{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/71339"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/71339","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Influence of Rhodococcus Equi on the Respiratory Burst of Resident Alveolar Macrophages From Horses","abstract":"Rhodococcus equi is the etiologic agent of a devastating pneumonia of sporadic incidence in foals. Opsonized Rhodococcus equi are phagocytosed by equine alveolar macrophages but are ineffectively killed. The respiratory burst of equine neutrophils is obtunded by R. equi. If the bacteria similarly influence equine alveolar macrophages, that could partially explain its facultative intracellular survival.","abstract_html":"Rhodococcus equi is the etiologic agent of a devastating pneumonia of sporadic incidence in foals. Opsonized Rhodococcus equi are phagocytosed by equine alveolar macrophages but are ineffectively killed. The respiratory burst of equine neutrophils is obtunded by R. equi. If the bacteria similarly influence equine alveolar macrophages, that could partially explain its facultative intracellular survival.","abstract_has_math":false,"creators":["Brumbaugh, Gordon Wayne"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Veterinary Medical Science","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-16T06:35:46Z","date_published":"2014-12-16T06:35:46Z","updated_at":"2026-07-22T22:26:04Z","subjects":["Biology, Veterinary Science","Health Sciences, Immunology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8701443"],"render_values":[{"text":"(UMI)AAI8701443","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/71339","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Brumbaugh, Gordon Wayne"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-16T06:35:46Z","10000-01-01","1986"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Veterinary Medical Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Veterinary Science","Health Sciences, Immunology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/71339","(UMI)AAI8701443"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Rhodococcus equi is the etiologic agent of a devastating pneumonia of sporadic incidence in foals. Opsonized Rhodococcus equi are phagocytosed by equine alveolar macrophages but are ineffectively killed. The respiratory burst of equine neutrophils is obtunded by R. equi. If the bacteria similarly influence equine alveolar macrophages, that could partially explain its facultative intracellular survival.","The purpose of this study was to evaluate the influence of R. equi on the superoxide anion production, measured spectrophotometrically as the reduction of cytochrome C, and hexose monophosphate shunt activity, measured by ('14)CO(,2) liberation from ('14)C-1-D-glucose, of alveolar macrophages from horses. Alveolar macrophages were harvested from 6 anesthetized, healthy, light-breed, adult horses by bronchoalveolar lavage. Following a randomized complete block design, the suspension of cells was divided into aliquots of 10('6) viable alveolar macrophages which were exposed to 1, 10, or 100 g. of opsonized R. equi or opsonized zymosan A at 37 C for 2 hours.","The amount of superoxide anion produced by alveolar macrophages stimulated with three masses each of opsonized zymosan A and opsonized R. equi was not significantly different from that produced by unstimulated cells. The hexose monophosphate shunt activity was enhanced by both stimulants in a mass-related manner. No significant difference was found in the response produced by equal masses of either stimulant.","In this study the respiratory burst of equine alveolar macrophages was only evidenced by the hexose monophosphate shunt activity and superoxide anion was not coincidently produced. Rhodococcus equi did not adversely affect that response. The insignificant superoxide anion production by the alveolar macrophages suggests that this may not be a significant oxygen metabolite in those cells.","Further studies directed at defining the production of superoxide anion by equine alveolar macrophages should be performed. Studies of bacterial interaction with alveolar macrophages from foals should also be performed.","Made available in DSpace on 2014-12-16T06:35:46Z (GMT). No. of bitstreams: 1 8701443.pdf: 5755768 bytes, checksum: f3653979631e7c9ffb63a838dfa3d349 (MD5) Previous issue date: 1986","Embargo set by: Seth Robbins for item 71505 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","161 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1986."]},{"key":"dc:title","label":"Title","values":["Influence of Rhodococcus Equi on the Respiratory Burst of Resident Alveolar Macrophages From Horses"]}]}],"canonical_facts":{"dc:creator":["Brumbaugh, Gordon Wayne"],"dc:date":["2014-12-16T06:35:46Z","10000-01-01","1986"],"dc:description":["Rhodococcus equi is the etiologic agent of a devastating pneumonia of sporadic incidence in foals. Opsonized Rhodococcus equi are phagocytosed by equine alveolar macrophages but are ineffectively killed. The respiratory burst of equine neutrophils is obtunded by R. equi. If the bacteria similarly influence equine alveolar macrophages, that could partially explain its facultative intracellular survival.","The purpose of this study was to evaluate the influence of R. equi on the superoxide anion production, measured spectrophotometrically as the reduction of cytochrome C, and hexose monophosphate shunt activity, measured by ('14)CO(,2) liberation from ('14)C-1-D-glucose, of alveolar macrophages from horses. Alveolar macrophages were harvested from 6 anesthetized, healthy, light-breed, adult horses by bronchoalveolar lavage. Following a randomized complete block design, the suspension of cells was divided into aliquots of 10('6) viable alveolar macrophages which were exposed to 1, 10, or 100 g. of opsonized R. equi or opsonized zymosan A at 37 C for 2 hours.","The amount of superoxide anion produced by alveolar macrophages stimulated with three masses each of opsonized zymosan A and opsonized R. equi was not significantly different from that produced by unstimulated cells. The hexose monophosphate shunt activity was enhanced by both stimulants in a mass-related manner. No significant difference was found in the response produced by equal masses of either stimulant.","In this study the respiratory burst of equine alveolar macrophages was only evidenced by the hexose monophosphate shunt activity and superoxide anion was not coincidently produced. Rhodococcus equi did not adversely affect that response. The insignificant superoxide anion production by the alveolar macrophages suggests that this may not be a significant oxygen metabolite in those cells.","Further studies directed at defining the production of superoxide anion by equine alveolar macrophages should be performed. Studies of bacterial interaction with alveolar macrophages from foals should also be performed.","Made available in DSpace on 2014-12-16T06:35:46Z (GMT). No. of bitstreams: 1 8701443.pdf: 5755768 bytes, checksum: f3653979631e7c9ffb63a838dfa3d349 (MD5) Previous issue date: 1986","Embargo set by: Seth Robbins for item 71505 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","161 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1986."],"dc:identifier":["http://hdl.handle.net/2142/71339","(UMI)AAI8701443"],"dc:subject":["Biology, Veterinary Science","Health Sciences, Immunology"],"dc:title":["Influence of Rhodococcus Equi on the Respiratory Burst of Resident Alveolar Macrophages From Horses"],"dc:type":["text"],"thesis:degree_discipline":["Veterinary Medical Science"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:04Z"}