Regulation of Ammonia Assimilating Enzymes in the Ruminal Bacteria Selenomonas Ruminantium and Succinivibrio Dextrinosolvens
Selenomonas ruminantium was grown in continuous culture under glucose-limitation at dilution rates of .05, .10, .20, .30 and .42 and under ammonia-limitation at dilution rates of .05, .10 and .20, to determine the effects of dilution rate and growth-limiting nutrients on cell physiology, efficiency of growth and ammonia assimilating enzymes. Culture viability was decreased at low dilution rates for both growth-limiting conditions, but the decrease in viability was more severe in glucose-limited cultures. Cell composition changed little under the conditions tested; however, the amount of nitrogen containing material (as % dry weight) was lower in ammonia-limited than in glucose-limited cultures of S. ruminantium.
Agriculture, Animal Culture and Nutrition
University of Illinois - Urbana-Champaign
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University of Illinois at Urbana-Champaign