{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/71340"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/71340","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Applications of in Vitro Cultivation Systems for Plasmodium Falciparum in the Identification and Partial Characterization of Potentially Protective Exoantigens","abstract":"Plasmodium falciparum is the most widespread and pathogenic of four species of parasitic protozoa which cause malaria in humans. Comprehensive studies on the immune response to malaria, and development of effective insecticides, drugs and vaccines are of paramount importance for controlling this dreaded disease.","abstract_html":"Plasmodium falciparum is the most widespread and pathogenic of four species of parasitic protozoa which cause malaria in humans. Comprehensive studies on the immune response to malaria, and development of effective insecticides, drugs and vaccines are of paramount importance for controlling this dreaded disease.","abstract_has_math":false,"creators":["Fajfar Whetstone, Carol Jo T."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Veterinary Medical Science","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-16T06:35:47Z","date_published":"2014-12-16T06:35:47Z","updated_at":"2026-07-22T22:26:04Z","subjects":["Health Sciences, Immunology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8701652"],"render_values":[{"text":"(UMI)AAI8701652","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/71340","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Fajfar Whetstone, Carol Jo T."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-16T06:35:47Z","10000-01-01","1986"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Veterinary Medical Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Health Sciences, Immunology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/71340","(UMI)AAI8701652"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Plasmodium falciparum is the most widespread and pathogenic of four species of parasitic protozoa which cause malaria in humans. Comprehensive studies on the immune response to malaria, and development of effective insecticides, drugs and vaccines are of paramount importance for controlling this dreaded disease.","The asexual blood phase of the parasite life cycle is responsible for the clinical manifestations of malaria, and thus has been a primary focus of vaccine research. It has been shown that parasite antigens (exoantigens) are naturally released into the culture medium as the parasite grows in vitro. Immunization of experimental monkeys with crude or partially purified culture medium can induce protection against acute falciparum malaria.","Parasite exoantigens have been isolated from supernatant fluids of P. falciparum organisms in in vitro systems of human and rabbit/human origin. These antigens are proteins and/or glycoproteins in nature, and are susceptible to heating at 56(DEGREES)C for 30 min. Anion-exchange DEAE-cellulose chromatography has been used to partially purify fractions of supernatants from Indochina I cultures (human and rabbit/human systems) which contain parasite antigens. Crude and partially purified antigens derived from supernatants of Indochina I-infected cultures (human and rabbit/human systems) were immunogenic in mice and rabbits, and were partially protective in a monkey (human system). Target antigens identified in crude and partially purified supernatants of Indochina I-infected cultures include proteins with apparent molecular weights of 79 KD, 40 KD, 29 KD, 20 KD, and 15 KD (human system), and 48 KD, 30 KD, and 27 KD (rabbit/human system). The rabbit/human system of P. falciparum parasites was successively applied in development of a neutralization assay as an in vitro correlate of an antigen's protective potential in vivo. Lastly, the Geneve/SGE-1 strain of P. falciparum was adapted to in vitro growth in a squirrel monkey system as a prerequisite for in vivo adaptation in Bolivian squirrel monkeys. This allowed its use as a challenge strain in experiments designed to assess homologous and heterologous strain immunity.","Made available in DSpace on 2014-12-16T06:35:47Z (GMT). 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Comprehensive studies on the immune response to malaria, and development of effective insecticides, drugs and vaccines are of paramount importance for controlling this dreaded disease.","The asexual blood phase of the parasite life cycle is responsible for the clinical manifestations of malaria, and thus has been a primary focus of vaccine research. It has been shown that parasite antigens (exoantigens) are naturally released into the culture medium as the parasite grows in vitro. Immunization of experimental monkeys with crude or partially purified culture medium can induce protection against acute falciparum malaria.","Parasite exoantigens have been isolated from supernatant fluids of P. falciparum organisms in in vitro systems of human and rabbit/human origin. These antigens are proteins and/or glycoproteins in nature, and are susceptible to heating at 56(DEGREES)C for 30 min. Anion-exchange DEAE-cellulose chromatography has been used to partially purify fractions of supernatants from Indochina I cultures (human and rabbit/human systems) which contain parasite antigens. Crude and partially purified antigens derived from supernatants of Indochina I-infected cultures (human and rabbit/human systems) were immunogenic in mice and rabbits, and were partially protective in a monkey (human system). Target antigens identified in crude and partially purified supernatants of Indochina I-infected cultures include proteins with apparent molecular weights of 79 KD, 40 KD, 29 KD, 20 KD, and 15 KD (human system), and 48 KD, 30 KD, and 27 KD (rabbit/human system). The rabbit/human system of P. falciparum parasites was successively applied in development of a neutralization assay as an in vitro correlate of an antigen's protective potential in vivo. Lastly, the Geneve/SGE-1 strain of P. falciparum was adapted to in vitro growth in a squirrel monkey system as a prerequisite for in vivo adaptation in Bolivian squirrel monkeys. This allowed its use as a challenge strain in experiments designed to assess homologous and heterologous strain immunity.","Made available in DSpace on 2014-12-16T06:35:47Z (GMT). No. of bitstreams: 1 8701652.pdf: 8906111 bytes, checksum: 03f62e8db361b0cd8a62698c9c321c2b (MD5) Previous issue date: 1986","Embargo set by: Seth Robbins for item 71506 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","283 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1986."],"dc:identifier":["http://hdl.handle.net/2142/71340","(UMI)AAI8701652"],"dc:subject":["Health Sciences, Immunology"],"dc:title":["Applications of in Vitro Cultivation Systems for Plasmodium Falciparum in the Identification and Partial Characterization of Potentially Protective Exoantigens"],"dc:type":["text"],"thesis:degree_discipline":["Veterinary Medical Science"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:04Z"}