{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/72554"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/72554","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The Mechanism of Cyanohydroxybutene Induced Glutathione Elevation: The Role of Gamma-Glutamylcysteine Synthetase","abstract":"Cyanohydroxybutene (CHB; 200 mg/kg po) causes a 2- and 7-fold elevation in glutathione (GSH) in liver and pancreas, respectively. The mechanism of elevation is currently unknown. In an attempt to identify the mechanism of this GSH induction, the effect of CHB on hepatic and pancreatic $\\gamma$-glutamylcysteine synthetase (GCS) activity, formation in vitro of a glutathione-S-transferase (GST) catalyzed adduct, the effect of CHB on hepatic and pancreatic cysteine equivalents (cysteine, cystine, and cysteinylglycine), and the acute effect of CHB on expression of the hepatic mRNA for $\\gamma$-glutamylcysteine synthetase (GCS) were investigated. The results of these studies suggest that the mechanism of CHB-mediated induction of GSH involves an increase in GCS mRNA producing an increase in GCS enzyme and thereby increasing GSH synthesis. This mechanism and the subsequent GSH elevation may have possible therapeutic and prophylactic implications.","abstract_html":"Cyanohydroxybutene (CHB; 200 mg/kg po) causes a 2- and 7-fold elevation in glutathione (GSH) in liver and pancreas, respectively. The mechanism of elevation is currently unknown. In an attempt to identify the mechanism of this GSH induction, the effect of CHB on hepatic and pancreatic <span class=\"etd-inline-math\">&gamma;</span>-glutamylcysteine synthetase (GCS) activity, formation in vitro of a glutathione-S-transferase (GST) catalyzed adduct, the effect of CHB on hepatic and pancreatic cysteine equivalents (cysteine, cystine, and cysteinylglycine), and the acute effect of CHB on expression of the hepatic mRNA for <span class=\"etd-inline-math\">&gamma;</span>-glutamylcysteine synthetase (GCS) were investigated. The results of these studies suggest that the mechanism of CHB-mediated induction of GSH involves an increase in GCS mRNA producing an increase in GCS enzyme and thereby increasing GSH synthesis. This mechanism and the subsequent GSH elevation may have possible therapeutic and prophylactic implications.","abstract_has_math":true,"creators":["Davis, Myrtle A."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Veterinary Medical Science","degree_department":null,"school":null,"contributors":["Jeffery, E.,"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-17T23:22:41Z","date_published":"2014-12-17T23:22:41Z","updated_at":"2026-07-22T22:26:07Z","subjects":["Health Sciences, Toxicology","Biology, Veterinary Science"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI9305502"],"render_values":[{"text":"(UMI)AAI9305502","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/72554","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Jeffery, E.,"]},{"key":"dc:creator","label":"Author","values":["Davis, Myrtle A."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-17T23:22:41Z","10000-01-01","1992"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Veterinary Medical Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Health Sciences, Toxicology","Biology, Veterinary Science"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/72554","(UMI)AAI9305502"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Cyanohydroxybutene (CHB; 200 mg/kg po) causes a 2- and 7-fold elevation in glutathione (GSH) in liver and pancreas, respectively. The mechanism of elevation is currently unknown. In an attempt to identify the mechanism of this GSH induction, the effect of CHB on hepatic and pancreatic $\\gamma$-glutamylcysteine synthetase (GCS) activity, formation in vitro of a glutathione-S-transferase (GST) catalyzed adduct, the effect of CHB on hepatic and pancreatic cysteine equivalents (cysteine, cystine, and cysteinylglycine), and the acute effect of CHB on expression of the hepatic mRNA for $\\gamma$-glutamylcysteine synthetase (GCS) were investigated. The results of these studies suggest that the mechanism of CHB-mediated induction of GSH involves an increase in GCS mRNA producing an increase in GCS enzyme and thereby increasing GSH synthesis. This mechanism and the subsequent GSH elevation may have possible therapeutic and prophylactic implications.","Made available in DSpace on 2014-12-17T23:22:41Z (GMT). No. of bitstreams: 1 9305502.pdf: 5100648 bytes, checksum: 28396b6f5df28e2b647179272839bbe9 (MD5) Previous issue date: 1992","Embargo set by: Seth Robbins for item 72722 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","138 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1992."]},{"key":"dc:title","label":"Title","values":["The Mechanism of Cyanohydroxybutene Induced Glutathione Elevation: The Role of Gamma-Glutamylcysteine Synthetase"]}]}],"canonical_facts":{"dc:contributor":["Jeffery, E.,"],"dc:creator":["Davis, Myrtle A."],"dc:date":["2014-12-17T23:22:41Z","10000-01-01","1992"],"dc:description":["Cyanohydroxybutene (CHB; 200 mg/kg po) causes a 2- and 7-fold elevation in glutathione (GSH) in liver and pancreas, respectively. The mechanism of elevation is currently unknown. In an attempt to identify the mechanism of this GSH induction, the effect of CHB on hepatic and pancreatic $\\gamma$-glutamylcysteine synthetase (GCS) activity, formation in vitro of a glutathione-S-transferase (GST) catalyzed adduct, the effect of CHB on hepatic and pancreatic cysteine equivalents (cysteine, cystine, and cysteinylglycine), and the acute effect of CHB on expression of the hepatic mRNA for $\\gamma$-glutamylcysteine synthetase (GCS) were investigated. The results of these studies suggest that the mechanism of CHB-mediated induction of GSH involves an increase in GCS mRNA producing an increase in GCS enzyme and thereby increasing GSH synthesis. This mechanism and the subsequent GSH elevation may have possible therapeutic and prophylactic implications.","Made available in DSpace on 2014-12-17T23:22:41Z (GMT). No. of bitstreams: 1 9305502.pdf: 5100648 bytes, checksum: 28396b6f5df28e2b647179272839bbe9 (MD5) Previous issue date: 1992","Embargo set by: Seth Robbins for item 72722 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","138 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1992."],"dc:identifier":["http://hdl.handle.net/2142/72554","(UMI)AAI9305502"],"dc:subject":["Health Sciences, Toxicology","Biology, Veterinary Science"],"dc:title":["The Mechanism of Cyanohydroxybutene Induced Glutathione Elevation: The Role of Gamma-Glutamylcysteine Synthetase"],"dc:type":["text"],"thesis:degree_discipline":["Veterinary Medical Science"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:07Z"}