{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/71156"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/71156","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Novel Compounds From Methanogens: Characterization of Component B of the Methylreductase System and Mobile Factor","abstract":"An enzymic assay for the detection and quantitation of component B based on cell-free extract of Methanobacterium thermoautotrophicum stripped of small molecular weight cofactors by anaerobic column chromatography was developed. Component B was purified to homogeneity from boiled cell extract of M. thermoautotrophicum by anaerobic chromatography on Sephadex DEAE-A25, Sephadex G10, and Sephadex G25-F columns. Component B was determined to have a molecular weight of 1,044 by fast atom bombardment mass spectrometry, and a molecular formula of C(,32)H(,47)N(,3)O(,26)P(,2)Na(,4) was proposed for the sodium salt of the compound. A primary amine was present in component B, but no amino acid was detected. Component B was determined to have a monoester phosphate and a diester phosphate, and one mol of aldopentose per mol of component B. The UV absorbance spectrum revealed the presence of two absorbance peaks in component B: one at 267 nm and the other at 233 nm. It was concluded that component B contained a nucleotide moiety.","abstract_html":"An enzymic assay for the detection and quantitation of component B based on cell-free extract of Methanobacterium thermoautotrophicum stripped of small molecular weight cofactors by anaerobic column chromatography was developed. Component B was purified to homogeneity from boiled cell extract of M. thermoautotrophicum by anaerobic chromatography on Sephadex DEAE-A25, Sephadex G10, and Sephadex G25-F columns. Component B was determined to have a molecular weight of 1,044 by fast atom bombardment mass spectrometry, and a molecular formula of C(,32)H(,47)N(,3)O(,26)P(,2)Na(,4) was proposed for the sodium salt of the compound. A primary amine was present in component B, but no amino acid was detected. Component B was determined to have a monoester phosphate and a diester phosphate, and one mol of aldopentose per mol of component B. The UV absorbance spectrum revealed the presence of two absorbance peaks in component B: one at 267 nm and the other at 233 nm. It was concluded that component B contained a nucleotide moiety.","abstract_has_math":false,"creators":["Tanner, Ralph S."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-16T06:12:48Z","date_published":"2014-12-16T06:12:48Z","updated_at":"2026-07-22T22:26:04Z","subjects":["Biology, Microbiology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8303004"],"render_values":[{"text":"(UMI)AAI8303004","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/71156","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Tanner, Ralph S."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-16T06:12:48Z","10000-01-01","1982"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Microbiology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/71156","(UMI)AAI8303004"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["An enzymic assay for the detection and quantitation of component B based on cell-free extract of Methanobacterium thermoautotrophicum stripped of small molecular weight cofactors by anaerobic column chromatography was developed. Component B was purified to homogeneity from boiled cell extract of M. thermoautotrophicum by anaerobic chromatography on Sephadex DEAE-A25, Sephadex G10, and Sephadex G25-F columns. Component B was determined to have a molecular weight of 1,044 by fast atom bombardment mass spectrometry, and a molecular formula of C(,32)H(,47)N(,3)O(,26)P(,2)Na(,4) was proposed for the sodium salt of the compound. A primary amine was present in component B, but no amino acid was detected. Component B was determined to have a monoester phosphate and a diester phosphate, and one mol of aldopentose per mol of component B. The UV absorbance spectrum revealed the presence of two absorbance peaks in component B: one at 267 nm and the other at 233 nm. It was concluded that component B contained a nucleotide moiety.","The function of component B was not determined. Cyanocobalamin was found to greatly stimulate methylreductase activity in the component B assay system, but the cause of the stimulation was not determined.","A defined medium was developed for Methanomicrobium mobile; this made possible the study of mobile factor, which is required for the growth of M. mobile. M. mobile was found to require acetate, isobutyrate, 2-methylbutyrate, isovalerate, tryptophan, para-aminobenzoic acid, pyridoxine, thiamine, biotin, and vitamin B(,12) for its growth. Sources of the branched-chain amino acids would not substitute for the branched-chain volatile fatty acids. Indole would substitute for tryptophan, but skatole, anthranilic acid, shikimic acid, or 3-indolelactic acid could not substitute. Folic acid could not substitute for para-aminobenzoic acid. Mobile factor was partially purified by anaerobic chromatography on a Sephadex DEAE-A25 column and a Dowex 50W-X4 column.","Made available in DSpace on 2014-12-16T06:12:48Z (GMT). No. of bitstreams: 1 8303004.pdf: 2966521 bytes, checksum: 6cdf1a7182c88014da739ad0eae9cde5 (MD5) Previous issue date: 1982","Embargo set by: Seth Robbins for item 71322 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","106 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1982."]},{"key":"dc:title","label":"Title","values":["Novel Compounds From Methanogens: Characterization of Component B of the Methylreductase System and Mobile Factor"]}]}],"canonical_facts":{"dc:creator":["Tanner, Ralph S."],"dc:date":["2014-12-16T06:12:48Z","10000-01-01","1982"],"dc:description":["An enzymic assay for the detection and quantitation of component B based on cell-free extract of Methanobacterium thermoautotrophicum stripped of small molecular weight cofactors by anaerobic column chromatography was developed. Component B was purified to homogeneity from boiled cell extract of M. thermoautotrophicum by anaerobic chromatography on Sephadex DEAE-A25, Sephadex G10, and Sephadex G25-F columns. Component B was determined to have a molecular weight of 1,044 by fast atom bombardment mass spectrometry, and a molecular formula of C(,32)H(,47)N(,3)O(,26)P(,2)Na(,4) was proposed for the sodium salt of the compound. A primary amine was present in component B, but no amino acid was detected. Component B was determined to have a monoester phosphate and a diester phosphate, and one mol of aldopentose per mol of component B. The UV absorbance spectrum revealed the presence of two absorbance peaks in component B: one at 267 nm and the other at 233 nm. It was concluded that component B contained a nucleotide moiety.","The function of component B was not determined. Cyanocobalamin was found to greatly stimulate methylreductase activity in the component B assay system, but the cause of the stimulation was not determined.","A defined medium was developed for Methanomicrobium mobile; this made possible the study of mobile factor, which is required for the growth of M. mobile. M. mobile was found to require acetate, isobutyrate, 2-methylbutyrate, isovalerate, tryptophan, para-aminobenzoic acid, pyridoxine, thiamine, biotin, and vitamin B(,12) for its growth. Sources of the branched-chain amino acids would not substitute for the branched-chain volatile fatty acids. Indole would substitute for tryptophan, but skatole, anthranilic acid, shikimic acid, or 3-indolelactic acid could not substitute. Folic acid could not substitute for para-aminobenzoic acid. Mobile factor was partially purified by anaerobic chromatography on a Sephadex DEAE-A25 column and a Dowex 50W-X4 column.","Made available in DSpace on 2014-12-16T06:12:48Z (GMT). No. of bitstreams: 1 8303004.pdf: 2966521 bytes, checksum: 6cdf1a7182c88014da739ad0eae9cde5 (MD5) Previous issue date: 1982","Embargo set by: Seth Robbins for item 71322 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","106 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1982."],"dc:identifier":["http://hdl.handle.net/2142/71156","(UMI)AAI8303004"],"dc:subject":["Biology, Microbiology"],"dc:title":["Novel Compounds From Methanogens: Characterization of Component B of the Methylreductase System and Mobile Factor"],"dc:type":["text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:04Z"}