{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/71444"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/71444","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Ionic and Charge Movement Currents in a Sunfish Skeletal Muscle (Membrane Structure, Temperature, E-C Coupling)","abstract":"Electrical properties of striated muscle in a sunfish, Lepomis cyanellus, have been examined using a three microelectrode volt- age clamp method. The preparation was bathed in Ringer solutions designed to suppress unwanted currents and mechanical activity.","abstract_html":"Electrical properties of striated muscle in a sunfish, Lepomis cyanellus, have been examined using a three microelectrode volt- age clamp method. The preparation was bathed in Ringer solutions designed to suppress unwanted currents and mechanical activity.","abstract_has_math":false,"creators":["Klein, Michael Gene"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Physiology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-16T06:45:50Z","date_published":"2014-12-16T06:45:50Z","updated_at":"2026-07-22T22:26:04Z","subjects":["Biology, Animal Physiology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8623343"],"render_values":[{"text":"(UMI)AAI8623343","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/71444","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Klein, Michael Gene"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-16T06:45:50Z","10000-01-01","1986"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Animal Physiology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/71444","(UMI)AAI8623343"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Electrical properties of striated muscle in a sunfish, Lepomis cyanellus, have been examined using a three microelectrode volt- age clamp method. The preparation was bathed in Ringer solutions designed to suppress unwanted currents and mechanical activity.","The ionic currents underlying the action potential consist of a rapidly activating inward current carried by Na('+), a delayed outward current carried by K('+) and a slower outward K('+) current. These cur- rents were fitted to a Hodgkin-Huxley (1952) formalism to quantitate the voltage- and time-dependence of the ionic permeabilities. The Na('+) current was fitted to m('3)h, where the m parameter describes activation and h accounts for spontaneous inactivation. The delayed K('+) current was fitted to n('4) kinetics and the slower K('+) current to s('1) kinetics.","The temperature dependence of activation rate constants for m, n and h were examined in sunfish acclimated to 25 and 7 C. The Arrhenius activation energy of m and h was constant over the range of temperatures examined (0 - 12 C), and was similar in both acclima- tion groups. The rate constant of n,(alpha)(,n), was linear with temperature (0 - 20 C) in cold-adapted sunfish, but exhibited a discontinuity, or "break-point" at 8 C in the relationship of warm adapted fibres such that the K('+) current kinetics were significantly slowed below this tem- perature. The thermal behavior of the K('+) conductance (gK('+)) was identical to the behavior of (alpha)(,n). Various alcohols, alkanes and deter- gents were ineffective in repriming the K('+) current, leaving open the question of a role for membrane lipids in modulating the K('+) current.","Membrane charge movement currents are small capacitative currents which exhibit complicated kinetics in the ON transient, containing apparently two species called Q(,(beta)) and Q(,(gamma)). The steady- state voltage dependence of charge movement (Q - V) is described by a Boltzman distribution with three parameters defining the rela- tionship. In sunfish fibres these are Q(,max) = 29.8 nC uF('-1), V' = -42.9 mV and k = 8.0 mV.","Low temperature paralyzes warm-adapted sunfish fibres. Charge movements in cold-immobilized fibres contain only a single compo- nent of charge. Q(,(beta)) - Q(,(gamma)) is apparently immobilized. The Q - V rela- tionship is described by Q(,max) = 17.9 nC uF('-1), V' = -33.1 mV and k = 16.0 mV. These results support the notion that Q(,(gamma)) is associated with release of Ca('++) from the sarcoplasmic reticulum, and that immobilization of Q(,(gamma)) blocks the Ca('++) release.","Made available in DSpace on 2014-12-16T06:45:50Z (GMT). No. of bitstreams: 1 8623343.pdf: 5142941 bytes, checksum: c1e5d35a7b73d2478af83e1bb8290e42 (MD5) Previous issue date: 1986","Embargo set by: Seth Robbins for item 71610 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","150 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1986."]},{"key":"dc:title","label":"Title","values":["Ionic and Charge Movement Currents in a Sunfish Skeletal Muscle (Membrane Structure, Temperature, E-C Coupling)"]}]}],"canonical_facts":{"dc:creator":["Klein, Michael Gene"],"dc:date":["2014-12-16T06:45:50Z","10000-01-01","1986"],"dc:description":["Electrical properties of striated muscle in a sunfish, Lepomis cyanellus, have been examined using a three microelectrode volt- age clamp method. The preparation was bathed in Ringer solutions designed to suppress unwanted currents and mechanical activity.","The ionic currents underlying the action potential consist of a rapidly activating inward current carried by Na('+), a delayed outward current carried by K('+) and a slower outward K('+) current. These cur- rents were fitted to a Hodgkin-Huxley (1952) formalism to quantitate the voltage- and time-dependence of the ionic permeabilities. The Na('+) current was fitted to m('3)h, where the m parameter describes activation and h accounts for spontaneous inactivation. The delayed K('+) current was fitted to n('4) kinetics and the slower K('+) current to s('1) kinetics.","The temperature dependence of activation rate constants for m, n and h were examined in sunfish acclimated to 25 and 7 C. The Arrhenius activation energy of m and h was constant over the range of temperatures examined (0 - 12 C), and was similar in both acclima- tion groups. The rate constant of n,(alpha)(,n), was linear with temperature (0 - 20 C) in cold-adapted sunfish, but exhibited a discontinuity, or "break-point" at 8 C in the relationship of warm adapted fibres such that the K('+) current kinetics were significantly slowed below this tem- perature. The thermal behavior of the K('+) conductance (gK('+)) was identical to the behavior of (alpha)(,n). Various alcohols, alkanes and deter- gents were ineffective in repriming the K('+) current, leaving open the question of a role for membrane lipids in modulating the K('+) current.","Membrane charge movement currents are small capacitative currents which exhibit complicated kinetics in the ON transient, containing apparently two species called Q(,(beta)) and Q(,(gamma)). The steady- state voltage dependence of charge movement (Q - V) is described by a Boltzman distribution with three parameters defining the rela- tionship. In sunfish fibres these are Q(,max) = 29.8 nC uF('-1), V' = -42.9 mV and k = 8.0 mV.","Low temperature paralyzes warm-adapted sunfish fibres. Charge movements in cold-immobilized fibres contain only a single compo- nent of charge. Q(,(beta)) - Q(,(gamma)) is apparently immobilized. The Q - V rela- tionship is described by Q(,max) = 17.9 nC uF('-1), V' = -33.1 mV and k = 16.0 mV. These results support the notion that Q(,(gamma)) is associated with release of Ca('++) from the sarcoplasmic reticulum, and that immobilization of Q(,(gamma)) blocks the Ca('++) release.","Made available in DSpace on 2014-12-16T06:45:50Z (GMT). No. of bitstreams: 1 8623343.pdf: 5142941 bytes, checksum: c1e5d35a7b73d2478af83e1bb8290e42 (MD5) Previous issue date: 1986","Embargo set by: Seth Robbins for item 71610 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","150 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1986."],"dc:identifier":["http://hdl.handle.net/2142/71444","(UMI)AAI8623343"],"dc:subject":["Biology, Animal Physiology"],"dc:title":["Ionic and Charge Movement Currents in a Sunfish Skeletal Muscle (Membrane Structure, Temperature, E-C Coupling)"],"dc:type":["text"],"thesis:degree_discipline":["Physiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:04Z"}