{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/71455"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/71455","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The Role of Potassium as a Counterion During Calcium Release From the Sarcoplasmic Reticulum","abstract":"There is great interest in the effect of Ca$\\sp{2+}$ release on the electrical properties of the sarcoplasmic reticulum (SR) membranes since a voltage change may play an important role in muscle regulation. Hence the role of K$\\sp{+}$ as a counterion during Ca$\\sp{2+}$ release from the SR was investigated and found to be physiologically important.","abstract_html":"There is great interest in the effect of Ca$\\sp{2+}$ release on the electrical properties of the sarcoplasmic reticulum (SR) membranes since a voltage change may play an important role in muscle regulation. Hence the role of K$\\sp{+}$ as a counterion during Ca$\\sp{2+}$ release from the SR was investigated and found to be physiologically important.","abstract_has_math":true,"creators":["Abramcheck, Carla Wilt"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Physiology","degree_department":null,"school":null,"contributors":["Best, Philip M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-16T06:45:54Z","date_published":"2014-12-16T06:45:54Z","updated_at":"2026-07-22T22:26:04Z","subjects":["Biology, Animal Physiology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8823068"],"render_values":[{"text":"(UMI)AAI8823068","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/71455","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Best, Philip M."]},{"key":"dc:creator","label":"Author","values":["Abramcheck, Carla Wilt"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-16T06:45:54Z","10000-01-01","1988"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Animal Physiology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/71455","(UMI)AAI8823068"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["There is great interest in the effect of Ca$\\sp{2+}$ release on the electrical properties of the sarcoplasmic reticulum (SR) membranes since a voltage change may play an important role in muscle regulation. Hence the role of K$\\sp{+}$ as a counterion during Ca$\\sp{2+}$ release from the SR was investigated and found to be physiologically important.","The resting K$\\sp{+}$ permeability of the SR membrane was determined from the passive efflux of $\\sp{42}$K from single skinned skeletal muscle fibers. The efflux curves were kinetically complex and were fit as the sum of three exponentials. In experiments using pretreatment with detergent or $\\sp $C-sucrose the efflux curve lacked the intermediate component. Therefore we concluded that the intermediate component represented movement across a membrane-bound space with a relative fiber volume equivalent to that of the SR. Our estimate of the in situ K$\\sp{+}$ permeability for the SR based on the efflux data is 10$\\sp{-7}$ cm/sec and is sufficient to support a large K$\\sp{+}$ counterion flux.","The effect of a decreased SR K$\\sp{+}$ conductance on the early Ca$\\sp{2+}$ release rate was investigated. An optical technique using the Ca$\\sp{2+}$-sensitive dye Antipyrylazo III was used to monitor caffeine stimulated Ca$\\sp{2+}$ release from skinned skeletal muscle fibers. The SR K$\\sp{+}$ channel blocker bisG10 and substitution of the impermeant ion choline for K$\\sp{+}$ were used as two different methods to decrease SR K$\\sp{+}$ conductance. Both methods caused a concentration dependent decrease in the Ca$\\sp{2+}$ release rate. Therefore we concluded that K$\\sp{+}$ is a counterion for Ca$\\sp{2+}$ during its release from the SR.","The optical technique was then used to determine the selectivity sequence of the in situ SR K$\\sp{+}$ channel to several monovalent cations by substituting them for K$\\sp{+}$. These ions will effect Ca$\\sp{+}$ release dependent on their ability to support counterion flux which is a function of their relative conductance to the SR K$\\sp{+}$ channel. The selectivity sequence determined by these experiments was: K$\\sp{+}$ = Rb$\\sp{+}$ = Na$\\sp{+}$ $>$ Cs$\\sp{+}$ $>$ Li$\\sp{+}$ $>$ choline.","Made available in DSpace on 2014-12-16T06:45:54Z (GMT). No. of bitstreams: 1 8823068.pdf: 3309788 bytes, checksum: d2c644f146b48cf3e19af415350f7132 (MD5) Previous issue date: 1988","Embargo set by: Seth Robbins for item 71621 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","100 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1988."]},{"key":"dc:title","label":"Title","values":["The Role of Potassium as a Counterion During Calcium Release From the Sarcoplasmic Reticulum"]}]}],"canonical_facts":{"dc:contributor":["Best, Philip M."],"dc:creator":["Abramcheck, Carla Wilt"],"dc:date":["2014-12-16T06:45:54Z","10000-01-01","1988"],"dc:description":["There is great interest in the effect of Ca$\\sp{2+}$ release on the electrical properties of the sarcoplasmic reticulum (SR) membranes since a voltage change may play an important role in muscle regulation. Hence the role of K$\\sp{+}$ as a counterion during Ca$\\sp{2+}$ release from the SR was investigated and found to be physiologically important.","The resting K$\\sp{+}$ permeability of the SR membrane was determined from the passive efflux of $\\sp{42}$K from single skinned skeletal muscle fibers. The efflux curves were kinetically complex and were fit as the sum of three exponentials. In experiments using pretreatment with detergent or $\\sp $C-sucrose the efflux curve lacked the intermediate component. Therefore we concluded that the intermediate component represented movement across a membrane-bound space with a relative fiber volume equivalent to that of the SR. Our estimate of the in situ K$\\sp{+}$ permeability for the SR based on the efflux data is 10$\\sp{-7}$ cm/sec and is sufficient to support a large K$\\sp{+}$ counterion flux.","The effect of a decreased SR K$\\sp{+}$ conductance on the early Ca$\\sp{2+}$ release rate was investigated. An optical technique using the Ca$\\sp{2+}$-sensitive dye Antipyrylazo III was used to monitor caffeine stimulated Ca$\\sp{2+}$ release from skinned skeletal muscle fibers. The SR K$\\sp{+}$ channel blocker bisG10 and substitution of the impermeant ion choline for K$\\sp{+}$ were used as two different methods to decrease SR K$\\sp{+}$ conductance. Both methods caused a concentration dependent decrease in the Ca$\\sp{2+}$ release rate. Therefore we concluded that K$\\sp{+}$ is a counterion for Ca$\\sp{2+}$ during its release from the SR.","The optical technique was then used to determine the selectivity sequence of the in situ SR K$\\sp{+}$ channel to several monovalent cations by substituting them for K$\\sp{+}$. These ions will effect Ca$\\sp{+}$ release dependent on their ability to support counterion flux which is a function of their relative conductance to the SR K$\\sp{+}$ channel. The selectivity sequence determined by these experiments was: K$\\sp{+}$ = Rb$\\sp{+}$ = Na$\\sp{+}$ $>$ Cs$\\sp{+}$ $>$ Li$\\sp{+}$ $>$ choline.","Made available in DSpace on 2014-12-16T06:45:54Z (GMT). No. of bitstreams: 1 8823068.pdf: 3309788 bytes, checksum: d2c644f146b48cf3e19af415350f7132 (MD5) Previous issue date: 1988","Embargo set by: Seth Robbins for item 71621 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","100 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1988."],"dc:identifier":["http://hdl.handle.net/2142/71455","(UMI)AAI8823068"],"dc:subject":["Biology, Animal Physiology"],"dc:title":["The Role of Potassium as a Counterion During Calcium Release From the Sarcoplasmic Reticulum"],"dc:type":["text"],"thesis:degree_discipline":["Physiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:04Z"}