{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/71597"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/71597","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Evolution of Nitrogen Compounds From Soybean (Acetaldehyde Oxime, Nitrous Oxide, N-15)","abstract":"Soybean seedlings {Glycine max (L.) Merr. cv. Williams, 24 days after planting, grown on nutrient solution containing 15 mM NO(,3)('-)} evolved substantial amounts of N compounds in an open gas exchange system. Air exiting the shoot compartment was passed through a chemical trapping system. Traps I, II, and III contained 0.1 N H(,2)SO(,4), 1 N KOH, and 1 N KOH:0.1 M KMnO(,4), respectively. The N content of the trapping solutions was determined by steam distillation methods. Over a 24 h period, the shoots evolved 840 (mu)g N(.)plant('-1)(.)d('-1). Maximum evolution rates occurred near the end of the light period. Trap II retained 62.1% of the N evolved, whereas traps I and III retained 18.7% and 19.2%, respectively, suggesting that the bulk of the N compounds evolved were acidic. Ammonia plus amines (trap I) comprised less than 105 of the N evolved. Substantial amounts of oxidized inorganic N were detected in the traps, indicating that part of the N evolved was more oxidized than NH(,3). Evolution of N compounds detected as NO(,x) was also observed (mean rate for 24 h was 0.57 (mu)g N(.)plant('-1)(.)h('-1)). The results of hydrolysis experiments (acidic and alkaline conditions) indicate that part of the N evolved was organic.","abstract_html":"Soybean seedlings {Glycine max (L.) Merr. cv. Williams, 24 days after planting, grown on nutrient solution containing 15 mM NO(,3)('-)} evolved substantial amounts of N compounds in an open gas exchange system. Air exiting the shoot compartment was passed through a chemical trapping system. Traps I, II, and III contained 0.1 N H(,2)SO(,4), 1 N KOH, and 1 N KOH:0.1 M KMnO(,4), respectively. The N content of the trapping solutions was determined by steam distillation methods. Over a 24 h period, the shoots evolved 840 (mu)g N(.)plant('-1)(.)d('-1). Maximum evolution rates occurred near the end of the light period. Trap II retained 62.1% of the N evolved, whereas traps I and III retained 18.7% and 19.2%, respectively, suggesting that the bulk of the N compounds evolved were acidic. Ammonia plus amines (trap I) comprised less than 105 of the N evolved. Substantial amounts of oxidized inorganic N were detected in the traps, indicating that part of the N evolved was more oxidized than NH(,3). Evolution of N compounds detected as NO(,x) was also observed (mean rate for 24 h was 0.57 (mu)g N(.)plant('-1)(.)h('-1)). The results of hydrolysis experiments (acidic and alkaline conditions) indicate that part of the N evolved was organic.","abstract_has_math":false,"creators":["Mulvaney, Charlene Steele"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Agronomy","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-16T19:03:25Z","date_published":"2014-12-16T19:03:25Z","updated_at":"2026-07-22T22:26:05Z","subjects":["Biology, Plant Physiology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8422783"],"render_values":[{"text":"(UMI)AAI8422783","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/71597","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Mulvaney, Charlene Steele"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-16T19:03:25Z","10000-01-01","1984"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Agronomy"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Plant Physiology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/71597","(UMI)AAI8422783"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Soybean seedlings {Glycine max (L.) Merr. cv. Williams, 24 days after planting, grown on nutrient solution containing 15 mM NO(,3)('-)} evolved substantial amounts of N compounds in an open gas exchange system. Air exiting the shoot compartment was passed through a chemical trapping system. Traps I, II, and III contained 0.1 N H(,2)SO(,4), 1 N KOH, and 1 N KOH:0.1 M KMnO(,4), respectively. The N content of the trapping solutions was determined by steam distillation methods. Over a 24 h period, the shoots evolved 840 (mu)g N(.)plant('-1)(.)d('-1). Maximum evolution rates occurred near the end of the light period. Trap II retained 62.1% of the N evolved, whereas traps I and III retained 18.7% and 19.2%, respectively, suggesting that the bulk of the N compounds evolved were acidic. Ammonia plus amines (trap I) comprised less than 105 of the N evolved. Substantial amounts of oxidized inorganic N were detected in the traps, indicating that part of the N evolved was more oxidized than NH(,3). Evolution of N compounds detected as NO(,x) was also observed (mean rate for 24 h was 0.57 (mu)g N(.)plant('-1)(.)h('-1)). The results of hydrolysis experiments (acidic and alkaline conditions) indicate that part of the N evolved was organic.","Evolution of nitrogen oxides {NO(,x), primarily as nitric oxide (NO)} from