{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/71179"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/71179","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Characterization, Expression and Genetic Analysis of Recurrent Idiotypes in the Unrestricted Anti-Fluorescein Immune Response","abstract":"Eighteen monoclonal anti-fluorescein (Fl) antibodies from BALB/cV mice were characterized and 13 possessed a low affinity for Fl (K(,a) = 5 x 10('4) M('-1)) while 5 had a higher affinity (K(,a) (TBOND) 5 x 10('8) M('-1)). Eleven of the 13 low affinity clones were idiotypically cross-reactive (40-100%) and 4 of these clones possessed identical idiotypes (Ids) (3-12, 3-13, 3-17 and 3-35). Heavy and light chains derived from clones 3-12, 3-13 and 3-17 and used in recombination experiments, resulted in the reformation of an anti-Fl active site in all six heterologous combinations. The expression of Ids 3-13 and 3-17 in the I(DEGREES), II(DEGREES) or III(DEGREES) polyclonal anti-Fl response was quantitated and found to represent (LESSTHEQ) 1.0% of the specific repertoire. Ids 3-13 and 3-17 were expressed in 15/22 ad 22/28 individual mice, respectively. Clones 3-13 and 3-17 had similar though not identical active sites as determined by fine specificity of binding and Id inhibitions. Ids 3-13 and 3-17 represent the first demonstration of repertoire-specific recurrent Ids expressed during a T-dependent immune response lacking a dominant Id. Antibodies 3-13 and 3-17 were idiotypically cross-reactive (10-40%) with 6 other anti-Fl clones (designated the 9-40 family) which possessed a higher affinity for Fl (K(,a) (TURN) 5 x 10('6) M('-1)). Members of the 9-40 family were also idiotypically cross-reactive with a single high affinity clone 4-4 (K(,a) = 5 x 10('9) M('-1)). This idiotypic linkage provided a greater span in affinity (>20,000 fold) than in all previously characterized Id families within restricted or unrestricted immune responses. The sequence of antibody 3-13 V(,H) region was determined by genomic cloning and amino acid sequencing and was 78% homologous to the (alpha)-1,3-dextran binding myeloma proteins J558 and 104E. Rearrangement of a V(,H) gene homologous to 3-13 was required idiotypically cross-reactive clones and in no idiotypically distinct clones.","abstract_html":"Eighteen monoclonal anti-fluorescein (Fl) antibodies from BALB/cV mice were characterized and 13 possessed a low affinity for Fl (K(,a) = 5 x 10('4) M('-1)) while 5 had a higher affinity (K(,a) (TBOND) 5 x 10('8) M('-1)). Eleven of the 13 low affinity clones were idiotypically cross-reactive (40-100%) and 4 of these clones possessed identical idiotypes (Ids) (3-12, 3-13, 3-17 and 3-35). Heavy and light chains derived from clones 3-12, 3-13 and 3-17 and used in recombination experiments, resulted in the reformation of an anti-Fl active site in all six heterologous combinations. The expression of Ids 3-13 and 3-17 in the I(DEGREES), II(DEGREES) or III(DEGREES) polyclonal anti-Fl response was quantitated and found to represent (LESSTHEQ) 1.0% of the specific repertoire. Ids 3-13 and 3-17 were expressed in 15/22 ad 22/28 individual mice, respectively. Clones 3-13 and 3-17 had similar though not identical active sites as determined by fine specificity of binding and Id inhibitions. Ids 3-13 and 3-17 represent the first demonstration of repertoire-specific recurrent Ids expressed during a T-dependent immune response lacking a dominant Id. Antibodies 3-13 and 3-17 were idiotypically cross-reactive (10-40%) with 6 other anti-Fl clones (designated the 9-40 family) which possessed a higher affinity for Fl (K(,a) (TURN) 5 x 10('6) M('-1)). Members of the 9-40 family were also idiotypically cross-reactive with a single high affinity clone 4-4 (K(,a) = 5 x 10('9) M('-1)). This idiotypic linkage provided a greater span in affinity (>20,000 fold) than in all previously characterized Id families within restricted or unrestricted immune responses. The sequence of antibody 3-13 V(,H) region was determined by genomic cloning and amino acid sequencing and was 78% homologous to the (alpha)-1,3-dextran binding myeloma proteins J558 and 104E. Rearrangement of a V(,H) gene homologous to 3-13 was required idiotypically cross-reactive clones and in no idiotypically distinct clones.","abstract_has_math":false,"creators":["Reinitz, David Maximilian"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-16T06:12:58Z","date_published":"2014-12-16T06:12:58Z","updated_at":"2026-07-22T22:26:04Z","subjects":["Biology, Microbiology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8701597"],"render_values":[{"text":"(UMI)AAI8701597","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/71179","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Reinitz, David Maximilian"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-16T06:12:58Z","10000-01-01","1986"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Microbiology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/71179","(UMI)AAI8701597"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Eighteen monoclonal anti-fluorescein (Fl) antibodies from BALB/cV mice were characterized and 13 possessed a low