{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/71617"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/71617","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The Genetics of Soybean Seed Urease (Isozymes, Clycine Max, Transcription)","abstract":"The objectives were (i) to study the inheritance of the soybean seed urease isozymes, (ii) to determine the cause of the isozyme polymorphism and (iii) to study the inheritance of a soybean seed urease null.","abstract_html":"The objectives were (i) to study the inheritance of the soybean seed urease isozymes, (ii) to determine the cause of the isozyme polymorphism and (iii) to study the inheritance of a soybean seed urease null.","abstract_has_math":false,"creators":["Kloth, Reiner Hans"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Agronomy","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-16T19:03:33Z","date_published":"2014-12-16T19:03:33Z","updated_at":"2026-07-22T22:26:05Z","subjects":["Biology, Genetics"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8600243"],"render_values":[{"text":"(UMI)AAI8600243","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/71617","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Kloth, Reiner Hans"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-16T19:03:33Z","10000-01-01","1985"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Agronomy"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Genetics"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/71617","(UMI)AAI8600243"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The objectives were (i) to study the inheritance of the soybean seed urease isozymes, (ii) to determine the cause of the isozyme polymorphism and (iii) to study the inheritance of a soybean seed urease null.","Reciprocal crosses between cultivars homozygous for the electrophoretic slow and fast forms of seed urease were made. The results indicate that the fast and slow variants are inherited as codominant alleles.","Before begining a study to determine the source of the polymorphism between the fast and slow forms of seed urease, a speedier means of purifying urease is necessary. Seed urease was adsorbed to chloramphenicol-caproate substituted agarose. The enzyme was eluted when the ionic strength of the solution was increased and the pH decreased. Preliminary experiments show a thousand-fold purification of urease with a low yield.","The soybean seed urease null phenotype was previously described as lacking seed urease activity and antigen. Null lines behave similarly when crossed to cultivars homozygous for the slow or fast alleles of seed urease: the F(,1) seed from crosses of urease expressing cultivars with nulls express the urease allele of the cultivar and a 3:1 segregation for the presence and absence of urease is observed in progeny from F(,1) and heterozygous F(,2) plants. Examination of F(,2) and F(,3) urease positive seed revealed that this seed from fast x null crosses are all phenotypically urease fast, while the same seed from slow x null crosses shows a segregation of one seed that contains a fast urease either exclusively or in a heterozygous state with the slow isozyme for every 69 phenotypic slows. The data indicates that the urease null is caused by a cis-acting, epistatic allele linked to, but distinct from the isozyme locus. The urease nulls all carry an unexpressed fast form of seed urease.","Made available in DSpace on 2014-12-16T19:03:33Z (GMT). No. of bitstreams: 1 8600243.pdf: 2191194 bytes, checksum: 809f06702ebc43ba4abeb1e1f59e1e28 (MD5) Previous issue date: 1985","Embargo set by: Seth Robbins for item 71783 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community indefinitely during batch ingest of legacy ETDs","U of I Only","79 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1985."]},{"key":"dc:title","label":"Title","values":["The Genetics of Soybean Seed Urease (Isozymes, Clycine Max, Transcription)"]}]}],"canonical_facts":{"dc:creator":["Kloth, Reiner Hans"],"dc:date":["2014-12-16T19:03:33Z","10000-01-01","1985"],"dc:description":["The objectives were (i) to study the inheritance of the soybean seed urease isozymes, (ii) to determine the cause of the isozyme polymorphism and (iii) to study the inheritance of a soybean seed urease null.","Reciprocal crosses between cultivars homozygous for the electrophoretic slow and fast forms of seed urease were made. The results indicate that the fast and slow variants are inherited as codominant alleles.","Before begining a study to determine the source of the polymorphism between the fast and slow forms of seed urease, a speedier means of purifying urease is necessary. Seed urease was adsorbed to chloramphenicol-caproate substituted agarose. The enzyme was eluted when the ionic strength of the solution was increased and the pH decreased. Preliminary experiments show a thousand-fold purification of urease with a low yield.","The soybean seed urease null phenotype was previously described as lacking seed urease activity and antigen. Null lines behave similarly when crossed to cultivars homozygous for the slow or fast alleles of seed urease: the F(,1) seed from crosses of urease expressing cultivars with nulls express the urease allele of the cultivar and a 3:1 segregation for the presence and absence of urease is observed in progeny from F(,1) and heterozygous F(,2) plants. Examination of F(,2) and F(,3) urease positive seed revealed that this seed from fast x null crosses are all phenotypically urease fast, while the same seed from slow x null crosses shows a segregation of one seed that contains a fast urease either exclusively or in a heterozygous state with the slow isozyme for every 69 phenotypic slows. The data indicates that the urease null is caused by a cis-acting, epistatic allele linked to, but distinct from the isozyme locus. The urease nulls all carry an unexpressed fast form of seed urease.","Made available in DSpace on 2014-12-16T19:03:33Z (GMT). No. of bitstreams: 1 8600243.pdf: 2191194 bytes, checksum: 809f06702ebc43ba4abeb1e1f59e1e28 (MD5) Previous issue date: 1985","Embargo set by: Seth Robbins for item 71783 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community indefinitely during batch ingest of legacy ETDs","U of I Only","79 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1985."],"dc:identifier":["http://hdl.handle.net/2142/71617","(UMI)AAI8600243"],"dc:subject":["Biology, Genetics"],"dc:title":["The Genetics of Soybean Seed Urease (Isozymes, Clycine Max, Transcription)"],"dc:type":["text"],"thesis:degree_discipline":["Agronomy"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:05Z"}