{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/71315"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/71315","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Inflammation, Phagocytosis and Effect of Flunixin Meglumine During Endotoxin-Induced Mastitis (Therapy, Prostaglandins)","abstract":"The objectives were to determine the following in endotoxin-induced mastitis: (1) milk concentrations of thromboxane B(,2) (TXB(,2)) and prostaglandin F(,2(alpha)) (PGF(,2(alpha))); (2) the pharmacokinetics of flunixin meglumine in lactating cattle; (3) the clinical efficacy of the flunixin meglumine and its effect on milk and plasma TXB(,2) and PGF(,2(alpha)); and (4) changes of whey immunoglobulins and ability of wheys to enhance neutrophil phagocytosis of Staphylococcus aureus.","abstract_html":"The objectives were to determine the following in endotoxin-induced mastitis: (1) milk concentrations of thromboxane B(,2) (TXB(,2)) and prostaglandin F(,2(alpha)) (PGF(,2(alpha))); (2) the pharmacokinetics of flunixin meglumine in lactating cattle; (3) the clinical efficacy of the flunixin meglumine and its effect on milk and plasma TXB(,2) and PGF(,2(alpha)); and (4) changes of whey immunoglobulins and ability of wheys to enhance neutrophil phagocytosis of Staphylococcus aureus.","abstract_has_math":false,"creators":["Anderson, Kevin Lindsay"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Veterinary Medical Science","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-16T06:35:35Z","date_published":"2014-12-16T06:35:35Z","updated_at":"2026-07-22T22:26:04Z","subjects":["Biology, Veterinary Science"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8502061"],"render_values":[{"text":"(UMI)AAI8502061","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/71315","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Anderson, Kevin Lindsay"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-16T06:35:35Z","10000-01-01","1984"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Veterinary Medical Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Veterinary Science"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/71315","(UMI)AAI8502061"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The objectives were to determine the following in endotoxin-induced mastitis: (1) milk concentrations of thromboxane B(,2) (TXB(,2)) and prostaglandin F(,2(alpha)) (PGF(,2(alpha))); (2) the pharmacokinetics of flunixin meglumine in lactating cattle; (3) the clinical efficacy of the flunixin meglumine and its effect on milk and plasma TXB(,2) and PGF(,2(alpha)); and (4) changes of whey immunoglobulins and ability of wheys to enhance neutrophil phagocytosis of Staphylococcus aureus.","Initial studies were conducted on clinical cases of coliform mastitis and showed increased TXB(,2) and PGF(,2(alpha)) in quarter milk samples. In studies in 5 cows inoculated (in single quarters) with Escherichia coli, milk bovine serum albumin and somatic cell concentrations increased significantly in inoculated quarters. Concentrations of TXB(,2) and PGF(,2(alpha)) were not significantly elevated in inoculated quarters. In further studies, 10 (mu)g of endotoxin (E. coli 026:(beta)6 lipopolysaccharide) was inoculated in single quarters of 12 cows. Concentrations of TXB(,2) in milk were elevated in endotoxin-treated quarters. Concentrations of PGF(,2(alpha)) in milk were not significantly different among quarter treatments.","Concentrations of flunixin were determined in plasma and milk of 6 cows following administration of 1.1 mg/kg doses of flunixin meglumine in a two-way cross-over experiment. The pharmacokinetics of flunixin were best described by a two-compartment open model with a mean plasma half-life (t- 1/2 (beta)) of 3.6 hours. A dosing schedule of 1.1 mg/kg at 8-hour intervals was indicated.","Flunixin treatment IM (6 cows) was compared to saline IM (6 cows) in 12 cows administered 10 (mu)g of endotoxin in single quarters. Flunixin reduced rectal temperatures, depression scores, and quarter inflammation as well as 15-keto-13,14-dihydro PGF(,2(alpha)) in jugular vein plasma and TXB(,2) in milk. Milk indicators of acute mastitis and milk production were not significantly different between IM treatments.","Whey