{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/71295"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/71295","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Studies of Culture-Derived Soluble Babesia Bovis Antigens: Purification, Characterization and Application in Serodiagnosis","abstract":"Analytical purification of soluble antigens from crude supernatant fluids of Babesia bovis cultures was conducted by various immunoadsorption procedures using horse anti-B. bovis IgG. A Protein A-Sepharose CL-4B adsorbent proved the most efficient as 58 mg of antigen could be purified from 34 ml of culture fluid. Isolated antigens were identified by crossed immunoelectrophoresis.","abstract_html":"Analytical purification of soluble antigens from crude supernatant fluids of Babesia bovis cultures was conducted by various immunoadsorption procedures using horse anti-B. bovis IgG. A Protein A-Sepharose CL-4B adsorbent proved the most efficient as 58 mg of antigen could be purified from 34 ml of culture fluid. Isolated antigens were identified by crossed immunoelectrophoresis.","abstract_has_math":false,"creators":["Montenegro Heredia, Sonia Armonia"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Veterinary Medical Science","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-16T06:35:23Z","date_published":"2014-12-16T06:35:23Z","updated_at":"2026-07-22T22:26:04Z","subjects":["Biology, Veterinary Science"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8218525"],"render_values":[{"text":"(UMI)AAI8218525","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/71295","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Montenegro Heredia, Sonia Armonia"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-16T06:35:23Z","10000-01-01","1982"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Veterinary Medical Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Veterinary Science"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/71295","(UMI)AAI8218525"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Analytical purification of soluble antigens from crude supernatant fluids of Babesia bovis cultures was conducted by various immunoadsorption procedures using horse anti-B. bovis IgG. A Protein A-Sepharose CL-4B adsorbent proved the most efficient as 58 mg of antigen could be purified from 34 ml of culture fluid. Isolated antigens were identified by crossed immunoelectrophoresis.","Subsequent preparative purification of three soluble babesial antigens was performed with ammonium sulfate precipitation and anion exchange chromatography. The antigens were precipitated with 60-70% saturated ammonium sulfate. Also, a 0.1M Tris-HCl buffer pH 6.5 selectively eluted the Babesia antigens from DEAE-cellulose with minimal serum contamination.","Monospecific rabbit antibodies produced against individual B. bovis antigens were used in an immunofluorescence test to identify the location of the respective antigens within the infected erythrocyte. Two antigens were found on or near the erythrocyte membrane, while a third antigen was associated directly with the parasite itself.","Additionally, the development of two assay techniques was an integral part of this study. The protein A bacterial adsorbent co-agglutination test was conceived for the quantitation of soluble antigens found in culture supernatant fluids. These antigens were also utilized in the latex agglutination test, a simple and practical serodiagnostic procedure developed for the detection of bovine babesiosis.","Made available in DSpace on 2014-12-16T06:35:23Z (GMT). No. of bitstreams: 1 8218525.pdf: 3675556 bytes, checksum: 9049437b1f657236bdf361a561eae099 (MD5) Previous issue date: 1982","Embargo set by: Seth Robbins for item 71461 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","113 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1982."]},{"key":"dc:title","label":"Title","values":["Studies of Culture-Derived Soluble Babesia Bovis Antigens: Purification, Characterization and Application in Serodiagnosis"]}]}],"canonical_facts":{"dc:creator":["Montenegro Heredia, Sonia Armonia"],"dc:date":["2014-12-16T06:35:23Z","10000-01-01","1982"],"dc:description":["Analytical purification of soluble antigens from crude supernatant fluids of Babesia bovis cultures was conducted by various immunoadsorption procedures using horse anti-B. bovis IgG. A Protein A-Sepharose CL-4B adsorbent proved the most efficient as 58 mg of antigen could be purified from 34 ml of culture fluid. Isolated antigens were identified by crossed immunoelectrophoresis.","Subsequent preparative purification of three soluble babesial antigens was performed with ammonium sulfate precipitation and anion exchange chromatography. The antigens were precipitated with 60-70% saturated ammonium sulfate. Also, a 0.1M Tris-HCl buffer pH 6.5 selectively eluted the Babesia antigens from DEAE-cellulose with minimal serum contamination.","Monospecific rabbit antibodies produced against individual B. bovis antigens were used in an immunofluorescence test to identify the location of the respective antigens within the infected erythrocyte. Two antigens were found on or near the erythrocyte membrane, while a third antigen was associated directly with the parasite itself.","Additionally, the development of two assay techniques was an integral part of this study. The protein A bacterial adsorbent co-agglutination test was conceived for the quantitation of soluble antigens found in culture supernatant fluids. These antigens were also utilized in the latex agglutination test, a simple and practical serodiagnostic procedure developed for the detection of bovine babesiosis.","Made available in DSpace on 2014-12-16T06:35:23Z (GMT). No. of bitstreams: 1 8218525.pdf: 3675556 bytes, checksum: 9049437b1f657236bdf361a561eae099 (MD5) Previous issue date: 1982","Embargo set by: Seth Robbins for item 71461 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","113 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1982."],"dc:identifier":["http://hdl.handle.net/2142/71295","(UMI)AAI8218525"],"dc:subject":["Biology, Veterinary Science"],"dc:title":["Studies of Culture-Derived Soluble Babesia Bovis Antigens: Purification, Characterization and Application in Serodiagnosis"],"dc:type":["text"],"thesis:degree_discipline":["Veterinary Medical Science"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:04Z"}