{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/72836"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/72836","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Towards cloning of a novel SCN resistance locus using de novo genome assembly and fosmid screening","abstract":"Glycine soja, the wild annual ancestor of soybean, has great potential to be used as a source of genetic diversity for the crop. A Glycine soja line, PI 468916, contains a resistance QTL to soybean cyst nematode (SCN) Heterodera glycines, a devastating pest of soybean. This resistance QTL has been fine mapped in PI 468916 to a distance of 147kb. Through the use of whole genome sequencing with de novo assembly we were able to gain information about the region. In this thesis the assembly data was used along with fosmid sequences from both Sanger sequencing and Illumina reads to gain insight into sequence differences between the Williams 82 reference genome and PI 468916 to identify the gene(s) responsible for this resistance. The alignment of PI 468916 sequence against the reference genome corresponding to the resistance QTL and the candidate genes present in this region, is presented. This novel resistance locus could be used to introduce new resistance in the crop.","abstract_html":"Glycine soja, the wild annual ancestor of soybean, has great potential to be used as a source of genetic diversity for the crop. A Glycine soja line, PI 468916, contains a resistance QTL to soybean cyst nematode (SCN) Heterodera glycines, a devastating pest of soybean. This resistance QTL has been fine mapped in PI 468916 to a distance of 147kb. Through the use of whole genome sequencing with de novo assembly we were able to gain information about the region. In this thesis the assembly data was used along with fosmid sequences from both Sanger sequencing and Illumina reads to gain insight into sequence differences between the Williams 82 reference genome and PI 468916 to identify the gene(s) responsible for this resistance. The alignment of PI 468916 sequence against the reference genome corresponding to the resistance QTL and the candidate genes present in this region, is presented. This novel resistance locus could be used to introduce new resistance in the crop.","abstract_has_math":false,"creators":["Fliege, Christina"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Crop Sciences","degree_department":null,"school":null,"contributors":["Hudson, Matthew E."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-01-21T19:48:42Z","date_published":"2015-01-21T19:48:42Z","updated_at":"2026-07-22T22:26:07Z","subjects":["soybean","soybean cyst nematode (SCN)"],"languages":["en"],"rights":["Copyright 2014 Christina Fliege"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/72836","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Hudson, Matthew E."]},{"key":"dc:creator","label":"Author","values":["Fliege, Christina"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-01-21T19:48:42Z","2014-12","2015-01-21"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["soybean","soybean cyst nematode (SCN)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2014 Christina Fliege"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/72836"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Glycine soja, the wild annual ancestor of soybean, has great potential to be used as a source of genetic diversity for the crop. 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This novel resistance locus could be used to introduce new resistance in the crop.","Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2014-12-10T22:38:01Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Fliege_Christina.pdf: 1403134 bytes, checksum: ca88ad879b12cb83b42735c2562a6060 (MD5)","Made available in DSpace on 2015-01-21T19:48:42Z (GMT). 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