Novel Compounds From Methanogens: Characterization of Component B of the Methylreductase System and Mobile Factor
type: CreativeWork
description: An enzymic assay for the detection and quantitation of component B based on cell-free extract of Methanobacterium thermoautotrophicum stripped of small molecular weight cofactors by anaerobic column chromatography was developed. Component B was purified to homogeneity from boiled cell extract of M. thermoautotrophicum by anaerobic chromatography on Sephadex DEAE-A25, Sephadex G10, and Sephadex G25-F columns. Component B was determined to have a molecular weight of 1,044 by fast atom bombardment mass spectrometry, and a molecular formula of C(,32)H(,47)N(,3)O(,26)P(,2)Na(,4) was proposed for the sodium salt of the compound. A primary amine was present in component B, but no amino acid was detected. Component B was determined to have a monoester phosphate and a diester phosphate, and one mol of aldopentose per mol of component B. The UV absorbance spectrum revealed the presence of two absorbance peaks in component B: one at 267 nm and the other at 233 nm. It was concluded that component B contained a nucleotide moiety.
abstract: An enzymic assay for the detection and quantitation of component B based on cell-free extract of Methanobacterium thermoautotrophicum stripped of small molecular weight cofactors by anaerobic column chromatography was developed. Component B was purified to homogeneity from boiled cell extract of M. thermoautotrophicum by anaerobic chromatography on Sephadex DEAE-A25, Sephadex G10, and Sephadex G25-F columns. Component B was determined to have a molecular weight of 1,044 by fast atom bombardment mass spectrometry, and a molecular formula of C(,32)H(,47)N(,3)O(,26)P(,2)Na(,4) was proposed for the sodium salt of the compound. A primary amine was present in component B, but no amino acid was detected. Component B was determined to have a monoester phosphate and a diester phosphate, and one mol of aldopentose per mol of component B. The UV absorbance spectrum revealed the presence of two absorbance peaks in component B: one at 267 nm and the other at 233 nm. It was concluded that component B contained a nucleotide moiety.
author: Tanner, Ralph S.
keywords: Biology, Microbiology
date published: 2014-12-16T06:12:48Z
date modified: 2026-07-22T22:26:04Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/71156