{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/72243"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/72243","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Hybrid Micro/nanofluidic Sampling Schemes for Mass Spectrometry","abstract":"Novel analytical sampling techniques are investigated in an effort to advance the exploration of chemical reactions underlying life processes. The analysis of substances found in living organisms is challenging and includes the precise collection, preparation, and identification of complex biological samples. An appropriate sampling scheme simplifies the collection, as well as the pretreatment procedures in order to allow for more direct chemical analyses. Here, hybrid micro/nanofluidic manipulation schemes are explored and interfaced with mass spectrometry in the effort to study the distribution and dynamic release of neurotransmitters and neuropeptides from individual neurons.","abstract_html":"Novel analytical sampling techniques are investigated in an effort to advance the exploration of chemical reactions underlying life processes. The analysis of substances found in living organisms is challenging and includes the precise collection, preparation, and identification of complex biological samples. An appropriate sampling scheme simplifies the collection, as well as the pretreatment procedures in order to allow for more direct chemical analyses. Here, hybrid micro/nanofluidic manipulation schemes are explored and interfaced with mass spectrometry in the effort to study the distribution and dynamic release of neurotransmitters and neuropeptides from individual neurons.","abstract_has_math":false,"creators":["Iannacone, Jamie Marie"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Sweedler, Jonathan V."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-17T21:28:46Z","date_published":"2014-12-17T21:28:46Z","updated_at":"2026-07-22T22:26:06Z","subjects":["Chemistry, Analytical"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI3314801"],"render_values":[{"text":"(UMI)AAI3314801","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/72243","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Sweedler, Jonathan V."]},{"key":"dc:creator","label":"Author","values":["Iannacone, Jamie Marie"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-17T21:28:46Z","10000-01-01","2008"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Analytical"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/72243","(UMI)AAI3314801"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Novel analytical sampling techniques are investigated in an effort to advance the exploration of chemical reactions underlying life processes. The analysis of substances found in living organisms is challenging and includes the precise collection, preparation, and identification of complex biological samples. An appropriate sampling scheme simplifies the collection, as well as the pretreatment procedures in order to allow for more direct chemical analyses. Here, hybrid micro/nanofluidic manipulation schemes are explored and interfaced with mass spectrometry in the effort to study the distribution and dynamic release of neurotransmitters and neuropeptides from individual neurons.","Molecular gate devices are composed of spatially isolated microfluidic channels connected via nanocapillary array membranes. The hybrid micro/nanofluidic tools are fabricated with poly(dimethylsiloxane) or poly(methyl methacrylate) in the presence of a polycarbonate membrane. Chips composed of each polymer substrate are successfully interfaced to electrospray ionization mass spectrometry, as stable electrosprays and high-quality mass spectra are generated. Furthermore, the poly(dimethylsiloxane)-based microchip emitter is used for on-line sample treatment prior to detection.","Porous polymer monoliths offer efficient mass transfer and high permeability as the nanoporous backbone interconnects to form multiple microfluidic flow-through channels. More commonly used as chromatographic media, the monoliths are investigated for their potential use as novel sampling tools. Fabricated in a capillary holder, porous polymer monoliths are interfaced with both electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry. The direct collection and subsequent detection of complex biological samples is further realized with the analysis of Aplysia californica bag cell releasate using matrix-assisted laser desorption/ionization mass spectrometry.","Made available in DSpace on 2014-12-17T21:28:46Z (GMT). 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The analysis of substances found in living organisms is challenging and includes the precise collection, preparation, and identification of complex biological samples. An appropriate sampling scheme simplifies the collection, as well as the pretreatment procedures in order to allow for more direct chemical analyses. Here, hybrid micro/nanofluidic manipulation schemes are explored and interfaced with mass spectrometry in the effort to study the distribution and dynamic release of neurotransmitters and neuropeptides from individual neurons.","Molecular gate devices are composed of spatially isolated microfluidic channels connected via nanocapillary array membranes. The hybrid micro/nanofluidic tools are fabricated with poly(dimethylsiloxane) or poly(methyl methacrylate) in the presence of a polycarbonate membrane. Chips composed of each polymer substrate are successfully interfaced to electrospray ionization mass spectrometry, as stable electrosprays and high-quality mass spectra are generated. Furthermore, the poly(dimethylsiloxane)-based microchip emitter is used for on-line sample treatment prior to detection.","Porous polymer monoliths offer efficient mass transfer and high permeability as the nanoporous backbone interconnects to form multiple microfluidic flow-through channels. More commonly used as chromatographic media, the monoliths are investigated for their potential use as novel sampling tools. Fabricated in a capillary holder, porous polymer monoliths are interfaced with both electrospray ionization and matrix-assisted laser desorption/ionization mass spectrometry. The direct collection and subsequent detection of complex biological samples is further realized with the analysis of Aplysia californica bag cell releasate using matrix-assisted laser desorption/ionization mass spectrometry.","Made available in DSpace on 2014-12-17T21:28:46Z (GMT). No. of bitstreams: 1 3314801.pdf: 2811289 bytes, checksum: 7f81b9d31037ae1813760d7d7cf85423 (MD5) Previous issue date: 2008","Embargo set by: Seth Robbins for item 72411 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","173 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008."],"dc:identifier":["http://hdl.handle.net/2142/72243","(UMI)AAI3314801"],"dc:subject":["Chemistry, Analytical"],"dc:title":["Hybrid Micro/nanofluidic Sampling Schemes for Mass Spectrometry"],"dc:type":["text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:06Z"}