{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/71287"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/71287","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"The Effects of Chemical Modification Upon Herbicide Binding in Thylakoid Membranes of Spinacia Oleracea (Spinach) and Amaranthus Hybridus (Pigweed), and The Photochemical Reaction Center of Rhodobacter Sphaeroides R26","abstract":"Dicyclohexylcarbodiimide inhibits electron transfer between Q$\\sb{\\rm A}$ and Q$\\sb{\\rm B}$ in both higher plants and photosynthetic bacteria in an analogous manner to that seen for the classic Photosystem II inhibitors such as diuron and atrazine. It does not bind to the same binding domain as these inhibitors. It does not bind covalently, suggesting that the mechanism of inhibition in this system is a novel one for this protein modification reagent.","abstract_html":"Dicyclohexylcarbodiimide inhibits electron transfer between Q$\\sb{\\rm A}$ and Q$\\sb{\\rm B}$ in both higher plants and photosynthetic bacteria in an analogous manner to that seen for the classic Photosystem II inhibitors such as diuron and atrazine. It does not bind to the same binding domain as these inhibitors. It does not bind covalently, suggesting that the mechanism of inhibition in this system is a novel one for this protein modification reagent.","abstract_has_math":true,"creators":["Paterson, David Ross"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Plant Biology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-16T06:21:59Z","date_published":"2014-12-16T06:21:59Z","updated_at":"2026-07-22T22:26:04Z","subjects":["Agriculture, Plant Culture"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8721732"],"render_values":[{"text":"(UMI)AAI8721732","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/71287","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Paterson, David Ross"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-16T06:21:59Z","10000-01-01","1987"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Plant Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Plant Culture"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/71287","(UMI)AAI8721732"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Dicyclohexylcarbodiimide inhibits electron transfer between Q$\\sb{\\rm A}$ and Q$\\sb{\\rm B}$ in both higher plants and photosynthetic bacteria in an analogous manner to that seen for the classic Photosystem II inhibitors such as diuron and atrazine. It does not bind to the same binding domain as these inhibitors. It does not bind covalently, suggesting that the mechanism of inhibition in this system is a novel one for this protein modification reagent.","Amaranthus hybridus shows a heterogeneous population of diuron binding sites. Two populations are readily observed in the absence of ferricyanide. This heterogeneity of binding sites is also seen in spinach, but in the presence of ferricyanide.","The structural integrity of Photosystem II plays a crucial role in diuron binding. When the membrane structure is altered by detergent treatment, removal of divalent cations, or phosphorylation, the binding affinity of diuron is increased.","Azido-diuron binds to two polypeptides in thylakoid membranes. A 32 kD polypeptide which is probably the so-called herbicide binding protein. The other polypeptide has an apparent molecular weight of 50 kD. The identity of the polypeptide is unknown.","Models for predicting secondary structure were used to examine the herbicide binding domain, and found to be unsuitable for predicting secondary structure in integral membrane proteins.","Made available in DSpace on 2014-12-16T06:21:59Z (GMT). No. of bitstreams: 1 8721732.pdf: 7286637 bytes, checksum: 1b43f1e3768e23a7b5a924c01057a5f5 (MD5) Previous issue date: 1987","Embargo set by: Seth Robbins for item 71453 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","282 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1987."]},{"key":"dc:title","label":"Title","values":["The Effects of Chemical Modification Upon Herbicide Binding in Thylakoid Membranes of Spinacia Oleracea (Spinach) and Amaranthus Hybridus (Pigweed), and The Photochemical Reaction Center of Rhodobacter Sphaeroides R26"]}]}],"canonical_facts":{"dc:creator":["Paterson, David Ross"],"dc:date":["2014-12-16T06:21:59Z","10000-01-01","1987"],"dc:description":["Dicyclohexylcarbodiimide inhibits electron transfer between Q$\\sb{\\rm A}$ and Q$\\sb{\\rm B}$ in both higher plants and photosynthetic bacteria in an analogous manner to that seen for the classic Photosystem II inhibitors such as diuron and atrazine. It does not bind to the same binding domain as these inhibitors. It does not bind covalently, suggesting that the mechanism of inhibition in this system is a novel one for this protein modification reagent.","Amaranthus hybridus shows a heterogeneous population of diuron binding sites. Two populations are readily observed in the absence of ferricyanide. This heterogeneity of binding sites is also seen in spinach, but in the presence of ferricyanide.","The structural integrity of Photosystem II plays a crucial role in diuron binding. When the membrane structure is altered by detergent treatment, removal of divalent cations, or phosphorylation, the binding affinity of diuron is increased.","Azido-diuron binds to two polypeptides in thylakoid membranes. A 32 kD polypeptide which is probably the so-called herbicide binding protein. The other polypeptide has an apparent molecular weight of 50 kD. The identity of the polypeptide is unknown.","Models for predicting secondary structure were used to examine the herbicide binding domain, and found to be unsuitable for predicting secondary structure in integral membrane proteins.","Made available in DSpace on 2014-12-16T06:21:59Z (GMT). No. of bitstreams: 1 8721732.pdf: 7286637 bytes, checksum: 1b43f1e3768e23a7b5a924c01057a5f5 (MD5) Previous issue date: 1987","Embargo set by: Seth Robbins for item 71453 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","282 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1987."],"dc:identifier":["http://hdl.handle.net/2142/71287","(UMI)AAI8721732"],"dc:subject":["Agriculture, Plant Culture"],"dc:title":["The Effects of Chemical Modification Upon Herbicide Binding in Thylakoid Membranes of Spinacia Oleracea (Spinach) and Amaranthus Hybridus (Pigweed), and The Photochemical Reaction Center of Rhodobacter Sphaeroides R26"],"dc:type":["text"],"thesis:degree_discipline":["Plant Biology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:04Z"}