin synthesis. Supplemental levels of fatty acids (20-150 (mu)M) were supplied as sodium salts bound to bovine serum albumin (3:1 mole ratio) and added to M-199 with 20% fetal bovine serum. Both AA and EPA were readily incorporated into neutral and phospholipid fractions to serve as endogenous precursors for prostacyclin (PGI(,2)) synthesis. Thrombin (.5 U/ml) was used to stimulate prostacyclin synthesis. AA enrichment did not result in an increased PGI(,2) synthesis while EPA enrichment decreased PGI(,2) synthesis as measured by radioimmunoassay to 6-keto-PGF(,1(alpha)). Radiolabelled fatty acid eicosanoid products were separated by reverse phase HPLC. Unconverted fatty acid was the major product released upon thrombin stimulation in both cases. ('3)H-AA supplemented cells also released PGI(,2) and HETE in lesser amounts while ('14)C-EPA supplemented cells released only minimal amounts of PGI(,3) and HEPE. The relative percent incorporation of radiolabelled ('3)H-AA and ('14)C-EPA into cellular lipids was determined after two-dimensional thin-layer chromatography. Neutral lipid contained the vast majority of radiolabel ((TURN)50%). Of the phospholipids, phosphatidylcholine contained the greatest percentage of radiolabel. Phosphatidylinositol and phosphatidylethanolamine contained intermediate percentages while phosphatidylserine and sphingomyelin contained the least. In a dual label supplementation regimen (150 (mu)M ('14)C-EPA and 16 (mu)M ('3)H-AA), phosphatidylinositol appeared to be readily saturated with fatty acid at an early stage. This may be an important regulatory aspect for prostaglandin synthesis.","abstract_html":"An endothelial cell culture system was used to compare the effects of endogenous arachidonic acid (AA) and eicosapentaenoic acid (EPA) on prostacyclin synthesis. Supplemental levels of fatty acids (20-150 (mu)M) were supplied as sodium salts bound to bovine serum albumin (3:1 mole ratio) and added to M-199 with 20% fetal bovine serum. Both AA and EPA were readily incorporated into neutral and phospholipid fractions to serve as endogenous precursors for prostacyclin (PGI(,2)) synthesis. Thrombin (.5 U/ml) was used to stimulate prostacyclin synthesis. AA enrichment did not result in an increased PGI(,2) synthesis while EPA enrichment decreased PGI(,2) synthesis as measured by radioimmunoassay to 6-keto-PGF(,1(alpha)). Radiolabelled fatty acid eicosanoid products were separated by reverse phase HPLC. Unconverted fatty acid was the major product released upon thrombin stimulation in both cases. ('3)H-AA supplemented cells also released PGI(,2) and HETE in lesser amounts while ('14)C-EPA supplemented cells released only minimal amounts of PGI(,3) and HEPE. The relative percent incorporation of radiolabelled ('3)H-AA and ('14)C-EPA into cellular lipids was determined after two-dimensional thin-layer chromatography. Neutral lipid contained the vast majority of radiolabel ((TURN)50%). Of the phospholipids, phosphatidylcholine contained the greatest percentage of radiolabel. Phosphatidylinositol and phosphatidylethanolamine contained intermediate percentages while phosphatidylserine and sphingomyelin contained the least. In a dual label supplementation regimen (150 (mu)M ('14)C-EPA and 16 (mu)M ('3)H-AA), phosphatidylinositol appeared to be readily saturated with fatty acid at an early stage. This may be an important regulatory aspect for prostaglandin synthesis.","abstract_has_math":false,"creators":["Basgall, Edward Joseph"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Food Science","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-05-13T15:44:18Z","date_published":"2015-05-13T15:44:18Z","updated_at":"2026-07-22T22:26:10Z","subjects":["Health Sciences, Nutrition"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8409859"],"render_values":[{"text":"(UMI)AAI8409859","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/77444","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Basgall, Edward Joseph"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-05-13T15:44:18Z","10000-01-01","1983"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Health Sciences, Nutrition"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/77444","(UMI)AAI8409859"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["An