{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/71169"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/71169","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Monoclonal Analysis of Spontaneous and Induced Antibody Responses in Autoimmune New Zealand Mice","abstract":"New Zealand BW mice spontaneously develop lymphocytic disorders and express autoantibodies in association with an autoimmune disease similar to human SLE. A comparative monoclonal analysis of spontaneous and experimentally-induced antibody responses in autoimmune BW mice was facilitated by hybridoma technology. Various immunochemical and physicochemical parameters of generated monoclonal antibodies were examined to provide a qualitative functional and structural description of such etiologically distinct antibody repertoires in the context of inherent cellular and humoral abnormalities characteristic of this strain.","abstract_html":"New Zealand BW mice spontaneously develop lymphocytic disorders and express autoantibodies in association with an autoimmune disease similar to human SLE. A comparative monoclonal analysis of spontaneous and experimentally-induced antibody responses in autoimmune BW mice was facilitated by hybridoma technology. Various immunochemical and physicochemical parameters of generated monoclonal antibodies were examined to provide a qualitative functional and structural description of such etiologically distinct antibody repertoires in the context of inherent cellular and humoral abnormalities characteristic of this strain.","abstract_has_math":false,"creators":["Ballard, Dean Williams"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-16T06:12:54Z","date_published":"2014-12-16T06:12:54Z","updated_at":"2026-07-22T22:26:04Z","subjects":["Health Sciences, Immunology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8511577"],"render_values":[{"text":"(UMI)AAI8511577","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/71169","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Ballard, Dean Williams"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-16T06:12:54Z","10000-01-01","1985"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Health Sciences, Immunology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/71169","(UMI)AAI8511577"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["New Zealand BW mice spontaneously develop lymphocytic disorders and express autoantibodies in association with an autoimmune disease similar to human SLE. A comparative monoclonal analysis of spontaneous and experimentally-induced antibody responses in autoimmune BW mice was facilitated by hybridoma technology. Various immunochemical and physicochemical parameters of generated monoclonal antibodies were examined to provide a qualitative functional and structural description of such etiologically distinct antibody repertoires in the context of inherent cellular and humoral abnormalities characteristic of this strain.","Ten monoclonal anti-DNA autoantibodies, derived from nonmanipulated BW mice and specific for various forms of DNA, were found to contain either IgG2a or IgG2b heavy chains related by a cross-reactive allotypic determinant. Binding studies, which employed defined DNA restriction fragments and synthetic nucleic acids, indicated that: (1) both helical conformation and nucleotide composition were involved in antibody recognition of duplex and single-stranded DNA, and (2) anti-DNA antibodies formed stable complexes with short synthetic oligonucleotides (<15 bases). Serological analyses of the expression of distinct idiotypic determinants possessed by purine and pyrimidine specific antibodies indicated the potential for an extensive clonal repertoire.","Thirty-eight monoclonal anti-fluorescein antibodies were derived from immunized BW mice to evaluate various aspects of induced antibody expression, including isotype and affinity distribution. Monoclonal IgM antibody 18-2-3, produced from an initial BW cell fusion, exhibited low-temperature insolubility and an unusually high hapten-binding affinity (K(,A)) of 2.9 x 10('10) M('-1). Insolubility at low temperature was reversible in the presence of fluorescyl ligand, indicative of active site involvement in the mechanism of 18-2-3 cryoprecipitation. All other IgM and IgG proteins were restricted in hapten-binding affinities (K(,A) < 7 x 10('7) M('-1)) and possessed normal solubility properties, suggesting that 18-2-3 was derived from a relatively rare B cell progenitor. Relative subclass frequencies for 30 monoclonal IgG proteins were consistent with those reported for polyclonal thymic-dependent responses in normal strains, thus providing no evidence that T cells involved in regulation of IgG subclass expression are compromised in autoimmune BW mice.","Made available in DSpace on 2014-12-16T06:12:54Z (GMT). No. of bitstreams: 1 8511577.pdf: 8746489 bytes, checksum: bb354416bd08ba27b4f6e54a6d28a793 (MD5) Previous issue date: 1985","Embargo set by: Seth Robbins for item 71335 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","290 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1985."]},{"key":"dc:title","label":"Title","values":["Monoclonal Analysis of Spontaneous and Induced Antibody Responses in Autoimmune New Zealand Mice"]}]}],"canonical_facts":{"dc:creator":["Ballard, Dean Williams"],"dc:date":["2014-12-16T06:12:54Z","10000-01-01","1985"],"dc:description":["New Zealand BW mice spontaneously develop lymphocytic disorders and express autoantibodies in association with an autoimmune disease similar to human SLE. A comparative monoclonal analysis of spontaneous and experimentally-induced antibody responses in autoimmune BW mice was facilitated by hybridoma technology. Various immunochemical and physicochemical parameters of generated monoclonal antibodies were examined to provide a qualitative functional and structural description of such etiologically distinct antibody repertoires in the context of inherent cellular and humoral abnormalities characteristic of this strain.","Ten monoclonal anti-DNA autoantibodies, derived from nonmanipulated BW mice and specific for various forms of DNA, were found to contain either IgG2a or IgG2b heavy chains related by a cross-reactive allotypic determinant. Binding studies, which employed defined DNA restriction fragments and synthetic nucleic acids, indicated that: (1) both helical conformation and nucleotide composition were involved in antibody recognition of duplex and single-stranded DNA, and (2) anti-DNA antibodies formed stable complexes with short synthetic oligonucleotides (<15 bases). Serological analyses of the expression of distinct idiotypic determinants possessed by purine and pyrimidine specific antibodies indicated the potential for an extensive clonal repertoire.","Thirty-eight monoclonal anti-fluorescein antibodies were derived from immunized BW mice to evaluate various aspects of induced antibody expression, including isotype and affinity distribution. Monoclonal IgM antibody 18-2-3, produced from an initial BW cell fusion, exhibited low-temperature insolubility and an unusually high hapten-binding affinity (K(,A)) of 2.9 x 10('10) M('-1). Insolubility at low temperature was reversible in the presence of fluorescyl ligand, indicative of active site involvement in the mechanism of 18-2-3 cryoprecipitation. All other IgM and IgG proteins were restricted in hapten-binding affinities (K(,A) < 7 x 10('7) M('-1)) and possessed normal solubility properties, suggesting that 18-2-3 was derived from a relatively rare B cell progenitor. Relative subclass frequencies for 30 monoclonal IgG proteins were consistent with those reported for polyclonal thymic-dependent responses in normal strains, thus providing no evidence that T cells involved in regulation of IgG subclass expression are compromised in autoimmune BW mice.","Made available in DSpace on 2014-12-16T06:12:54Z (GMT). No. of bitstreams: 1 8511577.pdf: 8746489 bytes, checksum: bb354416bd08ba27b4f6e54a6d28a793 (MD5) Previous issue date: 1985","Embargo set by: Seth Robbins for item 71335 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","290 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1985."],"dc:identifier":["http://hdl.handle.net/2142/71169","(UMI)AAI8511577"],"dc:subject":["Health Sciences, Immunology"],"dc:title":["Monoclonal Analysis of Spontaneous and Induced Antibody Responses in Autoimmune New Zealand Mice"],"dc:type":["text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:04Z"}