affinity for Fl (K(,a) = 5 x 10('4) M('-1)) while 5 had a higher affinity (K(,a) (TBOND) 5 x 10('8) M('-1)). Eleven of the 13 low affinity clones were idiotypically cross-reactive (40-100%) and 4 of these clones possessed identical idiotypes (Ids) (3-12, 3-13, 3-17 and 3-35). Heavy and light chains derived from clones 3-12, 3-13 and 3-17 and used in recombination experiments, resulted in the reformation of an anti-Fl active site in all six heterologous combinations. The expression of Ids 3-13 and 3-17 in the I(DEGREES), II(DEGREES) or III(DEGREES) polyclonal anti-Fl response was quantitated and found to represent (LESSTHEQ) 1.0% of the specific repertoire. Ids 3-13 and 3-17 were expressed in 15/22 ad 22/28 individual mice, respectively. Clones 3-13 and 3-17 had similar though not identical active sites as determined by fine specificity of binding and Id inhibitions. Ids 3-13 and 3-17 represent the first demonstration of repertoire-specific recurrent Ids expressed during a T-dependent immune response lacking a dominant Id. Antibodies 3-13 and 3-17 were idiotypically cross-reactive (10-40%) with 6 other anti-Fl clones (designated the 9-40 family) which possessed a higher affinity for Fl (K(,a) (TURN) 5 x 10('6) M('-1)). Members of the 9-40 family were also idiotypically cross-reactive with a single high affinity clone 4-4 (K(,a) = 5 x 10('9) M('-1)). This idiotypic linkage provided a greater span in affinity (>20,000 fold) than in all previously characterized Id families within restricted or unrestricted immune responses. The sequence of antibody 3-13 V(,H) region was determined by genomic cloning and amino acid sequencing and was 78% homologous to the (alpha)-1,3-dextran binding myeloma proteins J558 and 104E. Rearrangement of a V(,H) gene homologous to 3-13 was required idiotypically cross-reactive clones and in no idiotypically distinct clones.","Made available in DSpace on 2014-12-16T06:12:58Z (GMT). No. of bitstreams: 1 8701597.pdf: 6769684 bytes, checksum: a36e1e50040530423f7d0429a23d6f6e (MD5) Previous issue date: 1986","Embargo set by: Seth Robbins for item 71345 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","217 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1986."]},{"key":"dc:title","label":"Title","values":["Characterization, Expression and Genetic Analysis of Recurrent Idiotypes in the Unrestricted Anti-Fluorescein Immune Response"]}]}],"canonical_facts":{"dc:creator":["Reinitz, David Maximilian"],"dc:date":["2014-12-16T06:12:58Z","10000-01-01","1986"],"dc:description":["Eighteen monoclonal anti-fluorescein (Fl) antibodies from BALB/cV mice were characterized and 13 possessed a low affinity for Fl (K(,a) = 5 x 10('4) M('-1)) while 5 had a higher affinity (K(,a) (TBOND) 5 x 10('8) M('-1)). Eleven of the 13 low affinity clones were idiotypically cross-reactive (40-100%) and 4 of these clones possessed identical idiotypes (Ids) (3-12, 3-13, 3-17 and 3-35). Heavy and light chains derived from clones 3-12, 3-13 and 3-17 and used in recombination experiments, resulted in the reformation of an anti-Fl active site in all six heterologous combinations. The expression of Ids 3-13 and 3-17 in the I(DEGREES), II(DEGREES) or III(DEGREES) polyclonal anti-Fl response was quantitated and found to represent (LESSTHEQ) 1.0% of the specific repertoire. Ids 3-13 and 3-17 were expressed in 15/22 ad 22/28 individual mice, respectively. Clones 3-13 and 3-17 had similar though not identical active sites as determined by fine specificity of binding and Id inhibitions. Ids 3-13 and 3-17 represent the first demonstration of repertoire-specific recurrent Ids expressed during a T-dependent immune response lacking a dominant Id. Antibodies 3-13 and 3-17 were idiotypically cross-reactive (10-40%) with 6 other anti-Fl clones (designated the 9-40 family) which possessed a higher affinity for Fl (K(,a) (TURN) 5 x 10('6) M('-1)). Members of the 9-40 family were also idiotypically cross-reactive with a single high affinity clone 4-4 (K(,a) = 5 x 10('9) M('-1)). This idiotypic linkage provided a greater span in affinity (>20,000 fold) than in all previously characterized Id families within restricted or unrestricted immune responses. The sequence of antibody 3-13 V(,H) region was determined by genomic cloning and amino acid sequencing and was 78% homologous to the (alpha)-1,3-dextran binding myeloma proteins J558 and 104E. Rearrangement of a V(,H) gene homologous to 3-13 was required idiotypically cross-reactive clones and in no idiotypically distinct clones.","Made available in DSpace on 2014-12-16T06:12:58Z (GMT). No. of bitstreams: 1 8701597.pdf: 6769684 bytes, checksum: a36e1e50040530423f7d0429a23d6f6e (MD5) Previous issue date: 1986","Embargo set by: Seth Robbins for item 71345 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","217 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1986."],"dc:identifier":["http://hdl.handle.net/2142/71179","(UMI)AAI8701597"],"dc:subject":["Biology, Microbiology"],"dc:title":["Characterization, Expression and Genetic Analysis of Recurrent Idiotypes in the Unrestricted Anti-Fluorescein Immune Response"],"dc:type":["text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:04Z"}