endothelial cell culture system was used to compare the effects of endogenous arachidonic acid (AA) and eicosapentaenoic acid (EPA) on prostacyclin synthesis. Supplemental levels of fatty acids (20-150 (mu)M) were supplied as sodium salts bound to bovine serum albumin (3:1 mole ratio) and added to M-199 with 20% fetal bovine serum. Both AA and EPA were readily incorporated into neutral and phospholipid fractions to serve as endogenous precursors for prostacyclin (PGI(,2)) synthesis. Thrombin (.5 U/ml) was used to stimulate prostacyclin synthesis. AA enrichment did not result in an increased PGI(,2) synthesis while EPA enrichment decreased PGI(,2) synthesis as measured by radioimmunoassay to 6-keto-PGF(,1(alpha)). Radiolabelled fatty acid eicosanoid products were separated by reverse phase HPLC. Unconverted fatty acid was the major product released upon thrombin stimulation in both cases. ('3)H-AA supplemented cells also released PGI(,2) and HETE in lesser amounts while ('14)C-EPA supplemented cells released only minimal amounts of PGI(,3) and HEPE. The relative percent incorporation of radiolabelled ('3)H-AA and ('14)C-EPA into cellular lipids was determined after two-dimensional thin-layer chromatography. Neutral lipid contained the vast majority of radiolabel ((TURN)50%). Of the phospholipids, phosphatidylcholine contained the greatest percentage of radiolabel. Phosphatidylinositol and phosphatidylethanolamine contained intermediate percentages while phosphatidylserine and sphingomyelin contained the least. In a dual label supplementation regimen (150 (mu)M ('14)C-EPA and 16 (mu)M ('3)H-AA), phosphatidylinositol appeared to be readily saturated with fatty acid at an early stage. This may be an important regulatory aspect for prostaglandin synthesis.","Made available in DSpace on 2015-05-13T15:44:18Z (GMT). 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Supplemental levels of fatty acids (20-150 (mu)M) were supplied as sodium salts bound to bovine serum albumin (3:1 mole ratio) and added to M-199 with 20% fetal bovine serum. Both AA and EPA were readily incorporated into neutral and phospholipid fractions to serve as endogenous precursors for prostacyclin (PGI(,2)) synthesis. Thrombin (.5 U/ml) was used to stimulate prostacyclin synthesis. AA enrichment did not result in an increased PGI(,2) synthesis while EPA enrichment decreased PGI(,2) synthesis as measured by radioimmunoassay to 6-keto-PGF(,1(alpha)). Radiolabelled fatty acid eicosanoid products were separated by reverse phase HPLC. Unconverted fatty acid was the major product released upon thrombin stimulation in both cases. ('3)H-AA supplemented cells also released PGI(,2) and HETE in lesser amounts while ('14)C-EPA supplemented cells released only minimal amounts of PGI(,3) and HEPE. The relative percent incorporation of radiolabelled ('3)H-AA and ('14)C-EPA into cellular lipids was determined after two-dimensional thin-layer chromatography. Neutral lipid contained the vast majority of radiolabel ((TURN)50%). Of the phospholipids, phosphatidylcholine contained the greatest percentage of radiolabel. Phosphatidylinositol and phosphatidylethanolamine contained intermediate percentages while phosphatidylserine and sphingomyelin contained the least. In a dual label supplementation regimen (150 (mu)M ('14)C-EPA and 16 (mu)M ('3)H-AA), phosphatidylinositol appeared to be readily saturated with fatty acid at an early stage. This may be an important regulatory aspect for prostaglandin synthesis.","Made available in DSpace on 2015-05-13T15:44:18Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 8409859.PDF: 4495746 bytes, checksum: 418b891588ba19130cfaa1bb803bb968 (MD5) Previous issue date: 1983","Embargo set by: Seth Robbins for item 78655 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","153 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1983."],"dc:identifier":["http://hdl.handle.net/2142/77444","(UMI)AAI8409859"],"dc:language":["eng"],"dc:subject":["Health Sciences, Nutrition"],"dc:title":["Effect of Fatty Acid Modification on Prostacyclin Production in Cultured Human Endothelial Cells (Arachidonate, Elcosapentaenoate)"],"dc:type":["text"],"thesis:degree_discipline":["Food Science"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:10Z"}