{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/72624"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/72624","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Investigations Into the Effects of Light and Darkness on Chlorophyll Biosynthesis in Angiosperms (Monovinyl, Divinyl Protochlorophyllide)","abstract":"Three separate studies were undertaken.","abstract_html":"Three separate studies were undertaken.","abstract_has_math":false,"creators":["Carey, Edward Ewing"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Horticulture","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-17T23:57:13Z","date_published":"2014-12-17T23:57:13Z","updated_at":"2026-07-22T22:26:07Z","subjects":["Biology, Plant Physiology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8521730"],"render_values":[{"text":"(UMI)AAI8521730","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/72624","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Carey, Edward Ewing"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-17T23:57:13Z","10000-01-01","1985"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Horticulture"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Plant Physiology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/72624","(UMI)AAI8521730"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Three separate studies were undertaken.","In the first study, changes in the monovinyl (MV) and divinyl (DV) protochlorophyllide (Pchlide) contents of photoperiodically grown seedlings of nine dicotyledonous and four monocotyledonous species were followed during periods of darkness and light. DV Pchlide was predominant in all species at the end of a 14 hour light period. During a 10 h dark period, MV Pchlide accumulated in all species. In some species DV Pchlide also accumulated, while in others its levels fell. When plants were placed in the light at the end of the dark period, Pchlide levels fell, due to photoconversion to Chlorophyllide a. In light, DV Pchlide returned to predominance. Thus, there was a differential contribution of MV and DV Pchlide to Chlorophyll biosynthesis.","In the second study, the MV and DV Pchlid biosynthetic capabilities of whole maize, barley and cucumber seedling tissues, and of etiochloroplasts isolated from them were studied. It was found that light and darkness had similar effects on Pchlide biosynthesis in etiochloroplasts in vitro and in vivo. These effects were similar to those observed in the previous study on photoperiodically grown seedlings--ie. maize synthesized mainly MV Pchlide, and cucumber synthesized both MV and DV Pchlide in darkness. Thus, species-dependent differences in the effects of darkness and light on Pchlide biosynthesis were present very early in plastid greening. Furthermore, isolated plastids possessed the ability to respond to the light and dark stimuli. The in vitro maize and barley systems developed in this work may prove to be useful in determining the biochemical basis of the effects of light and darkness on Pchlide biosynthesis.","In the third study, a ('14)C ALA labelling experiment using photoperiodically grown bean leaves, produced preliminary evidence of dark biosynthesis of a putative reaction center chlorophyll.","Made available in DSpace on 2014-12-17T23:57:13Z (GMT). No. of bitstreams: 1 8521730.pdf: 4201709 bytes, checksum: 06a3591a98ba78c22ffd930ef1f50fd7 (MD5) Previous issue date: 1985","Embargo set by: Seth Robbins for item 72792 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","129 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1985."]},{"key":"dc:title","label":"Title","values":["Investigations Into the Effects of Light and Darkness on Chlorophyll Biosynthesis in Angiosperms (Monovinyl, Divinyl Protochlorophyllide)"]}]}],"canonical_facts":{"dc:creator":["Carey, Edward Ewing"],"dc:date":["2014-12-17T23:57:13Z","10000-01-01","1985"],"dc:description":["Three separate studies were undertaken.","In the first study, changes in the monovinyl (MV) and divinyl (DV) protochlorophyllide (Pchlide) contents of photoperiodically grown seedlings of nine dicotyledonous and four monocotyledonous species were followed during periods of darkness and light. DV Pchlide was predominant in all species at the end of a 14 hour light period. During a 10 h dark period, MV Pchlide accumulated in all species. In some species DV Pchlide also accumulated, while in others its levels fell. When plants were placed in the light at the end of the dark period, Pchlide levels fell, due to photoconversion to Chlorophyllide a. In light, DV Pchlide returned to predominance. Thus, there was a differential contribution of MV and DV Pchlide to Chlorophyll biosynthesis.","In the second study, the MV and DV Pchlid biosynthetic capabilities of whole maize, barley and cucumber seedling tissues, and of etiochloroplasts isolated from them were studied. It was found that light and darkness had similar effects on Pchlide biosynthesis in etiochloroplasts in vitro and in vivo. These effects were similar to those observed in the previous study on photoperiodically grown seedlings--ie. maize synthesized mainly MV Pchlide, and cucumber synthesized both MV and DV Pchlide in darkness. Thus, species-dependent differences in the effects of darkness and light on Pchlide biosynthesis were present very early in plastid greening. Furthermore, isolated plastids possessed the ability to respond to the light and dark stimuli. The in vitro maize and barley systems developed in this work may prove to be useful in determining the biochemical basis of the effects of light and darkness on Pchlide biosynthesis.","In the third study, a ('14)C ALA labelling experiment using photoperiodically grown bean leaves, produced preliminary evidence of dark biosynthesis of a putative reaction center chlorophyll.","Made available in DSpace on 2014-12-17T23:57:13Z (GMT). No. of bitstreams: 1 8521730.pdf: 4201709 bytes, checksum: 06a3591a98ba78c22ffd930ef1f50fd7 (MD5) Previous issue date: 1985","Embargo set by: Seth Robbins for item 72792 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","129 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1985."],"dc:identifier":["http://hdl.handle.net/2142/72624","(UMI)AAI8521730"],"dc:subject":["Biology, Plant Physiology"],"dc:title":["Investigations Into the Effects of Light and Darkness on Chlorophyll Biosynthesis in Angiosperms (Monovinyl, Divinyl Protochlorophyllide)"],"dc:type":["text"],"thesis:degree_discipline":["Horticulture"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:07Z"}