soybean leaves during purged in vivo nitrate reductase assays was reported, however these reports were based on a method for NO(,x) determination in air. This method also detects other N compounds. Preliminary work led us to doubt that the N evolved was NO. Studies were undertaken to identify the N compound evolved from the in vivo assay that was reported as NO(,x). Material for identification was obtained by cryogenic trapping procedures. Mass spectrometry, ultraviolet spectroscopy, and ('15)NO(,3)('-) were used to identify the compounds evolved and to determine whether the compounds were derived from nitrate. Acetaldehyde oxime was identified as the predominant N compound evolved, and is readily detected by the method for NO(,x). Substantial quantities of acetaldehyde oxime were evolved during the in vivo assay (16.2 (mu)mol(.)g('-1) fresh weight(.)h('-1)). Small amounts of nitrous oxide (N(,2)O) were evolved (0.63 (mu)g N(.)g('-1) fresh weight(.)h('-1)), but N(,2)O was not detected as NO(,x). Acetaldehyde oxime and N(,2)O were both produced as a result of ('15)NO(,3)('-) reduction during the assay.","Made available in DSpace on 2014-12-16T19:03:25Z (GMT). No. of bitstreams: 1 8422783.pdf: 2323498 bytes, checksum: c8d98dab5f9a4eaa46764cdcd4c7e92c (MD5) Previous issue date: 1984","Embargo set by: Seth Robbins for item 71763 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community indefinitely during batch ingest of legacy ETDs","U of I Only","74 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1984."]},{"key":"dc:title","label":"Title","values":["Evolution of Nitrogen Compounds From Soybean (Acetaldehyde Oxime, Nitrous Oxide, N-15)"]}]}],"canonical_facts":{"dc:creator":["Mulvaney, Charlene Steele"],"dc:date":["2014-12-16T19:03:25Z","10000-01-01","1984"],"dc:description":["Soybean seedlings {Glycine max (L.) Merr. cv. Williams, 24 days after planting, grown on nutrient solution containing 15 mM NO(,3)('-)} evolved substantial amounts of N compounds in an open gas exchange system. Air exiting the shoot compartment was passed through a chemical trapping system. Traps I, II, and III contained 0.1 N H(,2)SO(,4), 1 N KOH, and 1 N KOH:0.1 M KMnO(,4), respectively. The N content of the trapping solutions was determined by steam distillation methods. Over a 24 h period, the shoots evolved 840 (mu)g N(.)plant('-1)(.)d('-1). Maximum evolution rates occurred near the end of the light period. Trap II retained 62.1% of the N evolved, whereas traps I and III retained 18.7% and 19.2%, respectively, suggesting that the bulk of the N compounds evolved were acidic. Ammonia plus amines (trap I) comprised less than 105 of the N evolved. Substantial amounts of oxidized inorganic N were detected in the traps, indicating that part of the N evolved was more oxidized than NH(,3). Evolution of N compounds detected as NO(,x) was also observed (mean rate for 24 h was 0.57 (mu)g N(.)plant('-1)(.)h('-1)). The results of hydrolysis experiments (acidic and alkaline conditions) indicate that part of the N evolved was organic.","Evolution of nitrogen oxides {NO(,x), primarily as nitric oxide (NO)} from soybean leaves during purged in vivo nitrate reductase assays was reported, however these reports were based on a method for NO(,x) determination in air. This method also detects other N compounds. Preliminary work led us to doubt that the N evolved was NO. Studies were undertaken to identify the N compound evolved from the in vivo assay that was reported as NO(,x). Material for identification was obtained by cryogenic trapping procedures. Mass spectrometry, ultraviolet spectroscopy, and ('15)NO(,3)('-) were used to identify the compounds evolved and to determine whether the compounds were derived from nitrate. Acetaldehyde oxime was identified as the predominant N compound evolved, and is readily detected by the method for NO(,x). Substantial quantities of acetaldehyde oxime were evolved during the in vivo assay (16.2 (mu)mol(.)g('-1) fresh weight(.)h('-1)). Small amounts of nitrous oxide (N(,2)O) were evolved (0.63 (mu)g N(.)g('-1) fresh weight(.)h('-1)), but N(,2)O was not detected as NO(,x). Acetaldehyde oxime and N(,2)O were both produced as a result of ('15)NO(,3)('-) reduction during the assay.","Made available in DSpace on 2014-12-16T19:03:25Z (GMT). No. of bitstreams: 1 8422783.pdf: 2323498 bytes, checksum: c8d98dab5f9a4eaa46764cdcd4c7e92c (MD5) Previous issue date: 1984","Embargo set by: Seth Robbins for item 71763 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community indefinitely during batch ingest of legacy ETDs","U of I Only","74 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1984."],"dc:identifier":["http://hdl.handle.net/2142/71597","(UMI)AAI8422783"],"dc:subject":["Biology, Plant Physiology"],"dc:title":["Evolution of Nitrogen Compounds From Soybean (Acetaldehyde Oxime, Nitrous Oxide, N-15)"],"dc:type":["text"],"thesis:degree_discipline":["Agronomy"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:05Z"}