immunoglobulin concentrations and the ability of wheys to enhance phagocytosis of Staphylococcus aureus by neutrophils were determined in samples of 12 cows following inoculation of endotoxin. IgG(,1), IgG(,2) and IgM and ability of wheys to enhance phagocytosis increased in endotoxin-treated quarters. Immunoglobulins (primarily IgG(,2)), divalent cations and complement all contributed to the increased phagocytosis.","Made available in DSpace on 2014-12-16T06:35:35Z (GMT). No. of bitstreams: 1 8502061.pdf: 9731012 bytes, checksum: e24fcd065c0ff1622920c81b88b48385 (MD5) Previous issue date: 1984","Embargo set by: Seth Robbins for item 71481 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","324 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1984."]},{"key":"dc:title","label":"Title","values":["Inflammation, Phagocytosis and Effect of Flunixin Meglumine During Endotoxin-Induced Mastitis (Therapy, Prostaglandins)"]}]}],"canonical_facts":{"dc:creator":["Anderson, Kevin Lindsay"],"dc:date":["2014-12-16T06:35:35Z","10000-01-01","1984"],"dc:description":["The objectives were to determine the following in endotoxin-induced mastitis: (1) milk concentrations of thromboxane B(,2) (TXB(,2)) and prostaglandin F(,2(alpha)) (PGF(,2(alpha))); (2) the pharmacokinetics of flunixin meglumine in lactating cattle; (3) the clinical efficacy of the flunixin meglumine and its effect on milk and plasma TXB(,2) and PGF(,2(alpha)); and (4) changes of whey immunoglobulins and ability of wheys to enhance neutrophil phagocytosis of Staphylococcus aureus.","Initial studies were conducted on clinical cases of coliform mastitis and showed increased TXB(,2) and PGF(,2(alpha)) in quarter milk samples. In studies in 5 cows inoculated (in single quarters) with Escherichia coli, milk bovine serum albumin and somatic cell concentrations increased significantly in inoculated quarters. Concentrations of TXB(,2) and PGF(,2(alpha)) were not significantly elevated in inoculated quarters. In further studies, 10 (mu)g of endotoxin (E. coli 026:(beta)6 lipopolysaccharide) was inoculated in single quarters of 12 cows. Concentrations of TXB(,2) in milk were elevated in endotoxin-treated quarters. Concentrations of PGF(,2(alpha)) in milk were not significantly different among quarter treatments.","Concentrations of flunixin were determined in plasma and milk of 6 cows following administration of 1.1 mg/kg doses of flunixin meglumine in a two-way cross-over experiment. The pharmacokinetics of flunixin were best described by a two-compartment open model with a mean plasma half-life (t- 1/2 (beta)) of 3.6 hours. A dosing schedule of 1.1 mg/kg at 8-hour intervals was indicated.","Flunixin treatment IM (6 cows) was compared to saline IM (6 cows) in 12 cows administered 10 (mu)g of endotoxin in single quarters. Flunixin reduced rectal temperatures, depression scores, and quarter inflammation as well as 15-keto-13,14-dihydro PGF(,2(alpha)) in jugular vein plasma and TXB(,2) in milk. Milk indicators of acute mastitis and milk production were not significantly different between IM treatments.","Whey immunoglobulin concentrations and the ability of wheys to enhance phagocytosis of Staphylococcus aureus by neutrophils were determined in samples of 12 cows following inoculation of endotoxin. IgG(,1), IgG(,2) and IgM and ability of wheys to enhance phagocytosis increased in endotoxin-treated quarters. Immunoglobulins (primarily IgG(,2)), divalent cations and complement all contributed to the increased phagocytosis.","Made available in DSpace on 2014-12-16T06:35:35Z (GMT). No. of bitstreams: 1 8502061.pdf: 9731012 bytes, checksum: e24fcd065c0ff1622920c81b88b48385 (MD5) Previous issue date: 1984","Embargo set by: Seth Robbins for item 71481 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","324 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1984."],"dc:identifier":["http://hdl.handle.net/2142/71315","(UMI)AAI8502061"],"dc:subject":["Biology, Veterinary Science"],"dc:title":["Inflammation, Phagocytosis and Effect of Flunixin Meglumine During Endotoxin-Induced Mastitis (Therapy, Prostaglandins)"],"dc:type":["text"],"thesis:degree_discipline":["Veterinary Medical Science"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:04Z"}