{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/72771"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/72771","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Design, synthesis and characterization of fluorescent hyperbranched polyglycerols and organic nanoparticles with covalently bound anti-fading agents","abstract":"The photobleaching of fluorophores is a major limitation in fluorescence microscopy. We sought to create a brighter and more stable fluorophore by covalently attaching fluorescein and anti-fading agent (AFA) moieties to hyperbranched polyglycerols (HPG). The use of HPGs as a scaffold to link fluorescein and anti-fading agents proved intractable, so a norbornyl based ROMP polymer was used instead. Through the iterative use of ROMP and RCM, organic nanoparticles (ONPs) were synthesized incorporating multiple fluorescein and AFA units. The ONPs were brighter and more photostable than fluorescein. The photostability of the ONPs without AFAs was found to loosely correlate to its brightness. Incorporation of AFAs in the ONPs led to increased stability in some cases.","abstract_html":"The photobleaching of fluorophores is a major limitation in fluorescence microscopy. We sought to create a brighter and more stable fluorophore by covalently attaching fluorescein and anti-fading agent (AFA) moieties to hyperbranched polyglycerols (HPG). The use of HPGs as a scaffold to link fluorescein and anti-fading agents proved intractable, so a norbornyl based ROMP polymer was used instead. Through the iterative use of ROMP and RCM, organic nanoparticles (ONPs) were synthesized incorporating multiple fluorescein and AFA units. The ONPs were brighter and more photostable than fluorescein. The photostability of the ONPs without AFAs was found to loosely correlate to its brightness. Incorporation of AFAs in the ONPs led to increased stability in some cases.","abstract_has_math":false,"creators":["Vincil, Gretchen"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Zimmerman, Steven C.","Hull, Kami L.","Mitchell, Douglas A.","Murphy, Catherine J."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-01-21T19:48:00Z","date_published":"2015-01-21T19:48:00Z","updated_at":"2026-07-22T22:26:07Z","subjects":["photobleaching","photostability","fluorescein","anti-fading agents","hyper-branched polymers"],"languages":["en"],"rights":["Copyright 2014 Gretchen Vincil"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/72771","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Zimmerman, Steven C.","Hull, Kami L.","Mitchell, Douglas A.","Murphy, Catherine J."]},{"key":"dc:creator","label":"Author","values":["Vincil, Gretchen"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-01-21T19:48:00Z","2014-12","2015-01-21"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["photobleaching","photostability","fluorescein","anti-fading agents","hyper-branched polymers"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2014 Gretchen Vincil"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/72771"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The photobleaching of fluorophores is a major limitation in fluorescence microscopy. We sought to create a brighter and more stable fluorophore by covalently attaching fluorescein and anti-fading agent (AFA) moieties to hyperbranched polyglycerols (HPG). The use of HPGs as a scaffold to link fluorescein and anti-fading agents proved intractable, so a norbornyl based ROMP polymer was used instead. Through the iterative use of ROMP and RCM, organic nanoparticles (ONPs) were synthesized incorporating multiple fluorescein and AFA units. The ONPs were brighter and more photostable than fluorescein. The photostability of the ONPs without AFAs was found to loosely correlate to its brightness. Incorporation of AFAs in the ONPs led to increased stability in some cases.","Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2014-12-03T16:08:50Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Vincil_Gretchen .pdf: 42839158 bytes, checksum: 8ea85715cf1d25d773720a8bce873892 (MD5)","Made available in DSpace on 2015-01-21T19:48:00Z (GMT). No. of bitstreams: 1 Gretchen_Vincil.pdf: 42849042 bytes, checksum: fe1393e7eaf5b6fc84f9ed61abf8ae82 (MD5)"]},{"key":"dc:title","label":"Title","values":["Design, synthesis and characterization of fluorescent hyperbranched polyglycerols and organic nanoparticles with covalently bound anti-fading agents"]}]}],"canonical_facts":{"dc:contributor":["Zimmerman, Steven C.","Hull, Kami L.","Mitchell, Douglas A.","Murphy, Catherine J."],"dc:creator":["Vincil, Gretchen"],"dc:date":["2015-01-21T19:48:00Z","2014-12","2015-01-21"],"dc:description":["The photobleaching of fluorophores is a major limitation in fluorescence microscopy. We sought to create a brighter and more stable fluorophore by covalently attaching fluorescein and anti-fading agent (AFA) moieties to hyperbranched polyglycerols (HPG). The use of HPGs as a scaffold to link fluorescein and anti-fading agents proved intractable, so a norbornyl based ROMP polymer was used instead. Through the iterative use of ROMP and RCM, organic nanoparticles (ONPs) were synthesized incorporating multiple fluorescein and AFA units. The ONPs were brighter and more photostable than fluorescein. The photostability of the ONPs without AFAs was found to loosely correlate to its brightness. Incorporation of AFAs in the ONPs led to increased stability in some cases.","Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2014-12-03T16:08:50Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Vincil_Gretchen .pdf: 42839158 bytes, checksum: 8ea85715cf1d25d773720a8bce873892 (MD5)","Made available in DSpace on 2015-01-21T19:48:00Z (GMT). No. of bitstreams: 1 Gretchen_Vincil.pdf: 42849042 bytes, checksum: fe1393e7eaf5b6fc84f9ed61abf8ae82 (MD5)"],"dc:identifier":["http://hdl.handle.net/2142/72771"],"dc:language":["en"],"dc:rights":["Copyright 2014 Gretchen Vincil"],"dc:subject":["photobleaching","photostability","fluorescein","anti-fading agents","hyper-branched polymers"],"dc:title":["Design, synthesis and characterization of fluorescent hyperbranched polyglycerols and organic nanoparticles with covalently bound anti-fading agents"],"dc:type":["text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:07Z"}